PMID- 32460585
OWN - NLM
STAT- MEDLINE
DCOM- 20210716
LR  - 20210716
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Linking)
VI  - 40
IP  - 7
DP  - 2020 Jul
TI  - Inverse Relationship Between Lipopolysaccharide Concentration and Monocyte and 
      Dendritic Cells Inflammatory Response.
PG  - 349-356
LID - 10.1089/jir.2019.0244 [doi]
AB  - Dendritic cells (DCs) and monocytes are key immunoregulatory cells that link the 
      innate and adaptive immune response. However, understanding of human 
      cell-specific responses to different doses of stimuli including 
      lipopolysaccharide (LPS) is limited. This study investigated the monocyte and 
      classical DC (cDC)-specific, as well as the overall inflammatory response after 
      exposure to varying doses of LPS. Fresh peripheral whole blood (n = 8) was used 
      in an in vitro peripheral blood culture model to assess cDC and monocyte 
      responses in coculture with varying doses of LPS (0.25, 0.5, 0.75, 1 mug/mL). cDC 
      and monocyte cytokine responses were measured through flow cytometry. 
      Supernatants collected from the in vitro model were used in a cytometric bead 
      array to assess the overall inflammatory response. Exposure to all doses of LPS 
      tested increased monocyte, cDC, and the overall leukocyte response. A 
      dose-dependent reduction in cDC and monocyte cytokine production was also evident 
      with higher LPS doses. This study demonstrates that cell-subset-specific 
      responses are more susceptible to LPS exposure compared with the overall 
      inflammatory response. Therefore, assays that assess cell-specific immune 
      responses may be more beneficial to identify underlying pathophysiology of 
      infection and inflammation.
FAU - Perros, Alexis J
AU  - Perros AJ
AD  - Research and Development, Australian Red Cross Lifeblood, Brisbane, Queensland, 
      Australia.
AD  - Faculty of Medicine, University of Queensland, Brisbane, Queensland, Australia.
AD  - Faculty of Health, Queensland University of Technology, Brisbane, Queensland, 
      Australia.
FAU - Flower, Robert L
AU  - Flower RL
AD  - Research and Development, Australian Red Cross Lifeblood, Brisbane, Queensland, 
      Australia.
AD  - Faculty of Health, Queensland University of Technology, Brisbane, Queensland, 
      Australia.
FAU - Dean, Melinda M
AU  - Dean MM
AD  - Research and Development, Australian Red Cross Lifeblood, Brisbane, Queensland, 
      Australia.
AD  - Faculty of Health, Queensland University of Technology, Brisbane, Queensland, 
      Australia.
AD  - School of Health and Sport Sciences, University of the Sunshine Coast, Petrie, 
      Queensland, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200527
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Cells, Cultured
MH  - Cytokines/*biosynthesis/blood/immunology
MH  - Dendritic Cells/*drug effects/immunology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Inflammation/*immunology
MH  - Lipopolysaccharides/*pharmacology
MH  - Monocytes/*drug effects/immunology
OTO - NOTNLM
OT  - dendritic cells (DCs)
OT  - endotoxin tolerance
OT  - leukocyte response
OT  - lipopolysaccharide
OT  - monocytes
EDAT- 2020/05/29 06:00
MHDA- 2021/07/17 06:00
CRDT- 2020/05/29 06:00
PHST- 2020/05/29 06:00 [pubmed]
PHST- 2021/07/17 06:00 [medline]
PHST- 2020/05/29 06:00 [entrez]
AID - 10.1089/jir.2019.0244 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2020 Jul;40(7):349-356. doi: 10.1089/jir.2019.0244. 
      Epub 2020 May 27.