PMID- 32462240
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 1573-6881 (Electronic)
IS  - 0145-479X (Linking)
VI  - 52
IP  - 4
DP  - 2020 Aug
TI  - Functional changes induced by caloric restriction in cardiac and skeletal muscle 
      mitochondria.
PG  - 269-277
LID - 10.1007/s10863-020-09838-4 [doi]
AB  - Caloric restriction (CR) is widely known to increase life span and resistance to 
      different types of injuries in several organisms. We have previously shown that 
      mitochondria from livers or brains of CR animals exhibit higher calcium uptake 
      rates and lower sensitivity to calcium-induced mitochondrial permeability 
      transition (mPT), an event related to the resilient phenotype exhibited by these 
      organs. Given the importance of calcium in metabolic control and cell 
      homeostasis, we aimed here to uncover possible changes in mitochondrial calcium 
      handling, redox balance and bioenergetics in cardiac and skeletal muscle 
      mitochondria in response to six months of CR. Unexpectedly, we found that CR does 
      not alter the susceptibility to mPT in muscle (cardiac or skeletal), nor calcium 
      uptake rates. Despite the lack in changes in calcium transport properties, CR 
      consistently decreased respiration in the presence of ATP synthesis in heart and 
      soleus muscle. In heart, such changes were accompanied by a decrease in 
      respiration in the absence of ATP synthesis, lower maximal respiratory rates and 
      a reduced rate of hydrogen peroxide release. Hydrogen peroxide release was 
      unaltered by CR in skeletal muscle. No changes were observed in inner membrane 
      potentials and respiratory control ratios. Together, these results highlight the 
      tissue-specific bioenergetic and ion transport effects induced by CR, 
      demonstrating that resilience against calcium-induced mPT is not present in all 
      tissues.
FAU - Serna, Julian David C
AU  - Serna JDC
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, Brazil.
FAU - Caldeira da Silva, Camille C
AU  - Caldeira da Silva CC
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, Brazil.
FAU - Kowaltowski, Alicia J
AU  - Kowaltowski AJ
AD  - Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao 
      Paulo, Brazil. alicia@iq.usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200527
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
SB  - IM
MH  - Animals
MH  - Caloric Restriction/*standards
MH  - Energy Metabolism/*physiology
MH  - Male
MH  - Muscle, Skeletal/*immunology
MH  - Myocardium/*immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Bioenergetics
OT  - Caloric Restriction
OT  - Heart
OT  - Mitochondrial Permeability Transition
OT  - Reactive Oxygen Species
OT  - Soleus Muscle
EDAT- 2020/05/29 06:00
MHDA- 2021/06/25 06:00
CRDT- 2020/05/29 06:00
PHST- 2020/03/16 00:00 [received]
PHST- 2020/05/22 00:00 [accepted]
PHST- 2020/05/29 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
PHST- 2020/05/29 06:00 [entrez]
AID - 10.1007/s10863-020-09838-4 [pii]
AID - 10.1007/s10863-020-09838-4 [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2020 Aug;52(4):269-277. doi: 10.1007/s10863-020-09838-4. 
      Epub 2020 May 27.