PMID- 32475388
OWN - NLM
STAT- MEDLINE
DCOM- 20210111
LR  - 20210111
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1120
DP  - 2020 Jul 11
TI  - Quantitative profiling of eicosanoids derived from n-6 and n-3 polyunsaturated 
      fatty acids by twin derivatization strategy combined with LC-MS/MS in patients 
      with type 2 diabetes mellitus.
PG  - 24-35
LID - S0003-2670(20)30491-8 [pii]
LID - 10.1016/j.aca.2020.04.064 [doi]
AB  - Eicosanoids derived from n-6 and n-3 polyunsaturated fatty acids (PUFAs), serving 
      as important signaling molecules, are implicated in many physiological and 
      pathological processes, including Type 2 diabetes mellitus (T2DM). However, the 
      quantification of endogenous eicosanoids is challenged by high structural 
      similarity, low abundance in biological sample and poor electrospray ionization 
      efficiency. In the current study, a sensitive and accurate liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to 
      quantify 65 eicosanoids derived from n-6 and n-3 PUFAs in plasma samples using 
      twin derivatization strategy with a pair of reagents, 5-(dimethylamino) 
      naphthalene-1-sulfonyl piperazine (Dns-PP) and (diethylamino) 
      naphthalene-1-sulfonyl piperazine (Dens-PP). Dns-PP-derivatized plasma sample was 
      mixed with the equal volume of Dens-PP-derivatized eicosanoid internal standards 
      for LC-MS/MS analysis in multiple reaction monitoring (MRM) mode. After Dns-PP 
      derivatization, the ionization efficiency and separation performance were 
      substantially improved, resulting in the enhanced sensitivity by 446- to 
      1009-folds compared to intact eicosanoids. The quantitative accuracy determined 
      by twin derivatization method was found to be comparable with stable isotope 
      labeled internal standards (SIL-IS) method. The newly proposed method was 
      successfully employed to quantify the target eicosanoids in plasma samples from 
      healthy controls and the patients with T2DM. N-6 PUFA-derived eicosanoids, PGF2alpha, 
      PGD2, PGE2, PGA2, PGB2, 20-HETE and LTC4, significantly increased in plasma 
      sample of T2DM patients. Oppositely, n-3 PUFA-derived eicosanoids, RvE1, 
      12(S)-HEPE and RvD1, remarkably decreased. Spearman's correlation analysis 
      indicated the strong correlations between these highlighted eicosanoids and 
      clinical parameters of T2DM. Collectively, the sensitive and reliable eicosanoid 
      quantification method may facilitate to elucidate the characteristics of 
      eicosanoid metabolism and understand the role of eicosanoids in the pathogenesis 
      of T2DM and other diseases.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Xia, Fangbo
AU  - Xia F
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China.
FAU - He, Chengwei
AU  - He C
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China.
FAU - Ren, Meng
AU  - Ren M
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Yanjiang West Road, Guangzhou, Guangdong, China. Electronic address: 
      renmeng80@139.com.
FAU - Xu, Feng-Guo
AU  - Xu FG
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing, Jiangsu, China. Electronic 
      address: fengguoxu@gmail.com.
FAU - Wan, Jian-Bo
AU  - Wan JB
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China. 
      Electronic address: jbwan@um.edu.mo.
LA  - eng
PT  - Journal Article
DEP - 20200428
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Eicosanoids)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Omega-6)
SB  - IM
MH  - Chromatography, Liquid
MH  - Diabetes Mellitus, Type 2/*blood/diagnosis
MH  - Eicosanoids/*blood/chemistry
MH  - Fatty Acids, Omega-3/*chemistry
MH  - Fatty Acids, Omega-6/*chemistry
MH  - Humans
MH  - Molecular Structure
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Chemical isotope labeling
OT  - Eicosanoids
OT  - LC-MS/MS
OT  - Quantification
OT  - Twin derivatization
OT  - Type 2 diabetes mellitus
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/06/02 06:00
MHDA- 2021/01/12 06:00
CRDT- 2020/06/02 06:00
PHST- 2020/03/15 00:00 [received]
PHST- 2020/04/07 00:00 [revised]
PHST- 2020/04/27 00:00 [accepted]
PHST- 2020/06/02 06:00 [entrez]
PHST- 2020/06/02 06:00 [pubmed]
PHST- 2021/01/12 06:00 [medline]
AID - S0003-2670(20)30491-8 [pii]
AID - 10.1016/j.aca.2020.04.064 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2020 Jul 11;1120:24-35. doi: 10.1016/j.aca.2020.04.064. Epub 2020 
      Apr 28.