PMID- 32446100
OWN - NLM
STAT- MEDLINE
DCOM- 20200727
LR  - 20200727
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 199
DP  - 2020 Aug
TI  - Graphene oxide size-dependently altered lipid profiles in THP-1 macrophages.
PG  - 110714
LID - S0147-6513(20)30553-4 [pii]
LID - 10.1016/j.ecoenv.2020.110714 [doi]
AB  - Previous studies focused on biocompatibility of graphene oxide (GO) to 
      macrophages, but the impact of GO on lipid profiles in macrophages was less 
      investigated. Herein, we investigated the interactions between THP-1 macrophages 
      and GO of different sizes (GO of size 500-5000 nm, denoted as GO-L; GO of 
      size < 500 nm, denoted as GO-S). We found that after 24 h exposure, the 
      internalization of GO appeared to be minimal, whereas up to 50 mug/mL of GO-L but 
      not GO-S reduced lipid accumulation, accompanying with a significantly reduced 
      release of soluble monocyte chemoattractant protein-1 (MCP-1) but not 
      interleukin-6 (IL-6). Moreover, lipidomic data showed that GO-L decreased the 
      levels of 17 lipid classes, whereas GO-S only decreased the levels of 5 lipid 
      classes. For comparison, 50 mug/mL carbon black (CB) significantly increased lipid 
      accumulation with considerable particle internalization. GO-reduced lipid 
      accumulation was not related with increase of reactive oxygen species (ROS) or 
      induction of autophagy, and modulation of autophagy by chemicals showed no 
      significant effect to alter the effects of GO-L on lipid accumulation. However, 
      exposure to GO reduced the mRNA and protein levels of key components in 
      peroxisome proliferators-activated receptor (PPAR) signaling pathway, a pathway 
      that is related with lipid droplet biogenesis, and the modulation of PPARgamma by 
      chemicals altered the effects of GO-L on lipid accumulation. In conclusion, our 
      results suggested that GO size-dependently altered lipid profiles in THP-1 
      macrophages that might be related with PPAR signaling pathway.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Luo, Yingmei
AU  - Luo Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan, 411105, China.
FAU - Peng, Jinfeng
AU  - Peng J
AD  - School of Mechanical Engineering, Xiangtan University, Xiangtan, 411105, China. 
      Electronic address: pengjinfeng@xtu.edu.cn.
FAU - Huang, Chaobo
AU  - Huang C
AD  - College of Chemical Engineering, Nanjing Forestry University (NFU), Nanjing, 
      210037, China.
FAU - Cao, Yi
AU  - Cao Y
AD  - Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, 
      Xiangtan, 411105, China. Electronic address: caoyi39@xtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200520
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (PPAR gamma)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Autophagy/drug effects
MH  - Cell Survival/drug effects
MH  - Chemokine CCL2/metabolism
MH  - Graphite/*chemistry/*toxicity
MH  - Humans
MH  - Lipid Metabolism/*drug effects
MH  - Macrophages/*drug effects/metabolism/pathology
MH  - PPAR gamma/metabolism
MH  - Particle Size
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction
MH  - THP-1 Cells
OTO - NOTNLM
OT  - Autophagy
OT  - Graphene oxide (GO)
OT  - Lipidomics
OT  - Peroxisome proliferators-activated receptor (PPAR) signaling pathway
OT  - THP-1 macrophage
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/05/24 06:00
MHDA- 2020/07/28 06:00
CRDT- 2020/05/24 06:00
PHST- 2020/01/29 00:00 [received]
PHST- 2020/04/27 00:00 [revised]
PHST- 2020/05/12 00:00 [accepted]
PHST- 2020/05/24 06:00 [pubmed]
PHST- 2020/07/28 06:00 [medline]
PHST- 2020/05/24 06:00 [entrez]
AID - S0147-6513(20)30553-4 [pii]
AID - 10.1016/j.ecoenv.2020.110714 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2020 Aug;199:110714. doi: 10.1016/j.ecoenv.2020.110714. 
      Epub 2020 May 20.