PMID- 32452520
OWN - NLM
STAT- MEDLINE
DCOM- 20200904
LR  - 20210526
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 98
IP  - 6
DP  - 2020 Jun 1
TI  - Technical note: Relationship between placentome location and gene expression in 
      bovine pregnancy.
LID - 10.1093/jas/skaa176 [doi]
LID - skaa176
AB  - A novel, non-terminal surgical procedure to remove a single placentome from the 
      pregnant ewe for gene expression and histological analyses was recently developed 
      in our laboratory. This technique allows for evaluation of nutritional insults on 
      placental development at more than one stage of gestation using a single animal. 
      Early attempts to develop a similar technique in cattle were met with 
      complications due to inaccessibility of the gravid uterine horn because of its 
      location and mass. One alternative is to collect a placentome from the 
      contralateral uterine horn; however, the question remains as to whether gene 
      expression varies among placentomes based on location relative to the fetus. 
      Pregnant heifers were maintained on forage during early gestation and later moved 
      into pens with a Calan gate system (American Calan, Northwood, NH). On 
      gestational day (GD) 158, five heifers were assigned to receive a hay-based diet 
      formulated to meet 100% of NRC requirements, and five heifers were fed 70% of NRC 
      requirements until necropsy on GD244. At necropsy, a single representative 
      placentome was selected for analysis from the antimesometrial side: (1) of the 
      gravid uterine horn central to the amnion, (2) over the allantois immediately 
      adjacent to the amnion, (3) in the tip of the gravid uterine horn, and (4) in the 
      tip of the contralateral uterine horn. Mean placentome weight was greater (P < 
      0.05) for locations central to the amnion and allantois compared to locations 
      within the tips of the ipsilateral and contralateral horns, respectively. Gene 
      expression for angiogenic factors (FGF2, ODC1, VEGFA, and FLT1), nutrient 
      transporters (SLC7A1 and SLC2A1), and factors associated with hormone action 
      (ESR1, IGF1, IGFBP3, CSH1, and PAG1) were unaffected (P > 0.05) by dietary 
      treatment or location of the placentome. Results indicate that location of the 
      placentome in relation to the fetus does not impact gene expression, enhancing 
      the efficacy of nonterminal methodologies for sampling gene expression in 
      placentomes.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of the 
      American Society of Animal Science. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Picking, Elizabeth M
AU  - Picking EM
AD  - Department of Animal Science, Texas A&M University, College Station, TX.
FAU - Trubenbach, Levi A
AU  - Trubenbach LA
AD  - Department of Animal Science, Texas A&M University, College Station, TX.
FAU - Bazer, Fuller W
AU  - Bazer FW
AD  - Department of Animal Science, Texas A&M University, College Station, TX.
FAU - Sawyer, Jason E
AU  - Sawyer JE
AD  - Department of Animal Science, Texas A&M University, College Station, TX.
AD  - King Ranch(R) Institute for Ranch Management, Texas A&M University, Kingsville, TX.
FAU - Wickersham, Tryon A
AU  - Wickersham TA
AD  - Department of Animal Science, Texas A&M University, College Station, TX.
FAU - Satterfield, M Carey
AU  - Satterfield MC
AD  - Department of Animal Science, Texas A&M University, College Station, TX.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
SB  - IM
MH  - Animals
MH  - Cattle/*physiology
MH  - Diet/veterinary
MH  - Female
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Nutrients
MH  - Placenta/*metabolism
MH  - Placentation
MH  - Pregnancy
MH  - *Pregnancy, Animal
MH  - Uterus/*physiology
PMC - PMC7276672
OTO - NOTNLM
OT  - bovine
OT  - gene expression
OT  - nutrient restriction
OT  - placentome
EDAT- 2020/05/27 06:00
MHDA- 2020/09/05 06:00
PMCR- 2021/05/25
CRDT- 2020/05/27 06:00
PHST- 2020/04/07 00:00 [received]
PHST- 2020/05/18 00:00 [accepted]
PHST- 2020/05/27 06:00 [pubmed]
PHST- 2020/09/05 06:00 [medline]
PHST- 2020/05/27 06:00 [entrez]
PHST- 2021/05/25 00:00 [pmc-release]
AID - 5844073 [pii]
AID - skaa176 [pii]
AID - 10.1093/jas/skaa176 [doi]
PST - ppublish
SO  - J Anim Sci. 2020 Jun 1;98(6):skaa176. doi: 10.1093/jas/skaa176.