PMID- 32485953
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 11
DP  - 2020 May 29
TI  - Syndecan-1 Facilitates the Human Mesenchymal Stem Cell Osteo-Adipogenic Balance.
LID - 10.3390/ijms21113884 [doi]
LID - 3884
AB  - Bone marrow-derived human mesenchymal stems cells (hMSCs) are precursors to 
      adipocyte and osteoblast lineage cells. Dysregulation of the osteo-adipogenic 
      balance has been implicated in pathological conditions involving bone loss. 
      Heparan sulfate proteoglycans (HSPGs) such as cell membrane-bound syndecans 
      (SDCs) and glypicans (GPCs) mediate hMSC lineage differentiation and with 
      syndecan-1 (SDC-1) reported in both adipogenesis and osteogenesis, these 
      macromolecules are potential regulators of the osteo-adipogenic balance. Here, we 
      disrupted the HSPG profile in primary hMSC cultures via temporal knockdown (KD) 
      of SDC-1 using RNA interference (RNAi) in undifferentiated, osteogenic and 
      adipogenic differentiated hMSCs. SDC-1 KD cultures were examined for osteogenic 
      and adipogenic lineage markers along with changes in HSPG profile and common 
      signalling pathways implicated in hMSC lineage fate. Undifferentiated hMSC SDC-1 
      KD cultures exhibited a pro-adipogenic phenotype with subsequent osteogenic 
      differentiation demonstrating enhanced maturation of osteoblasts. In cultures 
      where SDC-1 KD was performed following initiation of differentiation, increased 
      adipogenic gene and protein marker expression along with increased Oil Red O 
      staining identified enhanced adipogenesis, with impaired osteogenesis also 
      observed in these cultures. These findings implicate SDC-1 as a facilitator of 
      the hMSC osteo-adipogenic balance during early induction of lineage 
      differentiation.
FAU - Yu, Chieh
AU  - Yu C
AUID- ORCID: 0000-0003-3712-5491
AD  - Queensland University of Technology, Genomics Research Centre, School of 
      Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
FAU - Peall, Ian W
AU  - Peall IW
AD  - Queensland University of Technology, Genomics Research Centre, School of 
      Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
FAU - Pham, Son H
AU  - Pham SH
AD  - Queensland University of Technology, Genomics Research Centre, School of 
      Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
FAU - Okolicsanyi, Rachel K
AU  - Okolicsanyi RK
AD  - Queensland University of Technology, Genomics Research Centre, School of 
      Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
FAU - Griffiths, Lyn R
AU  - Griffiths LR
AUID- ORCID: 0000-0002-6774-5475
AD  - Queensland University of Technology, Genomics Research Centre, School of 
      Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
FAU - Haupt, Larisa M
AU  - Haupt LM
AUID- ORCID: 0000-0002-7735-8110
AD  - Queensland University of Technology, Genomics Research Centre, School of 
      Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
LA  - eng
GR  - CY/Australian Postgraduate Award/
GR  - Ph.D. Enabling Grant, Haupt/School of Biomedical Sciences, QUT/
PT  - Journal Article
DEP - 20200529
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (SDC1 protein, human)
RN  - 0 (Syndecan-1)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Adipogenesis
MH  - Adiposity
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cell Membrane/metabolism
MH  - Cell Proliferation
MH  - Extracellular Matrix Proteins/metabolism
MH  - Heparan Sulfate Proteoglycans/chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Osteoblasts/cytology
MH  - Osteocytes/*cytology
MH  - Osteogenesis
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Syndecan-1/*metabolism
MH  - Up-Regulation
PMC - PMC7312587
OTO - NOTNLM
OT  - adipogenesis
OT  - heparan sulfate proteoglycans
OT  - lineage fate
OT  - mesenchymal stem cells
OT  - osteogenesis
OT  - syndecan-1
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2020/06/04 06:00
MHDA- 2021/03/31 06:00
PMCR- 2020/06/01
CRDT- 2020/06/04 06:00
PHST- 2020/04/29 00:00 [received]
PHST- 2020/05/27 00:00 [revised]
PHST- 2020/05/27 00:00 [accepted]
PHST- 2020/06/04 06:00 [entrez]
PHST- 2020/06/04 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - ijms21113884 [pii]
AID - ijms-21-03884 [pii]
AID - 10.3390/ijms21113884 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 May 29;21(11):3884. doi: 10.3390/ijms21113884.