PMID- 32452516
OWN - NLM
STAT- MEDLINE
DCOM- 20210323
LR  - 20210323
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 40
IP  - 6
DP  - 2020 Jun 26
TI  - Vitamin D-vitamin D receptor system down-regulates expression of uncoupling 
      proteins in brown adipocyte through interaction with Hairless protein.
LID - 10.1042/BSR20194294 [doi]
LID - BSR20194294
AB  - Our previous study showed that feeding mice with vitamin D deficiency diet 
      markedly alleviated high-fat-diet-induced overweight, hyperinsulinemia, and 
      hepatic lipid accumulation. Moreover, vitamin D deficiency up-regulated the 
      expression of uncoupling protein 3 (Ucp3) in white adipose tissue (WAT) and brown 
      adipose tissue (BAT). The present study aimed to further investigate the effects 
      of vitamin D and vitamin D receptor (Vdr) on Ucp1-3 (Ucps) expression in brown 
      adipocyte and the mechanism involved in it. Rat primary brown adipocytes were 
      separated and purified. The effects of the 1,25(OH)2D3 (1,25-dihydroxyvitamin D3; 
      the hormonal form of vitamin D) and Vdr system on Ucps expression in brown 
      adipocytes were investigated in basal condition and activated condition by 
      isoproterenol (ISO) and triiodothyronine (T3). Ucps expression levels were 
      significantly down-regulated by 1,25(OH)2D3 in the activated brown adipocyte. Vdr 
      silencing reversed the down-regulation of Ucps by 1,25(OH)2D3, whereas Vdr 
      overexpression strengthened the down-regulation effects. Hairless protein did 
      express in brown adipocyte and was localized in cell nuclei. 1,25(OH)2D3 
      increased Hairless protein expression in the cell nuclei. Hairless (Hr) silencing 
      notably elevated Ucps expression in activated condition induced by ISO and T3. 
      Moreover, immunoprecipitation results revealed that Vdr could interact with 
      Hairless, which might contribute to decreasing expression of Vdr target gene 
      Ucps. These data suggest that vitamin D suppresses expression of Ucps in brown 
      adipocyte in a Vdr-dependent manner and the corepressor Hairless protein probably 
      plays a role in the down-regulation.
CI  - (c) 2020 The Author(s).
FAU - Wang, Pei-Qi
AU  - Wang PQ
AD  - Department of Pediatrics, First Affiliated Hospital, Anhui Medical University, 
      218 Jixi Road, Hefei 230 022, Anhui Province, China.
FAU - Pan, Dao-Xiang
AU  - Pan DX
AD  - Department of Pediatrics, First Affiliated Hospital, Anhui Medical University, 
      218 Jixi Road, Hefei 230 022, Anhui Province, China.
FAU - Hu, Chun-Qiu
AU  - Hu CQ
AD  - Department of Toxicology, Anhui Medical University, 81 Meishan Road, Hefei 230 
      032, Anhui Province, China.
FAU - Zhu, Yu-Lin
AU  - Zhu YL
AD  - Department of Pediatrics, First Affiliated Hospital, Anhui Medical University, 
      218 Jixi Road, Hefei 230 022, Anhui Province, China.
FAU - Liu, Xiao-Jing
AU  - Liu XJ
AD  - Department of Pediatrics, First Affiliated Hospital, Anhui Medical University, 
      218 Jixi Road, Hefei 230 022, Anhui Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Mitochondrial Uncoupling Proteins)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Transcription Factors)
RN  - 0 (Ucp1 protein, rat)
RN  - 0 (Ucp2 protein, rat)
RN  - 0 (Ucp3 protein, rat)
RN  - 0 (Uncoupling Protein 1)
RN  - 0 (Uncoupling Protein 2)
RN  - 0 (Uncoupling Protein 3)
RN  - 0 (Vitamins)
RN  - 0 (hr protein, rat)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Adipocytes, Brown/*drug effects/metabolism
MH  - Animals
MH  - Calcitriol/*pharmacology
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Male
MH  - Mitochondrial Uncoupling Proteins/genetics/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Receptors, Calcitriol/*agonists/genetics/metabolism
MH  - Signal Transduction
MH  - Transcription Factors/genetics/*metabolism
MH  - Uncoupling Protein 1/genetics/metabolism
MH  - Uncoupling Protein 2/genetics/metabolism
MH  - Uncoupling Protein 3/genetics/metabolism
MH  - Vitamins/*pharmacology
PMC - PMC7286880
OTO - NOTNLM
OT  - 1,25(OH)2D3
OT  - hairless protein
OT  - uncoupling proteins
OT  - vitamin D
OT  - vitamin D receptor
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2020/05/27 06:00
MHDA- 2021/03/24 06:00
PMCR- 2020/06/10
CRDT- 2020/05/27 06:00
PHST- 2019/12/30 00:00 [received]
PHST- 2020/05/13 00:00 [revised]
PHST- 2020/05/14 00:00 [accepted]
PHST- 2020/05/27 06:00 [pubmed]
PHST- 2021/03/24 06:00 [medline]
PHST- 2020/05/27 06:00 [entrez]
PHST- 2020/06/10 00:00 [pmc-release]
AID - 225002 [pii]
AID - BSR20194294 [pii]
AID - 10.1042/BSR20194294 [doi]
PST - ppublish
SO  - Biosci Rep. 2020 Jun 26;40(6):BSR20194294. doi: 10.1042/BSR20194294.