PMID- 32484231
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 1941-7225 (Electronic)
IS  - 0895-7061 (Linking)
VI  - 33
IP  - 12
DP  - 2020 Dec 31
TI  - Hyperhomocysteinemia-Induced Oxidative Stress Aggravates Renal Damage in 
      Hypertensive Rats.
PG  - 1127-1135
LID - 10.1093/ajh/hpaa086 [doi]
AB  - BACKGROUND: Hyperhomocysteinemia (HHcy) plays a synergistic role with 
      hypertension in vascular injury; however, the relationship between HHcy and 
      hypertension in renal injury remains unclear. Here, we sought to evaluate the 
      relationship between HHcy and hypertension in the context of renal injury and to 
      elucidate the mechanism of action underlying this relationship. METHODS: Wistar 
      Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) were randomized into 
      WKY, WKY + HHcy, SHR, and SHR + HHcy groups. Blood pressure, plasma homocysteine, 
      serum malondialdehyde (MDA), serum superoxide dismutase (SOD), urinary albumin 
      creatinine ratio (UACR), and glomerular filtration rate (GFR) were measured. 
      Renal histopathology and expression levels of NOX2, NOX4, and nephrin in the 
      kidneys were examined. RESULTS: The WKY + HHcy and SHR groups exhibited lower 
      serum SOD and GFR levels, relative to the WKY group, along with higher levels of 
      both serum MDA and UACR. Higher mRNA and protein expression levels of NOX2 and 
      NOX4, along with lower expression levels of nephrin, were observed in the kidneys 
      of WKY + HHcy and SHR rats, relative to WKY controls, respectively. Similar 
      effects were observed in the SHR + HHcy group, relative to the SHR group and WKY 
      + HHcy group, respectively. Periodic acid-Schiff staining showed an increase in 
      the glomerular extracellular matrix in the WKY + HHcy and SHR + HHcy groups 
      compared with their respective controls. CONCLUSIONS: HHcy appears to 
      synergistically increase hypertensive renal damage by enhancing oxidative stress.
CI  - (c) American Journal of Hypertension, Ltd 2020. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Gao, Ning
AU  - Gao N
AD  - Department of Cardiology, The First Affiliated Hospital of Shandong First Medical 
      University, Jinan, Shandong, China.
FAU - Zhang, Yuzhen
AU  - Zhang Y
AD  - Department of Neurosurgery, The First Affiliated Hospital of Shandong First 
      Medical University, Jinan, Shandong, China.
FAU - Li, Li
AU  - Li L
AD  - Department of Clinical Nutrition, the People's Hospital of Zhangqiu, Jinan, 
      Shandong, China.
FAU - Lei, Lei
AU  - Lei L
AD  - Department of Cardiology, The First Affiliated Hospital of Shandong First Medical 
      University, Jinan, Shandong, China.
FAU - Cao, Ping
AU  - Cao P
AD  - Department of Geriatric Medicine, Tai'an City Central Hospital, Tai'an, Shandong, 
      China.
FAU - Zhao, Xuan
AU  - Zhao X
AD  - Department of Cardiovascular Medicine, People's Hospital of Dongying, Dongying, 
      Shandong, China.
FAU - Lin, Lin
AU  - Lin L
AD  - Department of Cardiology, The First Affiliated Hospital of Shandong First Medical 
      University, Jinan, Shandong, China.
FAU - Xu, Rui
AU  - Xu R
AD  - Department of Cardiology, The First Affiliated Hospital of Shandong First Medical 
      University, Jinan, Shandong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Hypertens
JT  - American journal of hypertension
JID - 8803676
RN  - 0 (Membrane Proteins)
RN  - 0 (nephrin)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - AYI8EX34EU (Creatinine)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.6.3.- (Cybb protein, rat)
RN  - EC 1.6.3.- (NADPH Oxidase 2)
RN  - EC 1.6.3.- (NADPH Oxidase 4)
RN  - EC 1.6.3.- (Nox4 protein, rat)
SB  - IM
MH  - Albuminuria
MH  - Animals
MH  - Blood Pressure
MH  - Creatinine/urine
MH  - Glomerular Filtration Rate
MH  - Homocysteine/metabolism
MH  - Hyperhomocysteinemia/genetics/*metabolism/pathology
MH  - Hypertension/genetics/*metabolism/pathology
MH  - Kidney/*metabolism/pathology
MH  - Malondialdehyde/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - NADPH Oxidase 2/genetics/metabolism
MH  - NADPH Oxidase 4/genetics/metabolism
MH  - *Oxidative Stress
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Renal Insufficiency/genetics/*metabolism/pathology
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - HHcy
OT  - NOX2
OT  - NOX4
OT  - blood pressure
OT  - glomerular injury
OT  - hypertension
OT  - nephrin
EDAT- 2020/06/03 06:00
MHDA- 2021/11/09 06:00
CRDT- 2020/06/03 06:00
PHST- 2019/11/11 00:00 [received]
PHST- 2020/04/03 00:00 [revised]
PHST- 2020/05/25 00:00 [accepted]
PHST- 2020/06/03 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2020/06/03 06:00 [entrez]
AID - 5850246 [pii]
AID - 10.1093/ajh/hpaa086 [doi]
PST - ppublish
SO  - Am J Hypertens. 2020 Dec 31;33(12):1127-1135. doi: 10.1093/ajh/hpaa086.