PMID- 32478715
OWN - NLM
STAT- MEDLINE
DCOM- 20200929
LR  - 20200929
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 159
DP  - 2020 May 18
TI  - Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese 
      Hamster Ovary Cell Cultivation.
LID - 10.3791/60577 [doi]
AB  - Optimization of bioprocesses to increase the yield of desired products is of 
      importance in the biopharmaceutical industry. This can be achieved by strain 
      selection and by developing bioprocess parameters. Shake flasks have been used 
      for this purpose. They, however, lack the capability to control the process 
      parameters such as pH and dissolved oxygen (DO). This limitation can be overcome 
      with the help of an automated micro-bioreactor. These bioreactors mimic 
      cultivation at a larger scale. One of the major advantages of this system is the 
      integration of the Design of Experiment (DOE) in the software. This integration 
      enables establishing a design where multiple process parameters can be varied 
      simultaneously. The critical process parameters and optimum bioprocess conditions 
      can be analyzed within the software. The focus of the work presented here is to 
      introduce the user to the steps involved in process design in the software and 
      incorporation of the DOE within the cultivation run.
FAU - Nagraik, Tamanna
AU  - Nagraik T
AD  - Institute for Technical Chemistry, Gottfried-Wilhelm-Leibniz Universitat 
      Hannover.
FAU - Gonzalez Salcedo, Alina
AU  - Gonzalez Salcedo A
AD  - Institute for Technical Chemistry, Gottfried-Wilhelm-Leibniz Universitat 
      Hannover.
FAU - Solle, Dorte
AU  - Solle D
AD  - Institute for Technical Chemistry, Gottfried-Wilhelm-Leibniz Universitat 
      Hannover; solle@iftc.uni-hannover.de.
FAU - Scheper, Thomas
AU  - Scheper T
AD  - Institute for Technical Chemistry, Gottfried-Wilhelm-Leibniz Universitat 
      Hannover.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20200518
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Bioreactors/*standards
MH  - CHO Cells/*metabolism
MH  - Cricetinae
MH  - Cricetulus
EDAT- 2020/06/02 06:00
MHDA- 2020/09/30 06:00
CRDT- 2020/06/02 06:00
PHST- 2020/06/02 06:00 [entrez]
PHST- 2020/06/02 06:00 [pubmed]
PHST- 2020/09/30 06:00 [medline]
AID - 10.3791/60577 [doi]
PST - epublish
SO  - J Vis Exp. 2020 May 18;(159). doi: 10.3791/60577.