PMID- 32449963
OWN - NLM
STAT- MEDLINE
DCOM- 20201026
LR  - 20201026
IS  - 1750-3841 (Electronic)
IS  - 0022-1147 (Linking)
VI  - 85
IP  - 6
DP  - 2020 Jun
TI  - Fast quantification of short-chain fatty acids in rat plasma by gas 
      chromatography.
PG  - 1932-1938
LID - 10.1111/1750-3841.15172 [doi]
AB  - Short-chain fatty acids (SCFAs) are the main metabolites of the intestinal flora 
      and play an important role in the interaction between the intestinal flora and 
      host metabolism. Therefore, reliable methods are needed to accurately measure 
      SCFAs concentrations. SCFAs are commonly analyzed by gas chromatography-mass 
      spectrometry (GC-MS), which requires lengthy sample treatments and a long run 
      time. This study aimed to develop a fast GC method with formic acid pretreatment 
      for SCFAs quantification in the plasma of rat. Baseline chromatographic 
      resolution was achieved for three SCFAs (acetic, propionic, and butyric) within 
      an analysis time of 10.5 min. The method exhibited good recovery for a wide range 
      of concentrations with a low limit of detection for each compound. The relative 
      standard deviations (RSDs) of all targeted compounds showed good intra- and 
      interday precision (<10%). We used our method to measure SCFAs levels in plasma 
      samples from rats fed with a high fructose diet (HFD) to test the accuracy of the 
      developed method. It was shown that SCFAs are indeed affected negatively by a HFD 
      (60% fructose). This method was successfully employed to accurately determine 
      SCFAs in the rat plasma with minimum sample preparation. Results showed potential 
      damage of HFD, which produced lower SCFAs. PRACTICAL APPLICATION: Increasingly, 
      microbiota and gut health research are being conducted by many food scientists to 
      elucidate the relationships among the factors of food components, particularly 
      the nondigestible carbohydrates, food processing conditions, and potential health 
      impact. This research provides a useful, rapid, and accurate method that can save 
      time in the analysis of short-chain fatty acids, which are commonly analyzed in 
      gut health research.
CI  - (c) 2020 Institute of Food Technologists(R).
FAU - Wang, Chung-Yi
AU  - Wang CY
AD  - Department of Seafood Science, National Kaohsiung University of Science and 
      Technology, Kaohsiung, Taiwan, 811, ROC.
FAU - Chen, Yu-Wei
AU  - Chen YW
AD  - Department of Medicine, Chang Gung University, Linkou, Taiwan, 333, ROC.
FAU - Tain, You-Lin
AU  - Tain YL
AD  - Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung 
      Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, 
      Taiwan, 833, ROC.
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan, 833, ROC.
FAU - Chang, Sam K C
AU  - Chang SKC
AD  - Experimental Seafood Processing Laboratory, Costal Research and Extension Center, 
      Mississippi State University, Mississippi, MS, 39567, USA.
AD  - Department of Food Science, Nutrition and Health Promotion, Mississippi State 
      University, Mississippi, MS, 39762, USA.
FAU - Huang, Li-Tung
AU  - Huang LT
AD  - Department of Medicine, Chang Gung University, Linkou, Taiwan, 333, ROC.
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan, 833, ROC.
FAU - Hsieh, Chang-Wei
AU  - Hsieh CW
AD  - Department of Food Science and Biotechnology, National Chung Hsing University, 
      145 Xingda Rd., South Dist., Taichung, Taiwan, 402, ROC.
AD  - Department of Medical Research, China Medical University Hospital, Taichung, 
      Taiwan, 404, ROC.
FAU - Hou, Chih-Yao
AU  - Hou CY
AUID- ORCID: 0000-0002-8007-6077
AD  - Department of Seafood Science, National Kaohsiung University of Science and 
      Technology, Kaohsiung, Taiwan, 811, ROC.
LA  - eng
GR  - CMRPG8F0023/Kaohsiung Chang Gung Memorial Hospital, Taiwan/
GR  - 108-2221-E-992-046/Ministry of Science and Technology (MOST), TAIWAN/
GR  - 108-2813-C-992-004-E/Ministry of Science and Technology (MOST), TAIWAN/
PT  - Evaluation Study
PT  - Journal Article
DEP - 20200525
PL  - United States
TA  - J Food Sci
JT  - Journal of food science
JID - 0014052
RN  - 0 (Fatty Acids, Volatile)
SB  - IM
MH  - Animals
MH  - Bacteria/metabolism
MH  - Fatty Acids, Volatile/*blood/metabolism
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Gastrointestinal Microbiome
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - gas chromatography
OT  - rat plasma
OT  - short-chain fatty acid
EDAT- 2020/05/26 06:00
MHDA- 2020/10/27 06:00
CRDT- 2020/05/26 06:00
PHST- 2020/01/19 00:00 [received]
PHST- 2020/04/15 00:00 [revised]
PHST- 2020/04/17 00:00 [accepted]
PHST- 2020/05/26 06:00 [pubmed]
PHST- 2020/10/27 06:00 [medline]
PHST- 2020/05/26 06:00 [entrez]
AID - 10.1111/1750-3841.15172 [doi]
PST - ppublish
SO  - J Food Sci. 2020 Jun;85(6):1932-1938. doi: 10.1111/1750-3841.15172. Epub 2020 May 
      25.