PMID- 3248124
OWN - NLM
STAT- MEDLINE
DCOM- 19890615
LR  - 20031114
IS  - 0232-766X (Print)
IS  - 0232-766X (Linking)
VI  - 47
IP  - 9
DP  - 1988
TI  - Metabolism of D-proline beta-casomorphin derivatives in the rat brain.
PG  - 865-9
AB  - The aim of the present study was to evaluate the metabolic breakdown of two 
      biologically active derivatives of beta-casomorphin (beta CM)-i.e. 
      D-proline4-beta CM (D-Pro4-beta CM) and des-tyrosine 1-D-proline4-beta CM 
      (DT-D-Pro4-beta CM)- in the rat brain. After intracerebroventricular (icv.) 
      administration of 0.78 nmoles of both [3H]D-Pro4-beta CM and [3H]DT-D-Pro4-beta 
      CM, the concentration of the intact peptides and their metabolites was estimated 
      in brain stem and corpus striatum. Both peptides were degraded in brain tissue 
      forming a common metabolite. Phe-D-Pro-Gly. The metabolic half-lives of 
      DT-D-Pro4-beta CM in brain stem and c. striatum were 22.6 min and 28.6 min, 
      whereas those of D-Pro4-beta CM were 7.8 min and 8.2 min, respectively. According 
      to both half-lives of the intact peptide and the kinetics of the formation of the 
      stable metabolite Phe-D-Pro-Gly. DT-D-Pro4-beta CM seemed to be more resistant to 
      biological degradation in brain tissue than D-Pro4-beta CM. In the case of 
      D-Pro4-beta CM, the dipeptidylpeptidase IV (DP IV) (EC 3.4.14.-) is presumed to 
      be the cardinal enzyme for the breakdown. Since the degradation of these peptides 
      in brain tissue results in the unique metabolite Phe-D-Pro-Gly, the differences 
      in the pattern of biological activities between D-Pro4-beta CM and DT-D-Pro4-beta 
      CM may be due to the action of the corresponding intact peptide.
FAU - Stark, H
AU  - Stark H
AD  - Institute of Neurobiology and Brain Research, Academy of Sciences, Magdeburg, 
      DDR.
FAU - Lossner, B
AU  - Lossner B
FAU - Matthies, H
AU  - Matthies H
FAU - Hartrodt, B
AU  - Hartrodt B
FAU - Born, I
AU  - Born I
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Biomed Biochim Acta
JT  - Biomedica biochimica acta
JID - 8304435
RN  - 0 (Endorphins)
RN  - 0 (Peptide Fragments)
RN  - 72122-63-5 (beta-casomorphin 5)
RN  - 82289-41-6 (beta-casomorphin, des-Tyr-)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain Stem/metabolism
MH  - Cerebral Ventricles/metabolism
MH  - Corpus Striatum/metabolism
MH  - Endorphins/*metabolism
MH  - Kinetics
MH  - Male
MH  - *Peptide Fragments
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PST - ppublish
SO  - Biomed Biochim Acta. 1988;47(9):865-9.