PMID- 32451386
OWN - NLM
STAT- MEDLINE
DCOM- 20201201
LR  - 20210525
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 May 25
TI  - Development of type I/II oligodendrocytes regulated by teneurin-4 in the murine 
      spinal cord.
PG  - 8611
LID - 10.1038/s41598-020-65485-0 [doi]
LID - 8611
AB  - In the spinal cord, the axonal tracts with various caliber sizes are myelinated 
      by oligodendrocytes and function as high-velocity ways for motor and sensory 
      nerve signals. In some neurological disorders, such as multiple sclerosis, 
      demyelination of small caliber axons is observed in the spinal cord. While type 
      I/II oligodendrocytes among the four types are known to myelinate small diameter 
      axons, their characteristics including identification of regulating molecules 
      have not been understood yet. Here, we first found that in the wild-type mouse 
      spinal cord, type I/II oligodendrocytes, positive for carbonic anhydrase II 
      (CAII), were located in the corticospinal tract, fasciculus gracilis, and the 
      inside part of ventral funiculus, in which small diameter axons existed. The type 
      I/II oligodendrocytes started to appear between postnatal day (P) 7 and 11. We 
      further analyzed the type I/II oligodendrocytes in the mutant mice, whose small 
      diameter axons were hypomyelinated due to the deficiency of teneurin-4. In the 
      teneurin-4 deficient mice, type I/II oligodendrocytes were significantly reduced, 
      and the onset of the defect was at P11. Our results suggest that CAII-positive 
      type I/II oligodendrocytes myelinate small caliber axons in the spinal cord and 
      teneurin-4 is the responsible molecule for the generation of type I/II 
      oligodendrocytes.
FAU - Hayashi, Chikako
AU  - Hayashi C
AD  - Department of Molecular and Cellular Biology, Graduate School of Medical and 
      Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
AD  - Department of Biochemistry and Biophysics, Graduate School of Health Care 
      Sciences, TMDU, Tokyo, Japan.
FAU - Suzuki, Nobuharu
AU  - Suzuki N
AD  - Department of Molecular and Cellular Biology, Graduate School of Medical and 
      Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan. 
      nsuzbb@tmd.ac.jp.
AD  - Department of Biochemistry and Biophysics, Graduate School of Health Care 
      Sciences, TMDU, Tokyo, Japan. nsuzbb@tmd.ac.jp.
FAU - Takahashi, Riko
AU  - Takahashi R
AD  - Department of Molecular and Cellular Biology, Graduate School of Medical and 
      Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
FAU - Akazawa, Chihiro
AU  - Akazawa C
AD  - Department of Biochemistry and Biophysics, Graduate School of Health Care 
      Sciences, TMDU, Tokyo, Japan. c.akazawa.bb@tmd.ac.jp.
AD  - Department of Biochemistry and Biophysics, Graduate School of Medical and Dental 
      Sciences, TMDU, Tokyo, Japan. c.akazawa.bb@tmd.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200525
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Membrane Proteins)
RN  - 0 (Tenm4 protein, mouse)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Axons/metabolism
MH  - Carbonic Anhydrase II/metabolism
MH  - Intermediate Filaments/metabolism
MH  - Membrane Proteins/deficiency/genetics/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Myelin Sheath/metabolism
MH  - Oligodendroglia/enzymology/*metabolism
MH  - Pyramidal Tracts/metabolism
MH  - Spinal Cord/growth & development/*metabolism
PMC - PMC7248063
COIS- The authors declare no competing interests.
EDAT- 2020/05/27 06:00
MHDA- 2020/12/02 06:00
PMCR- 2020/05/25
CRDT- 2020/05/27 06:00
PHST- 2019/11/03 00:00 [received]
PHST- 2020/04/29 00:00 [accepted]
PHST- 2020/05/27 06:00 [entrez]
PHST- 2020/05/27 06:00 [pubmed]
PHST- 2020/12/02 06:00 [medline]
PHST- 2020/05/25 00:00 [pmc-release]
AID - 10.1038/s41598-020-65485-0 [pii]
AID - 65485 [pii]
AID - 10.1038/s41598-020-65485-0 [doi]
PST - epublish
SO  - Sci Rep. 2020 May 25;10(1):8611. doi: 10.1038/s41598-020-65485-0.