PMID- 32445810
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1873-507X (Electronic)
IS  - 0031-9384 (Linking)
VI  - 222
DP  - 2020 Aug 1
TI  - Sexual experience with a known male modulates c-Fos expression in response to 
      mating and male pheromone exposure in female mice.
PG  - 112906
LID - S0031-9384(20)30221-3 [pii]
LID - 10.1016/j.physbeh.2020.112906 [doi]
AB  - Sexually naive female mice are not sexually receptive in their first mating 
      opportunity. Four to five sexual encounters are needed to display high sexual 
      receptivity as assessed by the lordosis reflex. The neuronal changes induced by 
      sexual experience are not well understood. In this study, we evaluated if 
      repeated sexual stimulation with the same male was associated with an increase in 
      the neuronal activity evaluated by c-Fos expression in brain structures 
      associated with the control of sexual behavior such as the accessory olfactory 
      bulb (AOB), ventromedial hypothalamus (VMH), and the medial preoptic area (MPOA). 
      Ovariectomized female mice were randomly distributed into three groups: sexually 
      naive (SN), with no prior sexual stimulation; sexually inexperienced (SI), with 
      one prior mating session; and sexually experienced (SE), with six prior mating 
      sessions. Females were primed with estradiol benzoate and progesterone once a 
      week for 7 weeks. Neuronal activation in response to mating or soiled bedding was 
      evaluated in the 7(th) week. Each group was subdivided into three subgroups: 
      clean (exposure to clean bedding), male bedding (exposure to sawdust soiled with 
      secretions from a male), or mating. Each female mated with her assigned male; in 
      the exposure subgroup, soiled bedding was obtained from the male with whom she 
      mated. Neuronal activity data showed that SE females had a higher c-Fos response 
      in the VMH when they mated in comparison to females exposed to clean bedding. SI 
      females that mated had a decrease c-Fos expression in the glomerular cell layer 
      of the AOB, compared to females exposed to male bedding. The mitral cell layer 
      showed a higher c-Fos response in SI females that mated in comparison to those 
      exposed to male bedding. Comparisons between groups presented with the same 
      stimulus indicate that SI females exposed to male bedding showed a decrease in 
      c-Fos response in the mitral cell layer in comparison to SE and SN females. 
      Correlation analysis demonstrated that the lordosis quotient from the last mating 
      test correlated positively with the number of c-Fos-positive cells in the mitral 
      cell layer in SE and SI groups. A similar correlation was found in the MPOA in SI 
      females. Prior mating in female mice is required to increase sexual receptivity. 
      Changes in the neuronal activity in the AOB and VMH may be involved in the 
      neuronal plasticity induced by repeated sexual stimulation.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Marco-Manclus, P
AU  - Marco-Manclus P
AD  - Instituto de Neurobiologia, UNAM. Juriquilla Queretaro, Mexico.
FAU - Paredes, R G
AU  - Paredes RG
AD  - Instituto de Neurobiologia, UNAM. Juriquilla Queretaro, Mexico; Escuela Nacional 
      de Estudios Superiores, Unidad Juriquilla, UNAM. Juriquilla Queretaro, Mexico.
FAU - Portillo, W
AU  - Portillo W
AD  - Instituto de Neurobiologia, UNAM. Juriquilla Queretaro, Mexico. Electronic 
      address: portillo@unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200520
PL  - United States
TA  - Physiol Behav
JT  - Physiology & behavior
JID - 0151504
RN  - 0 (Pheromones)
RN  - 0 (Proto-Oncogene Proteins c-fos)
SB  - IM
MH  - Animals
MH  - Female
MH  - Hypothalamus/metabolism
MH  - Male
MH  - Mice
MH  - Olfactory Bulb/metabolism
MH  - *Pheromones
MH  - Preoptic Area/metabolism
MH  - *Proto-Oncogene Proteins c-fos/metabolism
MH  - Sexual Behavior, Animal
OTO - NOTNLM
OT  - Female receptivity
OT  - Sexual experience
OT  - c-Fos expression
EDAT- 2020/05/24 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/05/24 06:00
PHST- 2019/09/02 00:00 [received]
PHST- 2020/01/25 00:00 [revised]
PHST- 2020/04/03 00:00 [accepted]
PHST- 2020/05/24 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/05/24 06:00 [entrez]
AID - S0031-9384(20)30221-3 [pii]
AID - 10.1016/j.physbeh.2020.112906 [doi]
PST - ppublish
SO  - Physiol Behav. 2020 Aug 1;222:112906. doi: 10.1016/j.physbeh.2020.112906. Epub 
      2020 May 20.