PMID- 32454462
OWN - NLM
STAT- MEDLINE
DCOM- 20210219
LR  - 20210219
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 12
IP  - 10
DP  - 2020 May 26
TI  - MiR-1908/EXO1 and MiR-203a/FOS, regulated by scd1, are associated with fracture 
      risk and bone health in postmenopausal diabetic women.
PG  - 9549-9584
LID - 10.18632/aging.103227 [doi]
AB  - BACKGROUND: Stearoyl-coenzyme A desaturase-1 (SCD1) can inhibit the development 
      of diabetic bone disease by promoting osteogenesis. In this study, we examined 
      whether this regulation by SCD1 is achieved by regulating the expression of 
      related miRNAs. METHODS: SCD1 expression levels were observed in human 
      bone-marrow mesenchymal stem cells (BM-MSCs) of patients with type 2 diabetes 
      mellitus (T2DM), and the effect of SCD1 on osteogenesis was observed in human 
      adipose-derived MSCs transfected with the SCD1 lentiviral system. We designed a 
      bioinformatics prediction model to select important differentially expressed 
      miRNAs, and established protein-protein interaction and miRNA-mRNA networks. 
      miRNAs and mRNAs were extracted and their differential expression was detected. 
      The SCD1-miRNA-mRNA network was validated. FINDINGS: SCD1 expression in bone 
      marrow was downregulated in patients with T2DM and low-energy fracture, and SCD1 
      expression promotes BM-MSC osteogenic differentiation. The predictors in the 
      nomogram were seven microRNAs, including hsa-miR-1908 and hsa-miR-203a. SCD1 
      inhibited the expression of CDKN1A and FOS, but promoted the expression of EXO1 
      and PLS1. miR-1908 was a regulator of EXO1 expression, and miR-203a was a 
      regulator of FOS expression. INTERPRETATION: The regulation of BM-MSCs by SCD1 is 
      a necessary condition for osteogenesis through the miR-203a/FOS and miR-1908/EXO1 
      regulatory pathways.
FAU - Chen, Yi-Sheng
AU  - Chen YS
AD  - Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
FAU - Kang, Xue-Ran
AU  - Kang XR
AD  - Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's 
      Hospital, Shanghai Jiao Tong University School of Medicine, Ear Institute, 
      Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
FAU - Zhou, Zi-Hui
AU  - Zhou ZH
AD  - Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
FAU - Yang, Jiang
AU  - Yang J
AD  - Department of Neurosurgery, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
FAU - Xin, Qi
AU  - Xin Q
AD  - Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
FAU - Ying, Chen-Ting
AU  - Ying CT
AD  - Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
FAU - Zhang, Yun-Peng
AU  - Zhang YP
AD  - Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
FAU - Tao, Jie
AU  - Tao J
AD  - Department of Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200526
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (FOS protein, human)
RN  - 0 (Genetic Markers)
RN  - 0 (MIRN1908 microRNA, human)
RN  - 0 (MIRN203 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - EC 1.14.19.1 (SCD1 protein, human)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
RN  - EC 3.1.- (EXO1 protein, human)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - Cyclin-Dependent Kinase Inhibitor p21/metabolism
MH  - DNA Repair Enzymes/metabolism
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Down-Regulation/genetics
MH  - Exodeoxyribonucleases/metabolism
MH  - Female
MH  - Fractures, Bone/*genetics
MH  - Genetic Markers/genetics
MH  - Humans
MH  - Mesenchymal Stem Cells/metabolism
MH  - MicroRNAs/*metabolism
MH  - Nomograms
MH  - Postmenopause/*genetics
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Risk Assessment/methods
MH  - Risk Factors
MH  - Stearoyl-CoA Desaturase/*metabolism
PMC - PMC7288911
OTO - NOTNLM
OT  - bone mesenchymal stem cell (BMSC)
OT  - diabetes
OT  - fracture risk
OT  - nomogram
OT  - stearoyl-coenzyme A desaturase (SCD1)
COIS- CONFLICTS OF INTEREST: The authors declare that they have no conflicts of 
      interest.
EDAT- 2020/05/27 06:00
MHDA- 2021/02/20 06:00
PMCR- 2020/05/31
CRDT- 2020/05/27 06:00
PHST- 2019/09/03 00:00 [received]
PHST- 2020/04/13 00:00 [accepted]
PHST- 2020/05/27 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/05/27 06:00 [entrez]
PHST- 2020/05/31 00:00 [pmc-release]
AID - 103227 [pii]
AID - 10.18632/aging.103227 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2020 May 26;12(10):9549-9584. doi: 10.18632/aging.103227. Epub 
      2020 May 26.