PMID- 32458505
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 49
IP  - 2
DP  - 2020 Jun
TI  - Spurious, marked leukocytosis in 2 cats with Heinz body hemolytic anemia.
PG  - 232-239
LID - 10.1111/vcp.12860 [doi]
AB  - Two domestic shorthair cats were presented with anorexia and dehydration 
      following ingestion of caramelized onions. Shared key findings from a CBC (ADVIA 
      2120), serum biochemistry, and urinalysis included a spurious, marked 
      leukocytosis with discordant basophil (BASO) channel and peroxidase channel WBC 
      counts, normal manual leukocyte counts, mild, non-regenerative anemia with 
      discrepancies between automated and manual reticulocyte counts, an abundance of 
      large Heinz bodies (HBs), and highly irregular scattergrams. Case 1 also 
      demonstrated a markedly elevated mean corpuscular hemoglobin concentration (MCHC) 
      and discrepancies between RBC hemoglobin indices. Spurious leukocyte results were 
      confirmed through re-analysis of samples (including the acquisition of a new 
      sample, use of an alternate analyzer (Sysmex XT-2000iV; Case 1 only), and 
      evaluation of scattergrams and blood films (Cases 1 and 2). Repeatedly discrepant 
      reticulocyte counts were also identified. In both cases, the erroneous BASO WBC 
      counts, discrepancies in reticulocyte counts and RBC indices, and atypical 
      scattergrams were interpreted to result from various effects of the HBs. These 
      cases emphasize the importance of reviewing blood films, interpreting 
      scattergrams, and the usefulness of duplicate methods for determining various 
      measurands on hematology analyzers.
CI  - (c) 2020 American Society for Veterinary Clinical Pathology.
FAU - Johnson, Courtney E
AU  - Johnson CE
AUID- ORCID: 0000-0001-7534-4981
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, MN, USA.
FAU - Seelig, Davis M
AU  - Seelig DM
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, MN, USA.
FAU - Moore, Frances M
AU  - Moore FM
AD  - Veterinary Services, Marshfield Labs, Marshfield, WI, USA.
FAU - Ruska, Tammy J
AU  - Ruska TJ
AD  - Veterinary Medical Center, University of Minnesota, St. Paul, MN, USA.
FAU - Heinrich, Daniel A
AU  - Heinrich DA
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, MN, USA.
LA  - eng
PT  - Case Reports
DEP - 20200526
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
SB  - IM
MH  - Anemia, Hemolytic/blood/diagnosis/pathology/*veterinary
MH  - Animals
MH  - Basophils/pathology
MH  - Cat Diseases/blood/*diagnosis/pathology
MH  - Cats
MH  - Female
MH  - Heinz Bodies/pathology
MH  - Hematology/instrumentation
MH  - Hemolysis
MH  - Leukocyte Count/veterinary
MH  - Leukocytosis/blood/diagnosis/pathology/*veterinary
MH  - Male
MH  - Oxidative Stress
MH  - Reticulocyte Count/veterinary
MH  - Urinalysis/veterinary
OTO - NOTNLM
OT  - ADVIA
OT  - Sysmex
OT  - feline
OT  - hemolysis
OT  - oxidative damage
EDAT- 2020/05/28 06:00
MHDA- 2021/03/30 06:00
CRDT- 2020/05/28 06:00
PHST- 2019/08/23 00:00 [received]
PHST- 2019/10/18 00:00 [revised]
PHST- 2019/10/21 00:00 [accepted]
PHST- 2020/05/28 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/05/28 06:00 [entrez]
AID - 10.1111/vcp.12860 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2020 Jun;49(2):232-239. doi: 10.1111/vcp.12860. Epub 2020 May 
      26.