PMID- 32439322
OWN - NLM
STAT- MEDLINE
DCOM- 20210212
LR  - 20210212
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 101
DP  - 2020 Jul
TI  - Dysplastic lipoma: potential diagnostic pitfall of using MDM2 RNA in situ 
      hybridization to distinguish between lipoma and atypical lipomatous tumor.
PG  - 53-57
LID - S0046-8177(20)30097-6 [pii]
LID - 10.1016/j.humpath.2020.05.004 [doi]
AB  - The distinction between lipoma and atypical lipomatous tumor can be challenging 
      in some cases. While detection of MDM2 gene amplification via fluorescence in 
      situ hybridization (FISH) has been well established as a diagnostic tool to 
      distinguish atypical lipomatous tumor and well-differentiated liposarcoma from 
      benign mimics, MDM2 RNA in situ hybridization (RNA-ISH) has recently been 
      proposed as an alternative diagnostic assay. During clinical workup for 
      lipomatous tumors using MDM2 RNA-ISH, we noticed several dysplastic lipomas that 
      were positive for MDM2 RNA-ISH but negative for MDM2 amplification by FISH. In 
      this study, we examined a series of 11 dysplastic lipomas, all confirmed to be 
      negative for MDM2 amplification by FISH. Positive MDM2 RNA-ISH was noted in 10 
      (91%) dysplastic lipomas. Single-nucleotide polymorphism array on one dysplastic 
      lipoma identified the presence of homozygous deletion of 13q, including the RB1 
      gene locus with no evidence of MDM2 copy number gain. Our findings on the 
      discordance between MDM2 FISH and MDM2 RNA-ISH highlight the potential utility 
      and pitfalls of using MDM2 RNA-ISH in the distinction of atypical lipomatous 
      tumor and related liposarcomas from dysplastic lipoma.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Hung, Yin P
AU  - Hung YP
AD  - Department of Pathology, Massachusetts General Hospital and Harvard Medical 
      School, 55 Fruit Street, Boston, MA, 02114, United States. Electronic address: 
      yphung@mgh.harvard.edu.
FAU - Michal, Michael
AU  - Michal M
AD  - Department of Pathology, Faculty of Medicine in Plzen, Charles University, Plzen, 
      32300, Czech Republic; Biomedical Center, Faculty of Medicine in Plzen, Charles 
      University, Plzen, 32300, Czech Republic. Electronic address: 
      michael.michal@biopticka.cz.
FAU - Dubuc, Adrian M
AU  - Dubuc AM
AD  - Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, 
      Boston, MA, 02115, United States.
FAU - Rosenberg, Andrew E
AU  - Rosenberg AE
AD  - Department of Pathology and Laboratory Medicine, Miller School of Medicine, 
      University of Miami, Miami, FL, 33136, United States. Electronic address: 
      ARosenberg@med.miami.edu.
FAU - Nielsen, G Petur
AU  - Nielsen GP
AD  - Department of Pathology, Massachusetts General Hospital and Harvard Medical 
      School, 55 Fruit Street, Boston, MA, 02114, United States. Electronic address: 
      gnielsen@mgh.harvard.edu.
LA  - eng
PT  - Journal Article
DEP - 20200518
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Biomarkers, Tumor)
RN  - 63231-63-0 (RNA)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Diagnosis, Differential
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Lipoma/*diagnosis/genetics
MH  - Liposarcoma/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-mdm2/*analysis/genetics
MH  - RNA/analysis
MH  - Young Adult
OTO - NOTNLM
OT  - Anisometric lipoma
OT  - Atypical lipomatous tumor
OT  - Dysplastic lipoma
OT  - In situ hybridization
OT  - MDM2
EDAT- 2020/05/23 06:00
MHDA- 2021/02/13 06:00
CRDT- 2020/05/23 06:00
PHST- 2020/03/17 00:00 [received]
PHST- 2020/05/02 00:00 [revised]
PHST- 2020/05/06 00:00 [accepted]
PHST- 2020/05/23 06:00 [pubmed]
PHST- 2021/02/13 06:00 [medline]
PHST- 2020/05/23 06:00 [entrez]
AID - S0046-8177(20)30097-6 [pii]
AID - 10.1016/j.humpath.2020.05.004 [doi]
PST - ppublish
SO  - Hum Pathol. 2020 Jul;101:53-57. doi: 10.1016/j.humpath.2020.05.004. Epub 2020 May 
      18.