PMID- 32442921
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240227
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1148
DP  - 2020 Mar 30
TI  - Development of an immobilized liposome chromatography method for screening and 
      characterizing alpha-glucosidase-binding compounds.
PG  - 122097
LID - S1570-0232(19)31638-1 [pii]
LID - 10.1016/j.jchromb.2020.122097 [doi]
AB  - Immobilized liposome chromatography (ILC) is a powerful tool in screening and 
      analyzing membrane-permeable components in natural medicinal herbs. In this 
      study, the stationary phase of a new receptor liposome biomembrane chromatography 
      (RLBC) was employed to screen the active ingredients in seven natural medicinal 
      herbs. As a model system, alpha-glucosidase was immobilized in porous silica gel by 
      incorporating alpha-glucosidase into liposome vesicles. Combined with HPLC, this 
      stationary phase can be used to evaluate the interaction of liposome-protein 
      compounds with compounds and drugs. The surface characteristics of the RLBC phase 
      was characterized by Scanning electron microscope (SEM), Fourier transform 
      infrared spectroscopy (FTIR) and Energy dispersive spectrometer (EDS). RLBC was 
      successfully used to screen and analyze permeable compounds in natural medicinal 
      herbs, and screen the extracts from Schisandra chinensis. This method was used to 
      establish that Schisandrin in Schisandra chinensis is an active component. 
      Furthermore, the hypoglycemic effect of Schisandrin was verified in vivo in rats. 
      This study further modeled the relationship between the activity of inhibitor and 
      retention behavior with RLBC with a mathematical equation. RLBC stationary phase 
      combined with HPLC can be used for rapid screening of drug candidates.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Hou, Xiaorong
AU  - Hou X
AD  - College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 
      310014, China.
FAU - Lou, Xiaoyi
AU  - Lou X
AD  - College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 
      310014, China.
FAU - Guo, Qian
AU  - Guo Q
AD  - College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 
      310014, China.
FAU - Tang, Lan
AU  - Tang L
AD  - College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 
      310014, China.
FAU - Shan, Weiguang
AU  - Shan W
AD  - College of Pharmacy, Zhejiang University of Technology, Hangzhou, Zhejiang 
      310014, China. Electronic address: swg@zjut.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200330
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
SB  - IM
OTO - NOTNLM
OT  - Drug screening
OT  - Immobilized enzyme liposome chromatography
OT  - alpha-Glucosidase
OT  - alpha-Glucosidase receptor
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper. Declaration of Competing Interest 
      Authors have declared no conflicts of interest.
EDAT- 2020/05/23 06:00
MHDA- 2020/05/23 06:01
CRDT- 2020/05/23 06:00
PHST- 2019/11/05 00:00 [received]
PHST- 2020/03/12 00:00 [revised]
PHST- 2020/03/29 00:00 [accepted]
PHST- 2020/05/23 06:01 [medline]
PHST- 2020/05/23 06:00 [pubmed]
PHST- 2020/05/23 06:00 [entrez]
AID - S1570-0232(19)31638-1 [pii]
AID - 10.1016/j.jchromb.2020.122097 [doi]
PST - aheadofprint
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Mar 30;1148:122097. doi: 
      10.1016/j.jchromb.2020.122097.