PMID- 32450627
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2233-8233 (Print)
IS  - 2233-8241 (Electronic)
IS  - 2093-8896 (Linking)
VI  - 47
IP  - 2
DP  - 2020 Jun
TI  - Maternal killer-cell immunoglobulin-like receptors and paternal human leukocyte 
      antigen ligands in recurrent pregnancy loss cases in Turkey.
PG  - 122-129
LID - 10.5653/cerm.2019.03223 [doi]
AB  - OBJECTIVE: The survival of a semi-allogeneic fetus depends on several 
      immunological mechanisms, and it has been suggested that recurrent pregnancy loss 
      (RPL) could develop as a result of one or more immunological abnormalities. 
      METHODS: Compatibility between partners for human leukocyte antigen (HLA) 
      genotypes and the relationships between maternal killer-cell immunoglobulin-like 
      receptor (KIR) and paternal HLA-Bw4/Bw6 and HLA-C1/C2 supra-groups were 
      investigated in 25 couples with RPL in comparison to healthy couples with 
      children. HLA and KIR genotyping was performed using polymerase chain reaction 
      with sequence-specific primers and/or sequence-specific oligonucleotides. 
      RESULTS: HLA class I incompatibility between partners, especially in HLA-B 
      alleles, was more common in the RPL group (p= 0.01). HLA-C2 homozygosity was more 
      frequent in the male partners of RPL couples than in other groups (p= 0.03). The 
      KIR2DL5 gene frequency was significantly higher in both the female and male 
      partners of RPL couples, whereas the KIR2DS3 gene frequency in male partners of 
      RPL couples was significantly reduced (p= 0.03). The presence of KIR2DL3 in women 
      with RPL was correlated with the presence of HLA-C2 alleles in their spouses (p= 
      0.03). CONCLUSION: Our data from a Turkish population suggest that male HLA-C2 
      homozygosity may play an important role in RPL. Additionally, an incidental match 
      between male HLA-C2 and female HLA-C1 ligand KIR receptors might perturb the 
      balance between activatory and inhibitory KIR-ligand interactions during 
      pregnancy in couples affected by RPL. The roles of orphan KIR2DL5 and orphan 
      KIR2DS3 in RPL remain obscure.
FAU - Elbasi, Mehmet Onur
AU  - Elbasi MO
AD  - Tissue Typing and Immunology Laboratory, Istanbul Memorial Hospital, Istanbul, 
      Turkey.
FAU - Tulunay, Aysin
AU  - Tulunay A
AD  - Department of Immunology, Marmara University, School of Medicine, Istanbul, 
      Turkey.
FAU - Karagozoglu, Hale
AU  - Karagozoglu H
AD  - Karagozoglu Clinic for Obstetrics and Gynecology and IVF, Istanbul, Turkey.
FAU - Kahraman, Semra
AU  - Kahraman S
AD  - In Vitro Fertilization Unit, Istanbul Memorial Hospital, Istanbul, Turkey.
FAU - Eksioglu-Demiralp, Emel
AU  - Eksioglu-Demiralp E
AD  - Tissue Typing and Immunology Laboratory, Istanbul Memorial Hospital, Istanbul, 
      Turkey.
LA  - eng
GR  - SAG-C-YLP-060308-0035/Marmara Universitesi/
PT  - Journal Article
DEP - 20200526
PL  - Korea (South)
TA  - Clin Exp Reprod Med
JT  - Clinical and experimental reproductive medicine
JID - 101563916
PMC - PMC7315865
OTO - NOTNLM
OT  - HLA C2
OT  - Human leukocyte antigen
OT  - KIR2DL5
OT  - KIR2DS3
OT  - Killer-cell immunoglobulin-like receptor genotyping
OT  - Recurrent pregnancy loss
COIS- Conflict of interest No potential conflict of interest relevant to this article 
      was reported.
EDAT- 2020/05/26 06:00
MHDA- 2020/05/26 06:01
PMCR- 2020/06/01
CRDT- 2020/05/26 06:00
PHST- 2019/08/01 00:00 [received]
PHST- 2020/01/02 00:00 [accepted]
PHST- 2020/05/26 06:00 [pubmed]
PHST- 2020/05/26 06:01 [medline]
PHST- 2020/05/26 06:00 [entrez]
PHST- 2020/06/01 00:00 [pmc-release]
AID - cerm.2019.03223 [pii]
AID - cerm-2019-03223 [pii]
AID - 10.5653/cerm.2019.03223 [doi]
PST - ppublish
SO  - Clin Exp Reprod Med. 2020 Jun;47(2):122-129. doi: 10.5653/cerm.2019.03223. Epub 
      2020 May 26.