PMID- 32474879
OWN - NLM
STAT- MEDLINE
DCOM- 20210317
LR  - 20210317
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2155
DP  - 2020
TI  - Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts.
PG  - 201-212
LID - 10.1007/978-1-0716-0655-1_17 [doi]
AB  - In the tissue culture dish, osteoblast cells can be derived from mesenchymal stem 
      cells (MSCs) and pluripotent stem cells (PSCs) including embryonic stem cells 
      (ESCs) and induced pluripotent stem cells (iPSCs). However, differentiation of 
      osteoblasts from PSCs is time-consuming and low yield. In contrast, we identified 
      four osteogenic transcription factors, Runx2, Osx, Dlx5, and ATF4, that rapidly 
      and efficiently reprogram mouse fibroblasts derived from 2.3 kb type I collagen 
      promoter-driven green fluorescent protein (Col2.3GFP) transgenic mice into 
      induced osteoblast cells (iOBs). iOBs exhibit osteoblast morphology, form 
      mineralized nodules, and express Col2.3GFP and gene markers of osteoblast 
      differentiation. Our method provides a robust system to rapidly generate 
      appropriate and abundant osteoblast cells for osteogenesis and bone regeneration 
      study.
FAU - Zhu, Hui
AU  - Zhu H
AD  - Division of Endocrinology, Stanford University School of Medicine, Stanford, CA, 
      USA.
FAU - Wu, Joy Y
AU  - Wu JY
AD  - Division of Endocrinology, Stanford University School of Medicine, Stanford, CA, 
      USA. jywu1@stanford.edu.
LA  - eng
GR  - DP2 OD008466/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Bone Regeneration
MH  - Calcification, Physiologic
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cell Separation/methods
MH  - *Cellular Reprogramming/genetics
MH  - *Cellular Reprogramming Techniques
MH  - Fibroblasts/*cytology/*metabolism
MH  - Genetic Vectors/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Osteoblasts/*cytology/*metabolism
MH  - Osteogenesis
MH  - Transduction, Genetic
MH  - Transgenes
OTO - NOTNLM
OT  - Bone
OT  - Fibroblast
OT  - Osteoblast
OT  - Reprogramming
EDAT- 2020/06/01 06:00
MHDA- 2021/03/18 06:00
CRDT- 2020/06/01 06:00
PHST- 2020/06/01 06:00 [entrez]
PHST- 2020/06/01 06:00 [pubmed]
PHST- 2021/03/18 06:00 [medline]
AID - 10.1007/978-1-0716-0655-1_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2155:201-212. doi: 10.1007/978-1-0716-0655-1_17.