PMID- 32481886
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200602
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 2
IP  - 2
DP  - 2014 Feb 23
TI  - The synergistic effect of a hybrid graphene oxide-chitosan system and biomimetic 
      mineralization on osteoblast functions.
PG  - 264-274
LID - 10.1039/c3bm60192g [doi]
AB  - Graphene oxide and chitosan are promising materials for tissue regeneration. The 
      present study explores a novel biomimetic mineralization route employing a 
      graphene oxide (GO)-chitosan (CS) conjugate as a template material for the 
      biomineralization of hydroxyapatite (HAP). Structural and morphological studies 
      involving X-ray diffraction, Fourier transform infrared spectroscopy, and 
      electron microscopy indicated that extensive mineralization occurred in the CS-GO 
      conjugate system because of strong electrostatic interactions between the 
      functional groups (carboxyl groups of GO and amino groups of CS) and calcium ions 
      in the simulated body fluid (SBF). The combination of chitosan-graphene oxide 
      conjugate and biomineralization was advantageous in favorably modulating cellular 
      activity (osteoblast functions: cell attachment, proliferation, actin, vinculin 
      and fibronectin expression). It is concluded that biomineralized hydroxyapatite 
      in the HAP-CS-GO system induced homogeneous spatial osteoblastic cell growth and 
      quantitatively (e.g. area) and qualitatively (e.g. mineral-to-matrix ratio) 
      increased mineralization in relation to the HAP-GO system. The data underscore 
      that covalent linkage of HAP to chitosan influences osteoblastic cell 
      differentiation, mineralization, and cell growth. The proposed system and the 
      revelation of fundamental insights merit consideration in tissue engineering.
FAU - Depan, D
AU  - Depan D
AD  - Biomaterials and Biomedical Engineering Research Laboratory, Center for 
      Structural and Functional Materials, Institute of Materials Research and 
      Innovation, University of Louisiana at Lafayette, P.O. Box 44130, Lafayette, LA 
      70504-4130, USA. dmisra@louisiana.edu.
FAU - Pesacreta, T C
AU  - Pesacreta TC
FAU - Misra, R D K
AU  - Misra RDK
LA  - eng
PT  - Journal Article
DEP - 20131030
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
SB  - IM
EDAT- 2014/02/23 00:00
MHDA- 2014/02/23 00:01
CRDT- 2020/06/03 06:00
PHST- 2020/06/03 06:00 [entrez]
PHST- 2014/02/23 00:00 [pubmed]
PHST- 2014/02/23 00:01 [medline]
AID - 10.1039/c3bm60192g [doi]
PST - ppublish
SO  - Biomater Sci. 2014 Feb 23;2(2):264-274. doi: 10.1039/c3bm60192g. Epub 2013 Oct 
      30.