PMID- 32479751 OWN - NLM STAT- MEDLINE DCOM- 20210215 LR - 20210215 IS - 1090-2392 (Electronic) IS - 0011-2240 (Linking) VI - 95 DP - 2020 Aug TI - Quality improvements to mackerel (Scomber japonicus) muscle tissue frozen using a rapid freezer with the weak oscillating magnetic fields. PG - 130-137 LID - S0011-2240(19)30208-1 [pii] LID - 10.1016/j.cryobiol.2020.05.005 [doi] AB - Although freezing is the most popular long-term food preservation method, the formation of ice crystals during the freezing process often degrades the quality of the product. Recently, several reports have argued that oscillating magnetic fields (OMFs) may affect ice crystallization. In this paper, we investigated the effects of OMFs on fresh mackerel using the Cell Alive System(R) (CAS(R)) developed as an additional OMF generator for a rapid freezer. Mackerel fillets were frozen with home freezing (HF), air blast freezing without (ABF) or with CAS (ABF-CAS) (ABI Co. Ltd., Chiba, Japan), and stored them for 2 weeks in the frozen storage between -30 degrees C and -35 degrees C. We analyzed the tissue damages of thawed samples histologically. The OMFs has been shown to significantly inhibit tissue damage in mackerel tissue after freezing and thawing (especially, thawing in ice water). And it seems that OMFs suppressed the ice hole counts (p < 0.05), the mean size (p = 0.061), and the increase of interstitial area% (p < 0.05) after freezing/thawing. We also found that it is necessary to avoid re-crystallization during thawing to maintain the quality of the frozen product. The use of OMFs with rapid thawing has the potential to improve cryopreservation in the food industry as well as in the bioscience industry. CI - Copyright (c) 2020 Elsevier Inc. All rights reserved. FAU - Okuda, Kana AU - Okuda K AD - Department of Molecular Cell Biology, Institute for Bioscience, Mukogawa Women's University, Ikebiraki-cho 6-46, 663-8558, Nishinomiya, Japan; Abi Inc., Ohtakanomori-higashi 1-12-1, 270-0138, Nagareyama, Japan. FAU - Kawauchi, Aiko AU - Kawauchi A AD - Department of Molecular Cell Biology, Institute for Bioscience, Mukogawa Women's University, Ikebiraki-cho 6-46, 663-8558, Nishinomiya, Japan; Abi Inc., Ohtakanomori-higashi 1-12-1, 270-0138, Nagareyama, Japan. FAU - Yomogida, Kentaro AU - Yomogida K AD - Department of Molecular Cell Biology, Institute for Bioscience, Mukogawa Women's University, Ikebiraki-cho 6-46, 663-8558, Nishinomiya, Japan. Electronic address: yomo@mukogawa-u.ac.jp. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200530 PL - Netherlands TA - Cryobiology JT - Cryobiology JID - 0006252 SB - IM MH - Animals MH - *Cryopreservation/methods MH - Freezing MH - Magnetic Fields MH - Muscles MH - *Perciformes MH - Quality Improvement OTO - NOTNLM OT - Air blast-freezer OT - Cell Alive System(R) (CAS(R)) OT - Freezing OT - Frozen storage OT - Ice water OT - Mackerel OT - Muscle fiber OT - Thawing OT - Weak oscillating magnetic fields EDAT- 2020/06/02 06:00 MHDA- 2021/02/16 06:00 CRDT- 2020/06/02 06:00 PHST- 2019/07/11 00:00 [received] PHST- 2020/05/08 00:00 [revised] PHST- 2020/05/08 00:00 [accepted] PHST- 2020/06/02 06:00 [pubmed] PHST- 2021/02/16 06:00 [medline] PHST- 2020/06/02 06:00 [entrez] AID - S0011-2240(19)30208-1 [pii] AID - 10.1016/j.cryobiol.2020.05.005 [doi] PST - ppublish SO - Cryobiology. 2020 Aug;95:130-137. doi: 10.1016/j.cryobiol.2020.05.005. Epub 2020 May 30.