PMID- 32468480
OWN - NLM
STAT- MEDLINE
DCOM- 20200911
LR  - 20210312
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 1195
DP  - 2020
TI  - Ocimum Sanctum Linn: A Potential Adjunct Therapy for Hyperhomocysteinemia-Induced 
      Vascular Dementia.
PG  - 213-225
LID - 10.1007/978-3-030-32633-3_30 [doi]
AB  - Vascular dementia (VaD) is well recognized as the second most familiar form of 
      dementia in the aged population. The present study is aimed to investigate the 
      neuroprotective effects of ethanolic extract of leaves of Ocimum sanctum (EEOS) 
      against hyperhomocysteinemia (HHcy)-induced vascular dementia (VaD) in Wistar 
      rats. HHcy was induced by administering L-methionine (1.7 g/kg, p.o) for 4 weeks. 
      Donepezil (0.1 mg/kg, p.o.) and EEOS (100 mg/kg, 200 mg/kg, 400 mg/kg, p.o.) were 
      administered from the 14(th) day of L-methionine treatment. The behavioral 
      impairment caused due to HHcy in rats was assessed by the Morris water maze (MWM) 
      and Y-maze tests using a video tracking system. Biochemical estimations and 
      aortic ring assay were also performed followed by a molecular docking analysis of 
      active chemical constituents present in the leaves of Ocimum sanctum Linn. In 
      this study, the EEOS treatment in hyperhomocysteinemic rats has showed 
      significant improvement in spatial learning and working memory performance. The 
      EEOS treatment further increased nitric oxide bioavailability and significantly 
      altered all serum and brain biochemical parameters in a dose-dependent manner. 
      The docking analysis revealed that among all the phytoconstituents of Ocimum 
      sanctum compound (IX), molludistin has showed good inhibitory activity against 
      S-adenosyl homocysteine, thus preventing homocysteine formation and may be 
      responsible for potential effects of EEOS against HHcy-induced VaD. From our 
      results, we conclude that EEOS can be used as a promising adjunct therapy for 
      treatment of HHcy-induced VaD and oxidative stress.
FAU - Pasangulapati, Jagadeesh Prasad
AU  - Pasangulapati JP
AD  - Department of Pharmacology, Treventis Corporation, Toronto, Ontario, Canada.
FAU - Ravula, Arun Reddy
AU  - Ravula AR
AD  - Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ, USA.
FAU - Kanala, Dinesh Reddy
AU  - Kanala DR
AD  - Pharmaceutical Sciences, Mohawk-McMaster Institute for Applied Health Sciences, 
      West Hamilton, Ontario, Canada.
FAU - Boyina, Shanmukhi
AU  - Boyina S
AD  - Drug Toxicology Division, National Institute of Nutrition, Indian Council of 
      Medical Research, Hyderabad, Telangana, India.
FAU - Gangarapu, Kiran
AU  - Gangarapu K
AD  - Department of Pharmaceutical Analysis, School of Pharmacy, Anurag Group of 
      Institutions, Hyderabad, Telangana, India.
FAU - Boyina, Hemanth Kumar
AU  - Boyina HK
AD  - Department of Pharmacology, School of Pharmacy, Anurag Group of Institutions, 
      Hyderabad, Telangana, India. hemanthboyina6@outlook.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Plant Extracts)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
EIN - Adv Exp Med Biol. 2020;1195:C1. PMID: 33415578
MH  - Animals
MH  - Brain/drug effects/metabolism
MH  - Dementia, Vascular/blood/*drug therapy/*etiology/metabolism
MH  - Homocysteine/blood/metabolism
MH  - Hyperhomocysteinemia/blood/*complications
MH  - Maze Learning/drug effects
MH  - Molecular Docking Simulation
MH  - Nitric Oxide/metabolism
MH  - Ocimum sanctum/*chemistry
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/chemistry/pharmacology/*therapeutic use
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Acetylcholinesterase
OT  - Endothelial dysfunction
OT  - Homocysteine
OT  - L-methionine
OT  - Molecular docking
OT  - Molludistin
OT  - Morris water maze
EDAT- 2020/05/30 06:00
MHDA- 2020/09/12 06:00
CRDT- 2020/05/30 06:00
PHST- 2020/05/30 06:00 [entrez]
PHST- 2020/05/30 06:00 [pubmed]
PHST- 2020/09/12 06:00 [medline]
AID - 10.1007/978-3-030-32633-3_30 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 2020;1195:213-225. doi: 10.1007/978-3-030-32633-3_30.