PMID- 32487167
OWN - NLM
STAT- MEDLINE
DCOM- 20210217
LR  - 20230712
IS  - 1476-4598 (Electronic)
IS  - 1476-4598 (Linking)
VI  - 19
IP  - 1
DP  - 2020 Jun 2
TI  - Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by 
      modulating the let-7c-5p/PBX3 axis.
PG  - 99
LID - 10.1186/s12943-020-01215-4 [doi]
LID - 99
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor 
      of the head and neck. LSCC patients have seriously impaired vocal, respiratory, 
      and swallowing functions with poor prognosis. Circular RNA (circRNA) has 
      attracted great attention in cancer research. However, the expression patterns 
      and roles of circRNAs in LSCC remain largely unknown. METHODS: RNA sequencing was 
      performed on 57 pairs of LSCC and matched adjacent normal mucosa tissues to 
      construct circRNA, miRNA, and mRNA expression profiles. RT-PCR, qPCR, Sanger 
      sequencing, and FISH were undertaken to study the expression, localization, and 
      clinical significance of circCORO1C in LSCC tissues and cells. The functions of 
      circCORO1C in LSCC were investigated by RNAi-mediated knockdown, proliferation 
      analysis, EdU staining, colony formation assay, Transwell assay, and apoptosis 
      analysis. The regulatory mechanisms among circCORO1C, let-7c-5p, and PBX3 were 
      investigated by luciferase assay, RNA immunoprecipitation, western blotting, and 
      immunohistochemistry. RESULTS: circCORO1C was highly expressed in LSCC tissues 
      and cells, and this high expression was closely associated with the malignant 
      progression and poor prognosis of LSCC. Knockdown of circCORO1C inhibited the 
      proliferation, migration, invasion, and in vivo tumorigenesis of LSCC cells. 
      Mechanistic studies revealed that circCORO1C competitively bound to let-7c-5p and 
      prevented it from decreasing the level of PBX3, which promoted the 
      epithelial-mesenchymal transition and finally facilitated the malignant 
      progression of LSCC. CONCLUSIONS: circCORO1C has an oncogenic role in LSCC 
      progression and may serve as a novel target for LSCC therapy. circCORO1C 
      expression has the potential to serve as a novel diagnostic and prognostic 
      biomarker for LSCC detection.
FAU - Wu, Yongyan
AU  - Wu Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Department of Biochemistry & Molecular Biology, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Zhang, Yuliang
AU  - Zhang Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
FAU - Zheng, Xiwang
AU  - Zheng X
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
FAU - Dai, Fengsheng
AU  - Dai F
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Lu, Yan
AU  - Lu Y
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Jinzhou 
      Medical University, Jinzhou, 121001, Liaoning, People's Republic of China.
FAU - Dai, Li
AU  - Dai L
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Niu, Min
AU  - Niu M
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
FAU - Guo, Huina
AU  - Guo H
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
FAU - Li, Wenqi
AU  - Li W
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Xue, Xuting
AU  - Xue X
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
FAU - Bo, Yunfeng
AU  - Bo Y
AD  - Department of Pathology, Shanxi Cancer Hospital, Shanxi Medical University, 
      Taiyuan, 030013, Shanxi, People's Republic of China.
FAU - Guo, Yujia
AU  - Guo Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
FAU - Qin, Jiangbo
AU  - Qin J
AD  - Department of Otolaryngology Head & Neck Surgery, Heping Hospital Affiliated to 
      Changzhi Medical College, Changzhi, 046000, Shanxi, People's Republic of China.
FAU - Qin, Yixiao
AU  - Qin Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China.
AD  - Department of Cell Biology and Genetics, Basic Medical School of Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
AD  - Department of Physiology, Shanxi Medical University, Taiyuan, 030001, Shanxi, 
      People's Republic of China.
FAU - Yang, Tao
AU  - Yang T
AD  - Department of Biochemistry & Molecular Biology, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Li, Li
AU  - Li L
AD  - Department of Cell Biology and Genetics, Basic Medical School of Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China.
FAU - Zhang, Linshi
AU  - Zhang L
AD  - Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated 
      Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, 
      People's Republic of China.
FAU - Hou, Rui
AU  - Hou R
AD  - Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for 
      Medical Research, the University of Western Australia, PO Box 7214, 6 Verdun 
      Street, Nedlands, Perth, Western Australia, 6009, Australia.
FAU - Wen, Shuxin
AU  - Wen S
AD  - General Hospital, Shenzhen University, Shenzhen, 518055, Guangdong, People's 
      Republic of China.
FAU - An, Changming
AU  - An C
AD  - Department of Head and Neck Surgery, Cancer Hospital, National Cancer Center, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 
      100021, People's Republic of China. anchangming@cicams.ac.cn.
FAU - Li, Huizheng
AU  - Li H
AD  - Department of Otolaryngology Head & Neck Surgery, Dalian Municipal Friendship 
      Hospital, Dalian Medical University, Dalian, 116100, Liaoning, People's Republic 
      of China. huizhengli2004@163.com.
FAU - Xu, Wei
AU  - Xu W
AD  - Shandong Provincial ENT Hospital Affiliated to Shandong University, Jinan, 
      250022, Shandong, People's Republic of China. xuwhns@126.com.
AD  - Shandong Provincial Institute of Otolaryngology, Jinan, 250022, Shandong, 
      People's Republic of China. xuwhns@126.com.
AD  - Key Laboratory of Otolaryngology, Ministry of Health, Shandong University, Jinan, 
      250022, Shandong, People's Republic of China. xuwhns@126.com.
FAU - Gao, Wei
AU  - Gao W
AUID- ORCID: 0000-0001-7836-2851
AD  - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China. 
      gaoweisxent@sxent.org.
AD  - Shanxi Province Clinical Medical Research Center for Precision Medicine of Head 
      and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 
      030001, Shanxi, People's Republic of China. gaoweisxent@sxent.org.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. 
      gaoweisxent@sxent.org.
AD  - Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China. 
      gaoweisxent@sxent.org.
AD  - Department of Cell Biology and Genetics, Basic Medical School of Shanxi Medical 
      University, Taiyuan, 030001, Shanxi, People's Republic of China. 
      gaoweisxent@sxent.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200602
PL  - England
TA  - Mol Cancer
JT  - Molecular cancer
JID - 101147698
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Circular)
RN  - 0 (mirnlet7 microRNA, human)
RN  - 145420-64-0 (coronin proteins)
RN  - 146150-81-4 (proto-oncogene protein Pbx3)
SB  - IM
EIN - Mol Cancer. 2023 Mar 15;22(1):51. PMID: 36927346
EIN - Mol Cancer. 2023 Jul 12;22(1):109. PMID: 37438789
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Gene Expression Regulation, Neoplastic
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*genetics
MH  - Microfilament Proteins/*genetics
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - RNA, Circular/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/metabolism/*pathology
MH  - Tumor Cells, Cultured
MH  - Xenograft Model Antitumor Assays
PMC - PMC7265647
OTO - NOTNLM
OT  - Epithelial-mesenchymal transition
OT  - Laryngeal squamous cell carcinoma
OT  - Let-7c-5p
OT  - Metastasis
OT  - PBX3
OT  - circCORO1C
COIS- The authors declare that they have no competing interests.
EDAT- 2020/06/04 06:00
MHDA- 2021/02/18 06:00
PMCR- 2020/06/02
CRDT- 2020/06/04 06:00
PHST- 2020/01/20 00:00 [received]
PHST- 2020/05/11 00:00 [accepted]
PHST- 2020/06/04 06:00 [entrez]
PHST- 2020/06/04 06:00 [pubmed]
PHST- 2021/02/18 06:00 [medline]
PHST- 2020/06/02 00:00 [pmc-release]
AID - 10.1186/s12943-020-01215-4 [pii]
AID - 1215 [pii]
AID - 10.1186/s12943-020-01215-4 [doi]
PST - epublish
SO  - Mol Cancer. 2020 Jun 2;19(1):99. doi: 10.1186/s12943-020-01215-4.