PMID- 32448993
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20200720
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 165
IP  - 8
DP  - 2020 Aug
TI  - RT-qPCR for the diagnosis of the vesiculovirus Cocal virus.
PG  - 1843-1847
LID - 10.1007/s00705-020-04668-6 [doi]
AB  - Cocal virus (COCV) is one of the causative agents of vesicular stomatitis, 
      presenting clinical signs indistinguishable from those caused by foot-and-mouth 
      disease virus (FMDV). Therefore, the differentiation of these two viruses via 
      laboratory diagnosis is essential. The objective of this study was to develop and 
      validate a real-time quantitative PCR (RT-qPCR) protocol for the diagnosis of 
      COCV directly from epithelial samples. The method developed had 97% accuracy at 
      3950 pfu and a repeatability error of 1.29%. RT-qPCR was able to distinguish COCV 
      from other viruses that cause vesicular diseases, an important factor because 
      seroneutralization may produce cross-reactivity between COCV and vesicular 
      stomatitis Alagoas virus (VSAV). No epithelial sample originating from vesicular 
      disease outbreaks between 2014 and 2018 in Brazil was positive for COCV.
FAU - Lazaro Sales, Mariana
AU  - Lazaro Sales M
AUID- ORCID: 0000-0001-9335-9837
AD  - Departamento de Medicina Veterinaria Preventiva da Escola de Veterinaria, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Dall'Agnol, Mariana
AU  - Dall'Agnol M
AD  - Universidade de Brasilia, Brasilia, Brazil.
FAU - de Oliveira, Anapolino Macedo
AU  - de Oliveira AM
AD  - Laboratorio Federal de Defesa Agropecuaria, Pedro Leopoldo, Brazil.
FAU - Camargos, Marcelo Fernandes
AU  - Camargos MF
AD  - Laboratorio Federal de Defesa Agropecuaria, Pedro Leopoldo, Brazil.
FAU - Fonseca, Antonio Augusto Jr
AU  - Fonseca AA Jr
AD  - Laboratorio Federal de Defesa Agropecuaria, Pedro Leopoldo, Brazil.
FAU - Dos Reis, Jenner Karlisson Pimenta
AU  - Dos Reis JKP
AUID- ORCID: 0000-0003-0395-358X
AD  - Departamento de Medicina Veterinaria Preventiva da Escola de Veterinaria, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. jenner@ufmg.br.
LA  - eng
GR  - APQ-03403-18/Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/
PT  - Journal Article
DEP - 20200524
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (RNA, Viral)
RN  - Cocal vesiculovirus
SB  - IM
MH  - Animals
MH  - Brazil
MH  - DNA Viruses/genetics
MH  - Foot-and-Mouth Disease/diagnosis/virology
MH  - Foot-and-Mouth Disease Virus/genetics
MH  - RNA, Viral/genetics
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Vesicular Stomatitis/*diagnosis/*virology
MH  - Vesiculovirus/*genetics
EDAT- 2020/05/26 06:00
MHDA- 2020/07/21 06:00
CRDT- 2020/05/26 06:00
PHST- 2019/12/03 00:00 [received]
PHST- 2020/04/21 00:00 [accepted]
PHST- 2020/05/26 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
PHST- 2020/05/26 06:00 [entrez]
AID - 10.1007/s00705-020-04668-6 [pii]
AID - 10.1007/s00705-020-04668-6 [doi]
PST - ppublish
SO  - Arch Virol. 2020 Aug;165(8):1843-1847. doi: 10.1007/s00705-020-04668-6. Epub 2020 
      May 24.