PMID- 32455871
OWN - NLM
STAT- MEDLINE
DCOM- 20210222
LR  - 20210222
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 12
IP  - 5
DP  - 2020 May 22
TI  - Genome Analysis of a Novel Tembusu Virus in Taiwan.
LID - 10.3390/v12050567 [doi]
LID - 567
AB  - We identified and isolated a novel Tembusu virus (TMUV) strain TP1906 
      (TMUV-TP1906) from a Culexannulus mosquito pool collected from the northern part 
      of Taiwan in 2019. The TMUV-TP1906 genome is a 10,990-nucleotide-long, 
      positive-sense, single-stranded RNA, consisting of a single open reading frame 
      (ORF) encoding a polyprotein of 3425 amino acids, with 5' and 3' untranslated 
      regions (UTRs) of 94 and 618 nucleotides, respectively. The nucleotide sequence 
      of the TMUV-TP1906 of ORF exhibited 93.71% and 91.27% similarity with Sitiawan 
      virus (STWV) and the TMUV prototype strain MM1775, respectively. The 3'-UTR 
      variable region of TMUV-TP1906 showed nucleotide sequence divergence with other 
      TMUV strains. Phylogenetic analysis of the complete ORF and polyprotein sequences 
      revealed that TMUV-TP1906 is most closely related to STWV which causes 
      encephalitis and retarded growth in chickens. We found that the TMUV-TP1906 
      caused a cytopathic effect (CPE) in the DF-1 chicken fibroblast cell line, while 
      no apparent CPE was observed in Vero and C6/36 cells. In this study, we first 
      identified and isolated a novel TMUV strain in Taiwan. In addition, to our 
      knowledge, it is the first time that the TMUV strain was isolated from the Cx. 
      annulus mosquitoes. Further study is warranted to investigate the host range and 
      virulence of TMUV-TP1906.
FAU - Peng, Shih-Huan
AU  - Peng SH
AUID- ORCID: 0000-0002-7540-6027
AD  - Center for Diagnostics and Vaccine Development, Centers for Disease Control, 
      Ministry of Health and Welfare, Taipei City 11561, Taiwan.
FAU - Su, Chien-Ling
AU  - Su CL
AD  - Center for Diagnostics and Vaccine Development, Centers for Disease Control, 
      Ministry of Health and Welfare, Taipei City 11561, Taiwan.
FAU - Chang, Mei-Chun
AU  - Chang MC
AD  - Center for Diagnostics and Vaccine Development, Centers for Disease Control, 
      Ministry of Health and Welfare, Taipei City 11561, Taiwan.
FAU - Hu, Huai-Chin
AU  - Hu HC
AD  - Center for Diagnostics and Vaccine Development, Centers for Disease Control, 
      Ministry of Health and Welfare, Taipei City 11561, Taiwan.
FAU - Yang, Su-Lin
AU  - Yang SL
AD  - Center for Diagnostics and Vaccine Development, Centers for Disease Control, 
      Ministry of Health and Welfare, Taipei City 11561, Taiwan.
FAU - Shu, Pei-Yun
AU  - Shu PY
AUID- ORCID: 0000-0002-1670-9749
AD  - Center for Diagnostics and Vaccine Development, Centers for Disease Control, 
      Ministry of Health and Welfare, Taipei City 11561, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200522
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Polyproteins)
RN  - Tembusu virus
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chickens
MH  - Chlorocebus aethiops
MH  - Culicidae/virology
MH  - Fibroblasts
MH  - Flavivirus/*classification/*genetics/growth & development/*isolation & 
      purification
MH  - Flavivirus Infections/veterinary/virology
MH  - *Genome, Viral
MH  - Host Specificity
MH  - Kinetics
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Polyproteins/genetics
MH  - Poultry Diseases/virology
MH  - Taiwan
MH  - Vero Cells
MH  - Whole Genome Sequencing
PMC - PMC7290467
OTO - NOTNLM
OT  - Sitiawan virus
OT  - Tembusu virus
OT  - flavivirus 3'-UTR variable region
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results
EDAT- 2020/05/28 06:00
MHDA- 2021/02/23 06:00
PMCR- 2020/05/01
CRDT- 2020/05/28 06:00
PHST- 2020/04/07 00:00 [received]
PHST- 2020/05/20 00:00 [revised]
PHST- 2020/05/20 00:00 [accepted]
PHST- 2020/05/28 06:00 [entrez]
PHST- 2020/05/28 06:00 [pubmed]
PHST- 2021/02/23 06:00 [medline]
PHST- 2020/05/01 00:00 [pmc-release]
AID - v12050567 [pii]
AID - viruses-12-00567 [pii]
AID - 10.3390/v12050567 [doi]
PST - epublish
SO  - Viruses. 2020 May 22;12(5):567. doi: 10.3390/v12050567.