PMID- 32473752
OWN - NLM
STAT- MEDLINE
DCOM- 20210113
LR  - 20210113
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 528
IP  - 2
DP  - 2020 Jul 23
TI  - The identification of articular cartilage and growth plate extracellular 
      matrix-specific proteins supportive of either osteogenesis or stable 
      chondrogenesis of stem cells.
PG  - 285-291
LID - S0006-291X(20)30981-5 [pii]
LID - 10.1016/j.bbrc.2020.05.074 [doi]
AB  - Tissue-specific extracellular matrix (ECM) proteins can play a key role in 
      regulating the fate of stem cells and can potentially be utilized for therapeutic 
      applications. Realising this potential requires further characterization of the 
      diversity of biomolecules present in tissue-specific ECMs and an evaluation of 
      their role as regulatory cues for regenerative medicine applications. The goal of 
      this study was to identify specific soluble factors within the ECM of articular 
      cartilage (AC) and growth plate (GP) that may impart chondro-inductivity or 
      osteo-inductivity respectively. To this end, the significantly different proteins 
      between both matrisomes were searched against the STRING database platform, from 
      which C-type lectin domain family-11 member-A (CLEC11A) and S100 calcium-binding 
      protein-A10 (S100A10) were identified as potential candidates for supporting 
      osteogenesis, and Gremlin-1 (GREM1) and TGF-beta induced gene human clone-3 (betaIGH3) 
      were identified as potential candidates for supporting stable chondrogenesis. 
      Stimulation of chondrogenically-primed bone marrow-derived stem cells (BMSCs) 
      with the AC-specific proteins GREM1 and betaIGH3 had no noticeable effect on the 
      deposition of collagen-II, a marker of chondrogenesis, but appeared to suppress 
      the production of the hypertrophic marker collagen-X, particularly for higher 
      concentrations of GREM1. Stimulation with GREM1 was also found to suppress the 
      direct osteoblastic differentiation of BMSCs. In contrast, stimulation with the 
      GP-specific factors CLEC11A and S100A10 was found to enhance osteogenesis of 
      BMSCs, increasing the levels of mineralization, particularly for higher 
      concentration of CLEC11A. Together these results demonstrate that AC- and 
      GP-specific proteins may play a key role in developing novel strategies for 
      engineering phenotypically stable articular cartilage or enhancing the 
      regeneration of critically-sized bone defects.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Diaz-Payno, Pedro J
AU  - Diaz-Payno PJ
AD  - Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, 
      Trinity College Dublin, Dublin, Ireland; Department of Mechanical and 
      Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, 
      Ireland.
FAU - Browe, David C
AU  - Browe DC
AD  - Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, 
      Trinity College Dublin, Dublin, Ireland; Department of Mechanical and 
      Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, 
      Ireland; Advanced Materials and Bioengineering Research Centre (AMBER), Royal 
      College of Surgeons in Ireland and Trinity College Dublin, Dublin, Ireland.
FAU - Cunniffe, Grainne M
AU  - Cunniffe GM
AD  - Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, 
      Trinity College Dublin, Dublin, Ireland; Department of Mechanical and 
      Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, 
      Ireland; Advanced Materials and Bioengineering Research Centre (AMBER), Royal 
      College of Surgeons in Ireland and Trinity College Dublin, Dublin, Ireland.
FAU - Kelly, Daniel J
AU  - Kelly DJ
AD  - Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, 
      Trinity College Dublin, Dublin, Ireland; Department of Mechanical and 
      Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin, 
      Ireland; Advanced Materials and Bioengineering Research Centre (AMBER), Royal 
      College of Surgeons in Ireland and Trinity College Dublin, Dublin, Ireland; 
      Tissue Engineering Research Group, Department of Anatomy, Royal College of 
      Surgeons in Ireland, Dublin, Ireland. Electronic address: kellyd9@tcd.ie.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200527
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Extracellular Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*metabolism
MH  - *Chondrogenesis
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Growth Plate/*metabolism
MH  - Hypertrophy
MH  - Mesenchymal Stem Cells/*metabolism/pathology
MH  - *Osteogenesis
MH  - Swine
OTO - NOTNLM
OT  - Cartilage
OT  - Extracellular matrix
OT  - Growth plate
OT  - Matrisome
COIS- Declaration of competing interest The authors declare that they have no competing 
      interests.
EDAT- 2020/06/01 06:00
MHDA- 2021/01/14 06:00
CRDT- 2020/06/01 06:00
PHST- 2020/04/28 00:00 [received]
PHST- 2020/05/11 00:00 [accepted]
PHST- 2020/06/01 06:00 [pubmed]
PHST- 2021/01/14 06:00 [medline]
PHST- 2020/06/01 06:00 [entrez]
AID - S0006-291X(20)30981-5 [pii]
AID - 10.1016/j.bbrc.2020.05.074 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2020 Jul 23;528(2):285-291. doi: 
      10.1016/j.bbrc.2020.05.074. Epub 2020 May 27.