PMID- 32441107
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20220716
IS  - 1947-6043 (Electronic)
IS  - 1947-6035 (Print)
IS  - 1947-6035 (Linking)
VI  - 13
IP  - 2_suppl
DP  - 2021 Dec
TI  - Chondroitinase ABC Enhances Integration of Self-Assembled Articular Cartilage, 
      but Its Dosage Needs to Be Moderated Based on Neocartilage Maturity.
PG  - 672S-683S
LID - 10.1177/1947603520918653 [doi]
AB  - OBJECTIVE: To enhance the in vitro integration of self-assembled articular 
      cartilage to native articular cartilage using chondroitinase ABC. DESIGN: To 
      examine the hypothesis that chondroitinase ABC (C-ABC) integration treatment 
      (C-ABC(int)) would enhance integration of neocartilage of different maturity 
      levels, this study was conducted in 2 phases. In phase I, the impact on 
      integration of 2 treatments, TCL (TGF-beta1, C-ABC, and lysyl oxidase like 2) and 
      C-ABC(int), was examined via a 2-factor, full factorial design. In phase II, 
      construct maturity (2 levels) and C-ABC(int) concentration (3 levels) were the 
      factors in a full factorial design to determine whether the effective C-ABC(int) 
      dose was dependent on neocartilage maturity level. Neocartilages formed or 
      treated per the factors above were placed into native cartilage rings, cultured 
      for 2 weeks, and, then, integration was studied histologically and mechanically. 
      Prior to integration, in phase II, a set of treated constructs were also assayed 
      to provide a baseline of properties. RESULTS: In phase I, C-ABC(int) and TCL 
      treatments synergistically enhanced interface Young's modulus by 6.2-fold (P = 
      0.004) and increased interface tensile strength by 3.8-fold (P = 0.02) compared 
      with control. In phase II, the interaction of the factors C-ABC(int) and 
      construct maturity was significant (P = 0.0004), indicating that the effective 
      C-ABC(int) dose to improve interface Young's modulus is dependent on construct 
      maturity. Construct mechanical properties were preserved regardless of C-ABC(int) 
      dose. CONCLUSIONS: Applying C-ABC(int) to neocartilage is an effective 
      integration strategy with translational potential, provided its dose is 
      calibrated appropriately based on implant maturity, that also preserves implant 
      biomechanical properties.
FAU - Link, Jarrett M
AU  - Link JM
AUID- ORCID: 0000-0001-9108-1456
AD  - Department of Biomedical Engineering, University of California, Irvine, CA, USA.
FAU - Hu, Jerry C
AU  - Hu JC
AD  - Department of Biomedical Engineering, University of California, Irvine, CA, USA.
FAU - Athanasiou, Kyriacos A
AU  - Athanasiou KA
AD  - Department of Biomedical Engineering, University of California, Irvine, CA, USA.
LA  - eng
GR  - R01 AR067821/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200522
PL  - United States
TA  - Cartilage
JT  - Cartilage
JID - 101518378
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - *Cartilage, Articular
MH  - Chondrocytes
MH  - Chondroitin ABC Lyase
MH  - Tensile Strength
MH  - Tissue Engineering
PMC - PMC8804832
OTO - NOTNLM
OT  - biomechanics
OT  - cartilage tissue engineering
OT  - chondroitinase ABC
OT  - integration
OT  - self-assembled articular cartilage
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2020/05/23 06:00
MHDA- 2022/04/07 06:00
PMCR- 2022/06/01
CRDT- 2020/05/23 06:00
PHST- 2020/05/23 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2020/05/23 06:00 [entrez]
PHST- 2022/06/01 00:00 [pmc-release]
AID - 10.1177_1947603520918653 [pii]
AID - 10.1177/1947603520918653 [doi]
PST - ppublish
SO  - Cartilage. 2021 Dec;13(2_suppl):672S-683S. doi: 10.1177/1947603520918653. Epub 
      2020 May 22.