PMID- 32478182
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220414
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 6
IP  - 5
DP  - 2020 May
TI  - Majie cataplasm provides a shield against asthmatic punch from the neuroimmune 
      system.
PG  - e03896
LID - 10.1016/j.heliyon.2020.e03896 [doi]
LID - e03896
AB  - Asthma poses a threat to human health, and its pathogenesis is closely related to 
      the neuroimmune system. Majie cataplasm can not only regulate the immune system 
      but also the nervous system in asthma patients for its components. We speculate 
      that Majie cataplasm may relieve asthmatic patients with sensitivity to hormone 
      or not by regulating the body's neuroimmune system. METHODS: In this experiment, 
      a mouse model of asthma was well established by ovalbumin. The lung function of 
      animals was examined and pathological changes in the lung tissue were assessed by 
      hematoxylin-eosin staining. Serum immunoglobulin E (IgE), calcitonin gene-related 
      peptide (CGRP) and neurokinin A (NKA) were measured by ELISA. The location of 
      CGRP, CD3 and neutrophil in lung tissue and their expressions were detected by 
      immunofluorescence staining. In addition, contents of CGRP mRNA, Substance P (SP) 
      mRNA, interleukin (IL)-17 mRNA and interleukin(IL)-13 mRNA were detected by 
      quantitative polymerase chain reaction. RESULTS: Compared with the asthma model 
      group, Majie cataplasm and dexamethasone can not only equivalently relieve airway 
      hyperresponsiveness, but also make the content of serum IgE reduced. In addition, 
      they can lower the content of serum CGRP and NKA after OVA stimulation, and this 
      effect was more obvious for Majie cataplasm. Our results also showed that Majie 
      Cataplasm and dexamethasone could inhibit the secretion of CGRP and the 
      infiltration of T lymphocytes together with neutrophils in lung tissue and reduce 
      expressions of CGRP mRNA, SP mRNA, IL-17 mRNA and IL-13 mRNA in lung tissue. 
      CONCLUSION: Majie cataplasm effectively relieves expressions of neuropeptides 
      such as CGRP, reduces the infiltration of immune cells in lung tissue, regulates 
      the body's neuroimmune system, and has a therapeutic potential for both Th2 
      asthma and neutrophilic asthma.
CI  - (c) 2020 The Authors.
FAU - Ji, Wenting
AU  - Ji W
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Yu, Xue
AU  - Yu X
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Gao, Yushan
AU  - Gao Y
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Ren, Beida
AU  - Ren B
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Zhang, Shuang
AU  - Zhang S
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Wang, Qingguo
AU  - Wang Q
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
FAU - Wang, Xueqian
AU  - Wang X
AD  - Beijing University of Chinese Medicine, Beijing 100029, China.
LA  - eng
PT  - Journal Article
DEP - 20200523
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC7251768
OTO - NOTNLM
OT  - Allergology
OT  - Asthma
OT  - Calcitonin gene-related peptide
OT  - Immune response
OT  - Immunology
OT  - Inflammation
OT  - Majie cataplasm
OT  - Molecular neuroscience
OT  - Neuroscience
OT  - Neutrophils
OT  - Respiratory system
OT  - T cells
OT  - The neuroimmune system
EDAT- 2020/06/02 06:00
MHDA- 2020/06/02 06:01
PMCR- 2020/05/23
CRDT- 2020/06/02 06:00
PHST- 2020/02/07 00:00 [received]
PHST- 2020/03/12 00:00 [revised]
PHST- 2020/04/28 00:00 [accepted]
PHST- 2020/06/02 06:00 [entrez]
PHST- 2020/06/02 06:00 [pubmed]
PHST- 2020/06/02 06:01 [medline]
PHST- 2020/05/23 00:00 [pmc-release]
AID - S2405-8440(20)30741-6 [pii]
AID - e03896 [pii]
AID - 10.1016/j.heliyon.2020.e03896 [doi]
PST - epublish
SO  - Heliyon. 2020 May 23;6(5):e03896. doi: 10.1016/j.heliyon.2020.e03896. eCollection 
      2020 May.