PMID- 32476367
OWN - NLM
STAT- MEDLINE
DCOM- 20200629
LR  - 20200629
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 36
IP  - 1
DP  - 2020 Jan 28
TI  - [Effects of acute and chronic exercise on fat PI3K/AKT/GLUT4 signal pathway in 
      type 2 diabetic rats].
PG  - 12-16
LID - 10.12047/j.cjap.5802.2020.003 [doi]
AB  - OBJECTIVE: To study the effects of acute and chronic exercise on 
      phosphatidylinositol 3-hydroxy kinase(PI3K)/protein kinase B(AKT)/glucose 
      transporter 4(GLUT4)signaling pathway in adipose tissue of rats with type 2 
      diabetes mellitus (T2DM) induced by high-fat diet and low-dose streptozotocin 
      (STZ). METHODS: A total of 52 SD male rats aged 15 months were randomly divided 
      into normal control group (13) and high-fat group (39), and fed normal and high 
      fat diets. After 8 weeks, the body weight of the high-fat group was higher 20% 
      than that of the normal control group. After a small dose of STZ, the blood 
      glucose level was ＞16.7 mmol/l, and the model was successfully established. The 
      diabetic model group was randomly divided into a diabetic control group (DC, 
      n=13), a diabetic chronic exercise group (DCE, n=13), and a diabetic acute 
      exercise group (DAE, n=13). The DCE group underwent an 8-week swimming exercise 
      and the DAE group performed a one-time swimming exercise. Blood lipids, blood 
      glucose and serum insulin levels were measured, and the contents of fat PI3K, AKT 
      and GLUT4 proteins were determined by Western blot method. RESULTS: The levels of 
      body weight, blood lipids, blood glucose and insulin in the diabetic group were 
      significantly higher than those in the normal control group (P＜0.01); high 
      density liptein cholesterol(HDL-C) levels were decreased (P＜0.05), and the 
      expressions of PI3K, AKT and GLUT4 protein in adipose tissue were decreased 
      (P＜0.01). After 8 weeks of swimming training, the levels of body weight, blood 
      lipids, blood glucose and insulin all were decreased significantly (P＜0.01); 
      while the level of HDL-C was increased (P＜0.05), and the expressions of PI3K, AKT 
      and GLUT4 protein were increased (P＜0.01). After acute exercise, the levels of 
      blood lipids, blood glucose and insulin were decreased (P＜0.05); the level of 
      HDL-C was increased (P＜0.05), and the expression levels of fat PI3K, AKT and 
      GLUT4 were increased significantly (P＜0.05). CONCLUSION: 1 in circleHigh fat diet combined 
      with low-dose STZ induced damage to the PI3K/AKT pathway in adipose tissue of 
      T2DM rats reduced insulin sensitivity. 2 in circleAcute and chronic aerobic exercise can 
      improve the disorder of glucose and lipid metabolism through PI3K/AKT pathway, 
      and the chronic exercise is better than acute exercise.
FAU - Yi, Xue-Jie
AU  - Yi XJ
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Sun, Yu-Xia
AU  - Sun YX
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Yao, Ting-Ting
AU  - Yao TT
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Li, Jing
AU  - Li J
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Gao, Chang
AU  - Gao C
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Liu, Lu
AU  - Liu L
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Cao, Shi-Cheng
AU  - Cao SC
AD  - Department of Sports Medicine, School of Public and Basic Sciences, China Medical 
      University, Shenyang 110001, China.
FAU - Chang, Bo
AU  - Chang B
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
FAU - Zhang, Cui-Ping
AU  - Zhang CP
AD  - School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Blood Glucose)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Lipids)
RN  - 0 (Slc2a4 protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Diabetes Mellitus, Experimental/metabolism/*therapy
MH  - Diabetes Mellitus, Type 2/metabolism/*therapy
MH  - Glucose Transporter Type 4/metabolism
MH  - Insulin/blood
MH  - *Insulin Resistance
MH  - Lipids/blood
MH  - Male
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - *Physical Conditioning, Animal
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Signal Transduction
EDAT- 2020/06/02 06:00
MHDA- 2020/07/01 06:00
CRDT- 2020/06/02 06:00
PHST- 2020/06/02 06:00 [entrez]
PHST- 2020/06/02 06:00 [pubmed]
PHST- 2020/07/01 06:00 [medline]
AID - 10.12047/j.cjap.5802.2020.003 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Jan 28;36(1):12-16. doi: 
      10.12047/j.cjap.5802.2020.003.