PMID- 32480284
OWN - NLM
STAT- MEDLINE
DCOM- 20210201
LR  - 20210201
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 312
DP  - 2020 Jul
TI  - A new LC-MS/MS confirmation method for the determination of 17 drugs of abuse in 
      oral fluid and its application to real samples.
PG  - 110330
LID - S0379-0738(20)30192-4 [pii]
LID - 10.1016/j.forsciint.2020.110330 [doi]
AB  - A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation 
      method for the direct analysis of 17 drugs starting from 200muL of diluted oral 
      fluid (OF), in a single chromatographic run, was developed and validated. 
      Cocaine, benzoylecgonine (BEG), cocaethylene, Delta-9-tetrahydrocannabinol (Delta-9-THC), 
      buprenorphine, 6-acetylmorphine (6AM), morphine, codeine, methadone, 
      2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), amphetamine, 
      methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 
      methylenedioxyamphetamine (MDA), 3,4-Methylenedioxy-N-ethylamphetamine (MDE), 
      ketamine, N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB) were determined 
      in a chromatographic run of 12min only with no sample pre-treatment, after the 
      addition of 15 different internal standards (ISs). The method met all 
      requirements in terms of linearity, accuracy (precision and trueness), recovery, 
      and stability requested by FDA guidelines. Carry-over and interferences were 
      negligible, as well as the matrix effects. LLOQs are below the limits defined by 
      European guidelines and Italian national laws. The original oral fluid 
      collections are stable at least six months at -20 degrees C and one week at +4 degrees C.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Bassotti, Elisa
AU  - Bassotti E
AD  - R&D Department Eureka Lab Division, Chiaravalle, Italy.
FAU - Merone, Giuseppe Maria
AU  - Merone GM
AD  - Department of Neuroscience, Imaging and Clinical Sciences, University of 
      Chieti-Pescara "G. d'Annunzio", 66100 Chieti, Italy.
FAU - D'Urso, Annachiara
AU  - D'Urso A
AD  - Fondazione IRCCS Istituto Neurologico Carlo Besta, Laboratory of Neurological 
      Biochemistry and Neuropharmacology, Via Celoria 11, Milano 20133, Italy.
FAU - Savini, Fabio
AU  - Savini F
AD  - Laboratory of Pharmatoxicology - Hospital "Santo Spirito", Pescara 65124, Italy.
FAU - Locatelli, Marcello
AU  - Locatelli M
AD  - Department of Pharmacy, University of Chieti-Pescara "G. d'Annunzio", Chieti 
      66100, Italy.
FAU - Tartaglia, Angela
AU  - Tartaglia A
AD  - Department of Pharmacy, University of Chieti-Pescara "G. d'Annunzio", Chieti 
      66100, Italy.
FAU - Dossetto, Paolo
AU  - Dossetto P
AD  - Sciex, Milano, Italy.
FAU - D'Ovidio, Cristian
AU  - D'Ovidio C
AD  - Department of Medicine and Aging Sciences, Section of Legal Medicine, University 
      of Chieti-Pescara "G. d'Annunzio", Chieti 66100, Italy.
FAU - de Grazia, Ugo
AU  - de Grazia U
AD  - Fondazione IRCCS Istituto Neurologico Carlo Besta, Laboratory of Neurological 
      Biochemistry and Neuropharmacology, Via Celoria 11, Milano 20133, Italy. 
      Electronic address: ugo.degrazia@istituto-besta.it.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20200512
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Illicit Drugs)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Forensic Toxicology/methods
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - Saliva/*chemistry
MH  - Specimen Handling
MH  - Substance Abuse Detection/methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Temperature
OTO - NOTNLM
OT  - Confirmation method
OT  - Drugs of abuse
OT  - Forensic toxicology
OT  - Hyphenated configuration
OT  - Oral fluid
COIS- Declaration of Competing Interest Elisa Bassotti is employee at Eureka Lab 
      Division. Paolo Dossetto is employee at Sciex. The other authors declare that 
      does not exist any economic interest or any conflict of interest.
EDAT- 2020/06/02 06:00
MHDA- 2021/02/02 06:00
CRDT- 2020/06/02 06:00
PHST- 2020/01/17 00:00 [received]
PHST- 2020/05/04 00:00 [revised]
PHST- 2020/05/05 00:00 [accepted]
PHST- 2020/06/02 06:00 [pubmed]
PHST- 2021/02/02 06:00 [medline]
PHST- 2020/06/02 06:00 [entrez]
AID - S0379-0738(20)30192-4 [pii]
AID - 10.1016/j.forsciint.2020.110330 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2020 Jul;312:110330. doi: 10.1016/j.forsciint.2020.110330. Epub 
      2020 May 12.