PMID- 32478637
OWN - NLM
STAT- MEDLINE
DCOM- 20211124
LR  - 20211124
IS  - 1532-2750 (Electronic)
IS  - 1098-612X (Linking)
VI  - 23
IP  - 2
DP  - 2021 Feb
TI  - Feline plasma adrenocorticotropic hormone: validation of a chemiluminescent assay 
      and concentrations in cats with hypercortisolism, primary hypoadrenocorticism and 
      other diseases.
PG  - 67-73
LID - 10.1177/1098612X20925686 [doi]
AB  - OBJECTIVES: The aims of this study were to validate a commercially available 
      chemiluminescent assay for measurement of feline plasma adrenocorticotropic 
      hormone concentration (ACTH), to determine the normal reference interval (RI) of 
      plasma ACTH in healthy cats, to assess plasma ACTH in cats with naturally 
      occurring hypercortisolism (HC), primary hypoadrenocorticism (PH) and other 
      diseases (OD), and to evaluate the effect of aprotinin on plasma ACTH 
      degradation. METHODS: Forty healthy cats, 10 with HC, 11 with PH and 30 with OD, 
      were included. The chemiluminescent enzyme immunometric assay was evaluated by 
      measurement of intra-assay precision, interassay precision and linearity. The RI 
      for plasma ACTH in healthy cats was established using robust methods. Plasma ACTH 
      of samples collected with and without aprotinin, stored at 4 degrees C and assayed over a 
      6-day period, was measured. RESULTS: The intra-assay coefficients of variance 
      (CVs) ranged from 2.7% to 4.3% and interassay CVs from 3.3% to 10.7%. Dilution 
      studies showed excellent accuracy (R(2) >0.99). The RI for plasma ACTH in healthy 
      cats was 32-370 pg/ml. Plasma ACTH was not significantly different between 
      healthy cats and the OD group. Cats with pituitary-dependent hypercortisolism 
      (PDH) and PH had significantly higher plasma ACTH than the other groups. Plasma 
      ACTH did not show significant differences when samples collected with and without 
      aprotinin were compared. CONCLUSIONS AND RELEVANCE: The Immulite chemiluminescent 
      assay is a valid technique for measuring plasma ACTH in cats and the RI of plasma 
      ACTH is quite wide. Owing to the low overlap between healthy or OD cats and cats 
      with HC or PH, the measurement of plasma ACTH appears to be useful and should be 
      included in the diagnostic work-up when HC or PH are suspected. Furthermore, the 
      measurement of plasma ACTH may be an accurate test for differentiating PDH from 
      adrenal-dependent hypercortisolism.
FAU - Tardo, Antonio M
AU  - Tardo AM
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, Bologna, Italy.
FAU - Reusch, Claudia E
AU  - Reusch CE
AD  - Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of 
      Zurich, Zurich, Switzerland.
FAU - Galac, Sara
AU  - Galac S
AD  - Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht 
      University, Utrecht, The Netherlands.
FAU - Fornetti, Sofia
AU  - Fornetti S
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, Bologna, Italy.
FAU - Tirolo, Alessandro
AU  - Tirolo A
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, Bologna, Italy.
FAU - Golinelli, Stefania
AU  - Golinelli S
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, Bologna, Italy.
FAU - Shehdula, Dardan
AU  - Shehdula D
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, Bologna, Italy.
FAU - Fracassi, Federico
AU  - Fracassi F
AUID- ORCID: 0000-0003-3121-2199
AD  - Department of Veterinary Medical Sciences, University of Bologna, Ozzano 
      dell'Emilia, Bologna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200601
PL  - England
TA  - J Feline Med Surg
JT  - Journal of feline medicine and surgery
JID - 100897329
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
SB  - IM
MH  - Adrenocorticotropic Hormone
MH  - Animals
MH  - *Cat Diseases/diagnosis
MH  - Cats
MH  - *Cushing Syndrome/veterinary
MH  - Luminescent Measurements/veterinary
OTO - NOTNLM
OT  - Addison's disease
OT  - Cushing's syndrome
OT  - Feline plasma ACTH
OT  - adrenocorticotropic hormone
OT  - aprotinin
OT  - chemiluminescent assay
OT  - reference interval
EDAT- 2020/06/02 06:00
MHDA- 2021/11/25 06:00
CRDT- 2020/06/02 06:00
PHST- 2020/06/02 06:00 [pubmed]
PHST- 2021/11/25 06:00 [medline]
PHST- 2020/06/02 06:00 [entrez]
AID - 10.1177/1098612X20925686 [doi]
PST - ppublish
SO  - J Feline Med Surg. 2021 Feb;23(2):67-73. doi: 10.1177/1098612X20925686. Epub 2020 
      Jun 1.