PMID- 32440237
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220414
IS  - 1179-1578 (Print)
IS  - 1179-1578 (Electronic)
IS  - 1179-1578 (Linking)
VI  - 13
DP  - 2020
TI  - Chronic Restraint Stress Induces Gastric Mucosal Inflammation with Enhanced 
      Oxidative Stress in a Murine Model.
PG  - 383-393
LID - 10.2147/PRBM.S250945 [doi]
AB  - BACKGROUND: Although the underlying mechanisms of chronic stress are still 
      unknown, this condition has been related to the pathophysiology of gastric 
      mucosal inflammation, whose development is accelerated by oxidative stress. The 
      present study investigates how chronic stress influences gastric mucosal 
      oxidative stress and inflammation. METHODS: Eight-week-old C57BL/6J male mice 
      were subjected to two-week intermittent restraint stress. The expressions of 
      CD11b (a specific for monocyte/macrophage), monocyte/macrophage cell surface 
      markers (CD68 and F4/80), NADPH oxidase-4 (Nox-4) and 8-hydroxy-2'-deoxyguanosine 
      (8-OHdG, a sensitive biomarker of oxidative stress) were determined using 
      immunohistochemistry, RT-PCR, and enzyme-linked immunosorbent assay, 
      respectively. The expressions of antioxidant enzymes, such as superoxide 
      dismutase, catalase, and glutathione peroxidase, were examined by RT-PCR and 
      Western blotting. The expressions of proinflammatory cytokines, including 
      monocyte chemoattractant protein-1 (MCP-1), interleukin-1beta (IL-1beta), and tumor 
      necrosis factor-alpha (TNF-alpha), were determined using immunohistochemistry and RT-PCR, 
      respectively. RESULTS: Chronic stress increased the lymphocytic infiltration and 
      inflammation within the gastric mucosa of mice. Stress remarkably increased the 
      expression levels of CD11b and mRNA expression levels of CD68 and F4/80 in the 
      mucosa of the stomach of stressed mice. Stress remarkably increased both mRNA and 
      plasma concentrations of Nox-4 and 8-OHdG; and markedly reduced gastric mRNA and 
      protein expression levels of antioxidant enzymes such as superoxide dismutase, 
      catalase, and glutathione peroxidase. The expressions of proinflammatory 
      cytokines (MCP-1, IL-1beta, and TNF-alpha) were predominantly observed in the gastric 
      mucosal layers of the stressed mice. Furthermore, stress remarkably elevated the 
      gastric mucosal mRNA expression levels of MCP-1, IL-1beta, and TNF-alpha. CONCLUSION: 
      Two weeks of restraint stress induced gastric inflammation in the murine model 
      with enhanced oxidative stress and reduced anti-oxidative system.
CI  - (c) 2020 Yisireyili et al.
FAU - Yisireyili, Maimaiti
AU  - Yisireyili M
AD  - Research Institute of General and Minimally Invasive Surgery, People's Hospital 
      of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, People's Republic 
      of China.
AD  - Department of Minimally Invasive Surgery, Hernia, and Abdominal Wall Surgery, 
      People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, 
      People's Republic of China.
FAU - Alimujiang, Aziguli
AU  - Alimujiang A
AD  - Research Institute of General and Minimally Invasive Surgery, People's Hospital 
      of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, People's Republic 
      of China.
FAU - Aili, Aikebaier
AU  - Aili A
AD  - Research Institute of General and Minimally Invasive Surgery, People's Hospital 
      of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, People's Republic 
      of China.
AD  - Department of Minimally Invasive Surgery, Hernia, and Abdominal Wall Surgery, 
      People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, 
      People's Republic of China.
FAU - Li, Yiliang
AU  - Li Y
AD  - Department of Minimally Invasive Surgery, Hernia, and Abdominal Wall Surgery, 
      People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, 
      People's Republic of China.
FAU - Yisireyili, Salamaiti
AU  - Yisireyili S
AD  - Research Institute of General and Minimally Invasive Surgery, People's Hospital 
      of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, People's Republic 
      of China.
FAU - Abudureyimu, Kelimu
AU  - Abudureyimu K
AUID- ORCID: 0000-0003-3418-7304
AD  - Research Institute of General and Minimally Invasive Surgery, People's Hospital 
      of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, People's Republic 
      of China.
AD  - Department of Minimally Invasive Surgery, Hernia, and Abdominal Wall Surgery, 
      People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830001, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20200504
PL  - New Zealand
TA  - Psychol Res Behav Manag
JT  - Psychology research and behavior management
JID - 101514563
PMC - PMC7210023
OTO - NOTNLM
OT  - anti-oxidative proteins
OT  - gastric mucosal inflammation
OT  - oxidative stress
OT  - stress
COIS- The authors report no conflicts of interest in this work.
EDAT- 2020/05/23 06:00
MHDA- 2020/05/23 06:01
PMCR- 2020/05/04
CRDT- 2020/05/23 06:00
PHST- 2020/02/23 00:00 [received]
PHST- 2020/04/16 00:00 [accepted]
PHST- 2020/05/23 06:00 [entrez]
PHST- 2020/05/23 06:00 [pubmed]
PHST- 2020/05/23 06:01 [medline]
PHST- 2020/05/04 00:00 [pmc-release]
AID - 250945 [pii]
AID - 10.2147/PRBM.S250945 [doi]
PST - epublish
SO  - Psychol Res Behav Manag. 2020 May 4;13:383-393. doi: 10.2147/PRBM.S250945. 
      eCollection 2020.