PMID- 32470472
OWN - NLM
STAT- MEDLINE
DCOM- 20201218
LR  - 20201218
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 295
DP  - 2020 Sep 1
TI  - Ectopic over expression of kiss1 may compensate for the loss of kiss2.
PG  - 113523
LID - S0016-6480(20)30276-8 [pii]
LID - 10.1016/j.ygcen.2020.113523 [doi]
AB  - Kisspeptin (KISS) is a neuropeptide which plays a central role in the regulation 
      of the hypothalamic-pituitary-gonadal axis, and is essential for sexual 
      maturation and fertility in mammals. Unlike mammals, which possess only one KISS 
      gene, two paralogous genes, kiss1 and kiss2, have been identified in zebrafish 
      and other non-mammalian vertebrates. Previous studies suggest that Kiss2, but not 
      Kiss1, is the reproduction relevant form amongst the two. To better understand 
      the role of each of these isoforms in reproduction, a loss of function approach 
      was applied. Two genetic manipulation techniques-clustered regularly interspaced 
      short palindromic repeats (CRISPR) and transcription activator-like effector 
      nucleases (TALEN)-were used to generate kiss1 and kiss2 knockout (KO) zebrafish 
      lines, respectively. Examination of these KO lines showed that reproductive 
      capability was not impaired, confirming earlier observations. Further analysis 
      revealed that KO of kiss2 caused a significant increase in expression levels of 
      kiss1, kiss2r and tac3a, while KO of kiss1 had no effect on the expression of any 
      of the examined genes. In situ hybridization analysis revealed that kiss1 mRNA is 
      expressed only in the habenula in wild type brains, while in kiss2 KO fish, kiss1 
      mRNA-expressing cells were identified also in the ventral telencephalon, the 
      ventral part of the entopeduncular nucleus, and the dorsal and ventral 
      hypothalamus. Interestingly, these regions are known to express kiss2r, and the 
      ventral hypothalamus normally expresses kiss2. These results suggest that a 
      compensatory mechanism, involving ectopic kiss1 expression, takes place in the 
      kiss2 KO fish, which may substitute for Kiss2 activity.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Etzion, Talya
AU  - Etzion T
AD  - Department of Neurobiology, George S. Wise Faculty of Life Sciences and Sagol 
      School of Neurosciences, University of Tel Aviv, Tel Aviv 69978, Israel.
FAU - Zmora, Nilli
AU  - Zmora N
AD  - Department of Marine Biotechnology, Institute of Marine and Environmental 
      Technology, University of Maryland Baltimore County, Baltimore, MD, USA.
FAU - Zohar, Yonatan
AU  - Zohar Y
AD  - Department of Marine Biotechnology, Institute of Marine and Environmental 
      Technology, University of Maryland Baltimore County, Baltimore, MD, USA.
FAU - Levavi-Sivan, Berta
AU  - Levavi-Sivan B
AD  - Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, 
      and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel.
FAU - Golan, Matan
AU  - Golan M
AD  - Department of Poultry and Aquaculture, Institute of Animal Sciences, Agricultural 
      Research Organization, Volcani Center, Rishon Letziyon 7505101, Israel.
FAU - Gothilf, Yoav
AU  - Gothilf Y
AD  - Department of Neurobiology, George S. Wise Faculty of Life Sciences and Sagol 
      School of Neurosciences, University of Tel Aviv, Tel Aviv 69978, Israel. 
      Electronic address: yoavgothilf@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200527
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Gonadotropins)
RN  - 0 (Kisspeptins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Zebrafish Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Knockout Techniques
MH  - Gonadotropins/genetics/metabolism
MH  - Kisspeptins/*metabolism
MH  - Male
MH  - Mutation/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Reproduction/genetics
MH  - Zebrafish/*metabolism
MH  - Zebrafish Proteins/genetics/*metabolism
OTO - NOTNLM
OT  - Compensatory mechanism
OT  - Kisspeptin
OT  - Knockout
OT  - Reproduction
OT  - Tachykinin
OT  - Zebrafish
EDAT- 2020/05/30 06:00
MHDA- 2020/12/19 06:00
CRDT- 2020/05/30 06:00
PHST- 2020/03/08 00:00 [received]
PHST- 2020/05/19 00:00 [revised]
PHST- 2020/05/22 00:00 [accepted]
PHST- 2020/05/30 06:00 [pubmed]
PHST- 2020/12/19 06:00 [medline]
PHST- 2020/05/30 06:00 [entrez]
AID - S0016-6480(20)30276-8 [pii]
AID - 10.1016/j.ygcen.2020.113523 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2020 Sep 1;295:113523. doi: 10.1016/j.ygcen.2020.113523. 
      Epub 2020 May 27.