PMID- 32472435
OWN - NLM
STAT- MEDLINE
DCOM- 20210713
LR  - 20210713
IS  - 1568-5608 (Electronic)
IS  - 0925-4692 (Print)
IS  - 0925-4692 (Linking)
VI  - 28
IP  - 6
DP  - 2020 Dec
TI  - Paeoniflorin ameliorates ulcerative colitis by modulating the dendritic 
      cell-mediated T(H)17/T(reg) balance.
PG  - 1705-1716
LID - 10.1007/s10787-020-00722-6 [doi]
AB  - Immunological tolerance is critical for maintaining gut homeostasis. An imbalance 
      between interleukin-17 (IL-17)-producing T helper 17 (T(H)17) cells and 
      regulatory T cells (T(reg) cells) is involved in ulcerative colitis (UC) 
      pathogenesis. Dendritic cells (DCs) are able to induce T cell differentiation. 
      Paeoniflorin (PF) is a monoterpene glucoside that is commonly used for treatment 
      of autoimmune disease. However, the immunological mechanism of PF involvement in 
      UC treatment is unclear. The present study aimed to explore whether PF can 
      restore the T(H)17/T(reg) balance by modulating DCs. The effects of PF on DCs, 
      T(H)17 cells and T(reg) cells were measured. Furthermore, PF-treated DCs were 
      injected into mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced 
      colitis. PF inhibited MHC-II and CD86 expression on the DC surface (P < 0.05), 
      decreased interleukin (IL)-12 secretion in vitro and in vivo (P < 0.05), and 
      restored the T(H)17/T(reg) ratio in the mouse model of colitis (P < 0.05). 
      PF-treated DCs diminished T(H)17 differentiation (4.26% in vitro and 1.64% in 
      vivo) and decreased IL-17 expression (P < 0.05) while inducing 
      CD4(+)CD25(+)Foxp3(+) T(reg) differentiation (7.82% in vitro and 6.85% in vivo) 
      and increasing Foxp3 and IL-10 production (P < 0.05). Additionally, both PF and 
      PF-treated DCs improved colonic histopathology in the mouse model of colitis 
      (P < 0.05). In conclusion this study suggested that PF can ameliorate 
      TNBS-induced colitis by modulating the DC-mediated T(H)17/T(reg) balance.
FAU - Zheng, Kai
AU  - Zheng K
AUID- ORCID: 0000-0001-7005-0271
AD  - Department of Gastroenterology, Affiliated Hospital of Nanjing University of 
      Chinese Medicine, Nanjing, Jiangsu, China. pajo@163.com.
FAU - Jia, Jia
AU  - Jia J
AD  - Department of Endocrinology, Affiliated Hospital of Nanjing University of Chinese 
      Medicine, Nanjing, Jiangsu, China.
FAU - Yan, Shihai
AU  - Yan S
AD  - Laboratory of Pharmacology, Affiliated Hospital of Nanjing University of Chinese 
      Medicine, Nanjing, Jiangsu, China.
FAU - Shen, Hong
AU  - Shen H
AD  - Department of Gastroenterology, Affiliated Hospital of Nanjing University of 
      Chinese Medicine, Nanjing, Jiangsu, China.
FAU - Zhu, Ping
AU  - Zhu P
AD  - Department of Colon and Rectal Surgery, Affiliated Hospital of Nanjing University 
      of Chinese Medicine, Nanjing, Jiangsu, China.
FAU - Yu, Jiangyi
AU  - Yu J
AD  - Department of Endocrinology, Affiliated Hospital of Nanjing University of Chinese 
      Medicine, Nanjing, Jiangsu, China. tcmstudy@163.com.
LA  - eng
GR  - 81873250/National Natural Science Foundation of China/
GR  - 81704022/National Natural Science Foundation of China/
GR  - 81403343/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200529
PL  - Switzerland
TA  - Inflammopharmacology
JT  - Inflammopharmacology
JID - 9112626
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Glucosides)
RN  - 0 (Interleukin-17)
RN  - 0 (Monoterpenes)
RN  - 130068-27-8 (Interleukin-10)
RN  - 21AIQ4EV64 (peoniflorin)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - Autoimmune Diseases/drug therapy/metabolism
MH  - Cell Differentiation/drug effects
MH  - Colitis, Ulcerative/*drug therapy/metabolism
MH  - Dendritic Cells/*drug effects/metabolism
MH  - Disease Models, Animal
MH  - Forkhead Transcription Factors/metabolism
MH  - Glucosides/metabolism/*pharmacology
MH  - Interleukin-10/metabolism
MH  - Interleukin-17/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monoterpenes/*pharmacology
MH  - T-Lymphocytes, Regulatory/*drug effects/metabolism
MH  - Th17 Cells/*drug effects/metabolism
MH  - Trinitrobenzenesulfonic Acid/pharmacology
PMC - PMC7572351
OTO - NOTNLM
OT  - Dendritic cells
OT  - Immunological tolerance
OT  - Paeoniflorin
OT  - TH17
OT  - Treg
OT  - Ulcerative colitis
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/05/31 06:00
MHDA- 2021/07/14 06:00
PMCR- 2020/05/29
CRDT- 2020/05/31 06:00
PHST- 2019/11/17 00:00 [received]
PHST- 2020/05/11 00:00 [accepted]
PHST- 2020/05/31 06:00 [pubmed]
PHST- 2021/07/14 06:00 [medline]
PHST- 2020/05/31 06:00 [entrez]
PHST- 2020/05/29 00:00 [pmc-release]
AID - 10.1007/s10787-020-00722-6 [pii]
AID - 722 [pii]
AID - 10.1007/s10787-020-00722-6 [doi]
PST - ppublish
SO  - Inflammopharmacology. 2020 Dec;28(6):1705-1716. doi: 10.1007/s10787-020-00722-6. 
      Epub 2020 May 29.