PMID- 32467785
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2168-8184 (Print)
IS  - 2168-8184 (Electronic)
IS  - 2168-8184 (Linking)
VI  - 12
IP  - 4
DP  - 2020 Apr 24
TI  - Evaluation of C-C Chemokine Ligand 5 (CCL5) Chemokine, Interleukin 5 (IL-5) 
      Cytokine, and Eosinophil Counts as Potential Biomarkers in Saudi Patients with 
      Chronic Asthma During Sandstorms.
PG  - e7809
LID - 10.7759/cureus.7809 [doi]
LID - e7809
AB  - Background and objectives Asthma is a common chronic inflammatory disorder of the 
      lung that can be exacerbated by environmental triggers during sandstorms. This 
      study aimed to evaluate the usefulness of C-C chemokine ligand 5 (CCL5) chemokine 
      and interleukin 5 (IL-5) cytokine and determine the total eosinophil count in 
      blood and sputum for use as biomarkers in Saudi patients with chronic asthma who 
      visited emergency departments during sandstorms. Methods The study included 42 
      Saudi patients with chronic asthma and 20 healthy controls. Plasma levels of 
      CCL5, IL-5, and total immunoglobulin E (IgE) were measured using a specific 
      enzyme-linked immunosorbent assay (ELISA). Total eosinophils in peripheral blood 
      were counted using a hematology analyzer (CELL-DYN Ruby System; Abbott 
      Diagnostics, Chicago, Illinois); in sputum, eosinophils stained with Giemsa were 
      examined under a microscope, counted, and expressed as a percentage of the total 
      cells. Results Total IgE levels were significantly higher in patients with asthma 
      (mean 433 IU/ml, P = 0.0001) as compared to normal controls (139 IU/ml). There 
      was no significant difference in the levels of CCL5 in patients with asthma (625 
      pg/ml) as compared to normal controls (663 pg/ml, P = 0. 57). No correlation was 
      found between total IgE and CCL5 levels. IL-5 was not detected in patients with 
      asthma or in controls. Moreover, the total counts of eosinophils in the blood did 
      not increase in patients with asthma as compared to controls while eosinophils in 
      sputum samples were increased in the former (mean =3.128%). Conclusion Plasma 
      levels of CCL5 and IL-5 or eosinophil counts in the peripheral blood may not be 
      useful diagnostic biomarkers to evaluate airway inflammation and monitor asthma 
      severity. Conversely, the sputum eosinophil count may represent a useful 
      diagnostic marker for assessing the magnitude of asthma exacerbation during 
      sandstorms.
CI  - Copyright (c) 2020, Alturaiki et al.
FAU - Alturaiki, Wael H
AU  - Alturaiki WH
AD  - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, 
      Majmaah University, Majmaah, SAU.
LA  - eng
PT  - Journal Article
DEP - 20200424
PL  - United States
TA  - Cureus
JT  - Cureus
JID - 101596737
PMC - PMC7249775
OTO - NOTNLM
OT  - asthma
OT  - ccl5
OT  - eosinophil
OT  - il-5
OT  - sandstorms
OT  - total ige
COIS- The authors have declared that no competing interests exist.
EDAT- 2020/05/30 06:00
MHDA- 2020/05/30 06:01
PMCR- 2020/04/24
CRDT- 2020/05/30 06:00
PHST- 2020/05/30 06:00 [entrez]
PHST- 2020/05/30 06:00 [pubmed]
PHST- 2020/05/30 06:01 [medline]
PHST- 2020/04/24 00:00 [pmc-release]
AID - 10.7759/cureus.7809 [doi]
PST - epublish
SO  - Cureus. 2020 Apr 24;12(4):e7809. doi: 10.7759/cureus.7809.