PMID- 32460017
OWN - NLM
STAT- MEDLINE
DCOM- 20210521
LR  - 20210521
IS  - 2211-1247 (Electronic)
VI  - 31
IP  - 8
DP  - 2020 May 26
TI  - Pyruvate Kinase M2 Promotes the Activation of Dendritic Cells by Enhancing 
      IL-12p35 Expression.
PG  - 107690
LID - S2211-1247(20)30643-4 [pii]
LID - 10.1016/j.celrep.2020.107690 [doi]
AB  - Dendritic cells (DCs) play a central role in both innate and adaptive immunity. 
      Emerging evidence has demonstrated metabolic reprogramming during DC activation. 
      However, how DC activation is linked with metabolic reprogramming remains 
      unclear. Here we show that pyruvate kinase M2 (PKM2), the rate-limiting enzyme in 
      the last step of glycolysis, is critical for LPS-induced DC activation. Upon DC 
      activation, JNK signaling stimulated p300 association with PKM2 for the 
      acetylation of lysine 433, a classic posttranslational modification critical for 
      PKM2 destabilization and nuclear re-localization. Subsequently, nuclear PKM2 
      partnered with c-Rel to enhance Il12p35 expression, which is important for Th1 
      cell differentiation. Meanwhile, decreased enzymatic activity of PKM2 due to 
      detetramerization facilitated glycolysis and fatty acid synthesis, helping DCs 
      meet their need for biomacromolecules. Together, we provide evidence for 
      metabolic control of DC activation and offer insights into aberrant immune 
      responses due to dysregulated Th1 functions.
CI  - Copyright (c) 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Jin, Xin
AU  - Jin X
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Zhang, Wenxia
AU  - Zhang W
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Liu, Jia
AU  - Liu J
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Hao, Fengqi
AU  - Hao F
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Li, Yunlong
AU  - Li Y
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Tian, Miaomiao
AU  - Tian M
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Shu, Hengyao
AU  - Shu H
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Dong, Jiaxin
AU  - Dong J
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China.
FAU - Feng, Yunpeng
AU  - Feng Y
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China. Electronic address: fengyp0108@nenu.edu.cn.
FAU - Wei, Min
AU  - Wei M
AD  - Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), 
      Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, 
      People's Republic of China. Electronic address: weim750@nenu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Interleukin-12 Subunit p35)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Dendritic Cells/*metabolism
MH  - Humans
MH  - Interleukin-12 Subunit p35/*metabolism
MH  - Pyruvate Kinase/*metabolism
OTO - NOTNLM
OT  - IL-12
OT  - JNK
OT  - K433 acetylation
OT  - PKM2
OT  - dendritic cell
COIS- Declaration of Interests The authors declare no competing interests.
EDAT- 2020/05/28 06:00
MHDA- 2021/05/22 06:00
CRDT- 2020/05/28 06:00
PHST- 2019/10/10 00:00 [received]
PHST- 2020/03/06 00:00 [revised]
PHST- 2020/05/01 00:00 [accepted]
PHST- 2020/05/28 06:00 [entrez]
PHST- 2020/05/28 06:00 [pubmed]
PHST- 2021/05/22 06:00 [medline]
AID - S2211-1247(20)30643-4 [pii]
AID - 10.1016/j.celrep.2020.107690 [doi]
PST - ppublish
SO  - Cell Rep. 2020 May 26;31(8):107690. doi: 10.1016/j.celrep.2020.107690.