PMID- 32486094
OWN - NLM
STAT- MEDLINE
DCOM- 20210301
LR  - 20210301
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 12
IP  - 6
DP  - 2020 May 29
TI  - Annona muricata L.-Derived Polysaccharides as a Potential Adjuvant to a Dendritic 
      Cell-Based Vaccine in a Thymoma-Bearing Model.
LID - 10.3390/nu12061602 [doi]
LID - 1602
AB  - Dendritic cells (DCs) are powerful antigen-presenting cells that are often used 
      to evaluate adjuvants, particularly for adjuvant selection for various vaccines. 
      Here, polysaccharides (named ALP) isolated from leaves of Annona muricata L., 
      which are used in traditional medicine such as for bacterial infections and 
      inflammatory diseases, were evaluated as an adjuvant candidate that can induce 
      anti-tumor activity. We first confirmed the phenotypic (surface molecules, 
      cytokines, antigen uptake, and antigen-presenting ability) and functional 
      alterations (T cell proliferation/activation) of DCs in vitro. We also confirmed 
      the adjuvant effect by evaluating anti-tumor activity and immunity using an 
      ALP-treated DC-immunized mouse model. ALP functionally induced DC maturation by 
      up-regulating the secretion of Th1-polarizing pro-inflammatory cytokines, the 
      expression of surface molecules, and antigen-presenting ability. ALP triggered DC 
      maturation, which is dependent on the activation of the MAPK and NF-kappaB signaling 
      pathways. ALP-activated DCs showed an ample capacity to differentiate naive T 
      cells to Th1 and activated CD8(+) T cells effectively. The systemic 
      administration of DCs that pulse ALP and ovalbumin peptides strongly increased 
      cytotoxic T lymphocyte (CTL) activity (by 9.5% compared to that in the control 
      vaccine groups), the generation of CD107a-producing multifunctional T cells, and 
      Th1-mediated humoral immunity, and caused a significant reduction (increased 
      protection by 29% over that in control vaccine groups) in tumor growth. ALP, 
      which triggers the Th1 and CTL response, provides a basis for a new adjuvant for 
      various vaccines.
FAU - Kim, Woo Sik
AU  - Kim WS
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup 580-185, Korea.
FAU - Han, Jeong Moo
AU  - Han JM
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup 580-185, Korea.
AD  - Department of Biotechnology, College of Life Science and Biotechnology, Korea 
      University, Seoul 136-701, Korea.
FAU - Song, Ha-Yeon
AU  - Song HY
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup 580-185, Korea.
AD  - Department of Biotechnology, College of Life Science and Biotechnology, Korea 
      University, Seoul 136-701, Korea.
FAU - Byun, Eui-Hong
AU  - Byun EH
AD  - Department of Food Science and Technology, Kongju National University, Yesan 
      340-800, Korea.
FAU - Lim, Seung-Taik
AU  - Lim ST
AD  - Department of Biotechnology, College of Life Science and Biotechnology, Korea 
      University, Seoul 136-701, Korea.
FAU - Byun, Eui-Baek
AU  - Byun EB
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup 580-185, Korea.
LA  - eng
GR  - NRF-2019R1C1C1002484/This work was supported by the Basic science Research 
      Program through the National Research Foundation of Korea (NRF) funded by the 
      Ministry of Science, ICT and Future Planning/
PT  - Journal Article
DEP - 20200529
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cancer Vaccines)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Polysaccharides)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - Annonaceae/*chemistry
MH  - Antigen Presentation
MH  - *Cancer Vaccines
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/metabolism
MH  - *Polysaccharides/immunology/isolation & purification/pharmacology
MH  - Signal Transduction/drug effects/immunology
MH  - T-Lymphocytes/immunology
MH  - Thymoma/*immunology/therapy
MH  - Thymus Neoplasms/*immunology/therapy
PMC - PMC7352220
OTO - NOTNLM
OT  - Annona muricata L.
OT  - Th1
OT  - cytotoxic T lymphocyte
OT  - dendritic cells
OT  - multifunctional T cells
OT  - polysaccharide
OT  - vaccine adjuvant
COIS- The authors declare no conflict of interest.
EDAT- 2020/06/04 06:00
MHDA- 2021/03/02 06:00
PMCR- 2020/06/01
CRDT- 2020/06/04 06:00
PHST- 2020/04/28 00:00 [received]
PHST- 2020/05/23 00:00 [revised]
PHST- 2020/05/27 00:00 [accepted]
PHST- 2020/06/04 06:00 [entrez]
PHST- 2020/06/04 06:00 [pubmed]
PHST- 2021/03/02 06:00 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - nu12061602 [pii]
AID - nutrients-12-01602 [pii]
AID - 10.3390/nu12061602 [doi]
PST - epublish
SO  - Nutrients. 2020 May 29;12(6):1602. doi: 10.3390/nu12061602.