PMID- 32444923
OWN - NLM
STAT- MEDLINE
DCOM- 20210603
LR  - 20210603
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 45
IP  - 8
DP  - 2020 Aug
TI  - beta-Caryophyllene Liposomes Attenuate Neurovascular Unit Damage After Subarachnoid 
      Hemorrhage in Rats.
PG  - 1758-1768
LID - 10.1007/s11064-020-03037-8 [doi]
AB  - This study was conducted to prepare beta-caryophyllene loaded liposomes (BCP-LP) and 
      investigated their effects on neurovascular unit (NVU) damage after subarachnoid 
      hemorrhage (SAH) in rats. A blood injection into the pre-chiasmatic cistern was 
      used to achieve SAH. BCP-LP were prepared, characterized and administrated to 
      rats with SAH. The prepared BCP-LP were spherical with a size distribution of 
      approximately 189.3 nm and Zeta potential of - 13.9 mV. Neurological scoring, the 
      balance beam test, cerebral blood flow monitoring, brain edema and biochemical 
      analyses were applied to evaluate the effects of BCP-LP on rat NVU damage after 
      SAH. The results demonstrated that BCP-LP treatment improved neurological 
      function disorder, balance ability and cerebral blood perfusion in rats. Brain 
      edema detection and blood-brain barrier permeability detection revealed that 
      BCP-LP could reduce brain edema and promote repairment of blood-brain barrier 
      after SAH. Using the western blot experiments, we demonstrated that BCP-LP 
      attenuated the loss of tight junction proteins Occludin and Zonula occludens-1, 
      inhibit the high expression of VEGFR-2 and GFAP, and promote the repair of 
      laminin. These results demonstrate the protective effect BCP-LP exert in the NVU 
      after SAH in rats, and supports the use of BCP-LP for future study and therapy of 
      SAH.
FAU - Wang, Fang
AU  - Wang F
AD  - Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, School of 
      Pharmacy, Chongqing Medical University, District of Yuzhong, Chongqing, 400016, 
      People's Republic of China.
FAU - Teng, Zhipeng
AU  - Teng Z
AD  - Department of Neurosurgery, Chongqing Traditional Chinese Medicine Hospital, 
      Chongqing, 400021, People's Republic of China.
FAU - Liu, Daohang
AU  - Liu D
AD  - Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, School of 
      Pharmacy, Chongqing Medical University, District of Yuzhong, Chongqing, 400016, 
      People's Republic of China.
FAU - Wang, Yuchun
AU  - Wang Y
AD  - Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, School of 
      Pharmacy, Chongqing Medical University, District of Yuzhong, Chongqing, 400016, 
      People's Republic of China.
FAU - Lou, Jie
AU  - Lou J
AD  - School of Pharmacy and Bioengineering, Chongqing University of Technology, 
      Chongqing, 400054, People's Republic of China. loujie1210@126.com.
FAU - Dong, Zhi
AU  - Dong Z
AD  - Chongqing Key Laboratory of Biochemistry & Molecular Pharmacology, School of 
      Pharmacy, Chongqing Medical University, District of Yuzhong, Chongqing, 400016, 
      People's Republic of China. dongzhidyx@163.com.
LA  - eng
GR  - cstc2018jxjl130049/Special project of incentives and guidance for scientific 
      research institutions in Chongqing/
GR  - ZY201702014/Traditional Chinese Medicine Science and Technology Project of 
      Chongqing municipal health and Health Committee/
GR  - YYZX20180045/Special Project of Chengdu University of Traditional Chinese 
      Medicine/
PT  - Journal Article
DEP - 20200522
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Drug Carriers)
RN  - 0 (GFAP protein, rat)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Liposomes)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Polycyclic Sesquiterpenes)
RN  - BHW853AU9H (caryophyllene)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/drug effects
MH  - Brain Edema/epidemiology/prevention & control
MH  - Drug Carriers/*chemistry
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Liposomes/*chemistry
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Neuroprotective Agents/*therapeutic use
MH  - Polycyclic Sesquiterpenes/*therapeutic use
MH  - Rats, Sprague-Dawley
MH  - Subarachnoid Hemorrhage/complications/*prevention & control
OTO - NOTNLM
OT  - Blood brain barrier
OT  - Liposome
OT  - Neurovascular unit
OT  - Subarachnoid hemorrhage (SAH)
OT  - beta-Caryophyllene (BCP)
EDAT- 2020/05/24 06:00
MHDA- 2021/06/04 06:00
CRDT- 2020/05/24 06:00
PHST- 2019/04/20 00:00 [received]
PHST- 2020/04/15 00:00 [accepted]
PHST- 2020/03/30 00:00 [revised]
PHST- 2020/05/24 06:00 [pubmed]
PHST- 2021/06/04 06:00 [medline]
PHST- 2020/05/24 06:00 [entrez]
AID - 10.1007/s11064-020-03037-8 [pii]
AID - 10.1007/s11064-020-03037-8 [doi]
PST - ppublish
SO  - Neurochem Res. 2020 Aug;45(8):1758-1768. doi: 10.1007/s11064-020-03037-8. Epub 
      2020 May 22.