PMID- 32471989
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20210529
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 May 29
TI  - Spatio-temporal patterning of different connexins in developing and postnatal 
      human kidneys and in nephrotic syndrome of the Finnish type (CNF).
PG  - 8756
LID - 10.1038/s41598-020-65777-5 [doi]
LID - 8756
AB  - Connexins (Cxs) are membrane-spanning proteins which enable flow of information 
      important for kidney homeostasis. Changes in their spatiotemporal patterning 
      characterize blood vessel abnormalities and chronic kidney diseases (CKD). We 
      analysed spatiotemporal expression of Cx37, Cx40, Cx43 and Cx45 in nephron and 
      glomerular cells of developing, postnatal kidneys, and nephrotic syndrome of the 
      Finnish type (CNF) by using immunohistochemistry, statistical methods and 
      electron microscopy. During kidney development, strong Cx45 expression in 
      proximal tubules and decreasing expression in glomeruli was observed. In 
      developing distal nephron, Cx37 and Cx40 showed moderate-to-strong expression, 
      while weak Cx43 expression gradually increased. Cx45/Cx40 co-localized in 
      mesangial and granular cells. Cx43 /Cx45 co-localized in podocytes, mesangial and 
      parietal epithelial cells, and with podocyte markers (synaptopodin, nephrin). 
      Different Cxs co-expressed with endothelial (CD31) and VSMC (alpha -SMA) markers in 
      vascular walls. Peak signalling of Cx37, Cx43 and Cx40 accompanied kidney 
      nephrogenesis, while strongest Cx45 signalling paralleled nephron maturation. 
      Spatiotemporal Cxs patterning indicate participation of Cx45 in differentiation 
      of proximal tubules, and Cx43, Cx37 and Cx40 in distal tubules differentiation. 
      CNF characterized disorganized Cx45 expression in proximal tubules, increased 
      Cx43 expression in distal tubules and overall elevation of Cx40 and Cx37, while 
      Cx40 co-localized with increased number of interstitial myofibroblasts.
FAU - Kosovic, Ivona
AU  - Kosovic I
AD  - Department of Anatomy, Histology and Embryology, School of Medicine, University 
      of Split, Split, Croatia.
FAU - Filipovic, Natalija
AU  - Filipovic N
AD  - Department of Anatomy, Histology and Embryology, School of Medicine, University 
      of Split, Split, Croatia.
FAU - Benzon, Benjamin
AU  - Benzon B
AD  - Department of Anatomy, Histology and Embryology, School of Medicine, University 
      of Split, Split, Croatia.
FAU - Vukojevic, Katarina
AU  - Vukojevic K
AD  - Department of Anatomy, Histology and Embryology, School of Medicine, University 
      of Split, Split, Croatia.
AD  - Department of Histology and Embryology, School of Medicine, University of Mostar, 
      Mostar, Bosnia and Herzegovina.
FAU - Saraga, Marijan
AU  - Saraga M
AD  - Department of Paediatrics, University Hospital in Split, School of Medicine, 
      University of Split, Split, Croatia.
FAU - Glavina Durdov, Merica
AU  - Glavina Durdov M
AD  - Department of Pathology, University Hospital in Split, School of Medicine, 
      University of Split, Split, Croatia.
FAU - Bocina, Ivana
AU  - Bocina I
AD  - Department of Biology, Faculty of Science, University of Split, Split, Croatia.
FAU - Saraga-Babic, Mirna
AU  - Saraga-Babic M
AUID- ORCID: 0000-0002-9776-2532
AD  - Department of Anatomy, Histology and Embryology, School of Medicine, University 
      of Split, Split, Croatia. msb@mefst.hr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200529
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (ACTA2 protein, human)
RN  - 0 (Actins)
RN  - 0 (Connexins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (PECAM1 protein, human)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (SYNPO protein, human)
RN  - 0 (nephrin)
SB  - IM
MH  - Actins/biosynthesis/genetics
MH  - Connexins/*biosynthesis/genetics
MH  - Gap Junctions/ultrastructure
MH  - *Gene Expression Regulation, Developmental
MH  - Gestational Age
MH  - Humans
MH  - Infant
MH  - Kidney/embryology/growth & development/*metabolism/ultrastructure
MH  - Male
MH  - Membrane Proteins/deficiency
MH  - Mesenchymal Stem Cells/metabolism
MH  - Microfilament Proteins/biosynthesis/genetics
MH  - Mutation, Missense
MH  - Nephrotic Syndrome/genetics/*metabolism
MH  - Organ Specificity
MH  - Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis/genetics
PMC - PMC7260365
COIS- The authors declare no competing interests.
EDAT- 2020/05/31 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/05/29
CRDT- 2020/05/31 06:00
PHST- 2020/02/19 00:00 [received]
PHST- 2020/05/06 00:00 [accepted]
PHST- 2020/05/31 06:00 [entrez]
PHST- 2020/05/31 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/05/29 00:00 [pmc-release]
AID - 10.1038/s41598-020-65777-5 [pii]
AID - 65777 [pii]
AID - 10.1038/s41598-020-65777-5 [doi]
PST - epublish
SO  - Sci Rep. 2020 May 29;10(1):8756. doi: 10.1038/s41598-020-65777-5.