PMID- 32474688
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20200720
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 165
IP  - 8
DP  - 2020 Aug
TI  - Generation of a porcine reproductive and respiratory syndrome virus expressing a 
      marker gene inserted between ORF4 and ORF5a.
PG  - 1803-1813
LID - 10.1007/s00705-020-04679-3 [doi]
AB  - In recent years, the availability of reverse genetics systems for porcine 
      reproductive and respiratory syndrome virus (PRRSV) has created new perspectives 
      for the use of recombinant viruses as expression vectors. Most of these 
      recombinant PRRSV vectors express foreign genes through either an independent 
      transcription unit inserted in ORF1b and ORF2, or in ORF7 and the 3' UTR. The aim 
      of this study was to find an alternative site for foreign gene insertion into the 
      PRRSV genome. Here, we constructed an infectious cDNA clone for a cell-adapted 
      PRRSV strain, GXNN1396-P96. This cDNA-clone-derived recombinant virus (rGXAM) was 
      comparable in its growth kinetics in MARC-145 cells to the parental virus, 
      GX1396-P96. Using the infectious cDNA-clone, we inserted an independent 
      transcription unit in ORF4 and ORF5a to generate a novel PRRSV-based recombinant 
      virus expressing the green fluorescent protein (GFP) gene. Biological 
      characterization of the recombinant virus, rGX45BSTRS-GFP, showed that it 
      maintained similar growth characteristics but produced fewer infectious virions 
      than the parental PRRSV. These data demonstrate that the ORF4 and ORF5a site is 
      able to tolerate the insertion of foreign genes.
FAU - Wang, Yuxu
AU  - Wang Y
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - He, Wei
AU  - He W
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Li, Qingqing
AU  - Li Q
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Xie, Xin
AU  - Xie X
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Qin, Nian
AU  - Qin N
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Wang, Hao
AU  - Wang H
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Huang, Jiabin
AU  - Huang J
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Lin, Siyuan
AU  - Lin S
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Ouyang, Kang
AU  - Ouyang K
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Chen, Ying
AU  - Chen Y
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Huang, Weijian
AU  - Huang W
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China.
FAU - Wei, Zuzhang
AU  - Wei Z
AUID- ORCID: 0000-0002-6317-2315
AD  - Laboratory of Animal infectious Diseases and molecular Immunology, College of 
      Animal Science and Technology, Guangxi University, No. 100 Daxue Road, Nanning, 
      530004, China. zuzhangwei@gxu.edu.cn.
LA  - eng
GR  - 31660716/National Natural Science Foundation of China/
GR  - 31372444/National Natural Science Foundation of China/
GR  - 2018GXNSFDA281021/Natural Science Foundation of Guangxi Province/
PT  - Journal Article
DEP - 20200530
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Genetic Markers)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Genetic Markers/*genetics
MH  - Green Fluorescent Proteins/genetics
MH  - Open Reading Frames/*genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Swine
MH  - Virus Replication/genetics
EDAT- 2020/06/01 06:00
MHDA- 2020/07/21 06:00
CRDT- 2020/06/01 06:00
PHST- 2020/01/02 00:00 [received]
PHST- 2020/04/24 00:00 [accepted]
PHST- 2020/06/01 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
PHST- 2020/06/01 06:00 [entrez]
AID - 10.1007/s00705-020-04679-3 [pii]
AID - 10.1007/s00705-020-04679-3 [doi]
PST - ppublish
SO  - Arch Virol. 2020 Aug;165(8):1803-1813. doi: 10.1007/s00705-020-04679-3. Epub 2020 
      May 30.