PMID- 32474960
OWN - NLM
STAT- MEDLINE
DCOM- 20210402
LR  - 20210402
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 235
IP  - 12
DP  - 2020 Dec
TI  - miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating 
      circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
PG  - 10051-10067
LID - 10.1002/jcp.29821 [doi]
AB  - microRNAs (miRNAs) and circular RNAs (circRNAs) are important for endometrial 
      receptivity establishment and embryo implantation in mammals. miR-34a and miR-34c 
      are highly expressed in caprine receptive endometrium (RE). Herein, the functions 
      and mechanisms of miR-34a/c in caprine endometrial epithelial cell (CEEC) 
      apoptosis and RE establishment were investigated. miR-34a/c downregulated the 
      expression level of centrosomal protein 55 (CEP55) and were sponged by 
      circRNA8073 (circ-8073), thereby exhibiting a negative interaction in CEEC. 
      miR-34a/c induced CEEC apoptosis by targeting circ-8073/CEP55 through the 
      regulation of the RAS/RAF/MEK/ERK and phosphoitide 3-kinase (PI3K)/protein kinase 
      B (AKT)/mammalian target of rapamycin (mTOR) pathways. Positive and negative 
      feedback loops and cross-talk were documented between the RAS/RAF/MEK/ERK and 
      PI3K/AKT/mTOR pathways. miR-34a/c regulated the levels of RE marker genes, 
      including forkhead box M1, vascular endothelial growth factor, and osteopontin 
      (OPN). These results suggest that miR-34a/c not only induce CEEC apoptosis by 
      binding to circ-8073 and CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR 
      pathways, but may also regulate RE establishment in dairy goats.
CI  - (c) 2020 Wiley Periodicals LLC.
FAU - Liu, Xiaorui
AU  - Liu X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Yang, Lichun
AU  - Yang L
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Cui, Jiuzeng
AU  - Cui J
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Che, Sicheng
AU  - Che S
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Liu, Yuexia
AU  - Liu Y
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Han, Jincheng
AU  - Han J
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - An, Xiaopeng
AU  - An X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Cao, Binyun
AU  - Cao B
AUID- ORCID: 0000-0002-9649-3246
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
FAU - Song, Yuxuan
AU  - Song Y
AUID- ORCID: 0000-0002-5302-7835
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200531
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cep55 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (mTOR Associated Protein, LST8 Homolog)
RN  - EC 2.7.11.1 (raf Kinases)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Cycle Proteins/genetics
MH  - Embryo Implantation/*genetics
MH  - Endometrium/growth & development/metabolism
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Goats/genetics/growth & development
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - RNA, Circular/*genetics
MH  - Signal Transduction/genetics
MH  - mTOR Associated Protein, LST8 Homolog
MH  - raf Kinases/genetics
MH  - ras Proteins/genetics
OTO - NOTNLM
OT  - CEP55
OT  - apoptosis
OT  - caprine endometrial epithelial cell
OT  - circ-8073
OT  - miR-34a/c
OT  - receptive endometrium
EDAT- 2020/06/01 06:00
MHDA- 2021/04/07 06:00
CRDT- 2020/06/01 06:00
PHST- 2019/09/25 00:00 [received]
PHST- 2020/05/16 00:00 [revised]
PHST- 2020/05/16 00:00 [accepted]
PHST- 2020/06/01 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
PHST- 2020/06/01 06:00 [entrez]
AID - 10.1002/jcp.29821 [doi]
PST - ppublish
SO  - J Cell Physiol. 2020 Dec;235(12):10051-10067. doi: 10.1002/jcp.29821. Epub 2020 
      May 31.