PMID- 32472399
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 1896-1851 (Electronic)
IS  - 1230-2821 (Linking)
VI  - 65
IP  - 4
DP  - 2020 Dec
TI  - Enhancing the Stability of Haemonchus contortus Glyceraldehyde-3-Phosphate 
      Dehydrogenase and Binding of Host Albumin to the Parasite Enzyme.
PG  - 980-984
LID - 10.2478/s11686-020-00212-3 [doi]
AB  - INTRODUCTION: Haemonchus contortus is an economically important parasite of 
      domestic animals. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) excreted in 
      the ES product of H. contortus can be a promising vaccine candidate for 
      controlling the parasite infection. Unfortunately, the parasite enzyme breaks 
      down rapidly. The current study focusses on stabilizing the recombinant GAPDH 
      (rGAPDH) of H. contortus. METHODS: The rGAPDH was purified and stored in two 
      different buffers (sodium phosphate + EDTA and bicarbonate-sodium chloride) to 
      check the stability. The affinity of the parasite enzyme towards host serum 
      (Goat) components was evaluated by affinity chromatography. The interacting 
      component was identified by mass spectrometry. RESULTS: Here, we report that the 
      enzyme can be stabilized for at least 3 months if stored in bicarbonate-sodium 
      chloride. This should facilitate testing of the enzyme in challenge trials. 
      Additionally, we show that the parasite enzyme has affinity for host albumin; 
      this interaction may have significance in host-parasite relationship. CONCLUSION: 
      The present study reports a combination of sodium bicarbonate (0.1 M) with 0.5 M 
      sodium chloride as a suitable buffer to enhance the stability of H. contortus 
      GAPDH.
FAU - Nijo, Yapu
AU  - Nijo Y
AD  - Division of Biochemistry, ICAR-IVRI, Izatnagar, 243122, India.
FAU - Mishra, Prasanta Kumar K
AU  - Mishra PKK
AD  - Unit of Veterinary Clinical Complex, FVAS, RGSC, Banaras Hindu University, 
      Varanasi, 231001, India. prasantmodel@gmail.com.
FAU - Joshi, Paritosh
AU  - Joshi P
AD  - Division of Biochemistry, ICAR-IVRI, Izatnagar, 243122, India.
LA  - eng
PT  - Journal Article
DEP - 20200529
PL  - Switzerland
TA  - Acta Parasitol
JT  - Acta parasitologica
JID - 9301947
RN  - 0 (Albumins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Albumins
MH  - Animals
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases
MH  - *Goat Diseases
MH  - Goats
MH  - *Haemonchiasis/veterinary
MH  - *Haemonchus
MH  - *Parasites
OTO - NOTNLM
OT  - Albumin
OT  - Buffer
OT  - GAPDH
OT  - Haemonchus contortus
OT  - Stability
EDAT- 2020/05/31 06:00
MHDA- 2021/08/19 06:00
CRDT- 2020/05/31 06:00
PHST- 2019/12/21 00:00 [received]
PHST- 2020/04/07 00:00 [accepted]
PHST- 2020/05/31 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
PHST- 2020/05/31 06:00 [entrez]
AID - 10.2478/s11686-020-00212-3 [pii]
AID - 10.2478/s11686-020-00212-3 [doi]
PST - ppublish
SO  - Acta Parasitol. 2020 Dec;65(4):980-984. doi: 10.2478/s11686-020-00212-3. Epub 
      2020 May 29.