PMID- 32488904
OWN - NLM
STAT- MEDLINE
DCOM- 20201208
LR  - 20201214
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 31
IP  - 6
DP  - 2020 Nov
TI  - Qualitative and quantitative determination of steroidal saponins in Trillium 
      govanianum by UHPLC-QTOF-MS/MS and UHPLC-ELSD.
PG  - 861-873
LID - 10.1002/pca.2951 [doi]
AB  - INTRODUCTION: Trillium govanianum (Nag Chhatri and Teen Patra) is traditionally 
      used for curing joint pains, wounds, and sexual disorders. Steroidal saponins are 
      the main active components of this species. However, only a small amount of 
      information is available about steroidal saponins of this plant. OBJECTIVE: To 
      develop an ultra-high-performance liquid chromatography-quadrupole time of flight 
      tandem mass spectrometry (UHPLC-QTOF-MS/MS) and ultra-high-performance liquid 
      chromatography-evaporative light scattering detector (UHPLC-ELSD) methods for the 
      qualitative and quantitative determination of steroidal saponins in T. 
      govanianum. METHOD: The dried rhizomes of T. govanianum (100 mg) were extracted 
      with ethanol-water (80:20, 10 mL) by ultrasonic treatment for 30 min at 40 degrees C. The 
      prepared sample was analysed by UHPLC-QTOF-MS/MS and UHPLC-ELSD for the 
      qualitative and quantitative determination of steroidal saponins. RESULT: A total 
      of 24 saponins were identified using UHPLC-QTOF-MS/MS; seven of them were 
      characterised by comparing with standards. Furthermore, five saponins [govanoside 
      B (2), protodioscin (6), pennogenin tetraglycosides (11), borassoside E (21) and 
      borassoside D (24)] were quantified using UHPLC-ELSD method in different extracts 
      and fractions of T. govanianum. The method showed good linearity (R(2) >/= 0.993), 
      limit of detection (0.92-4.09 mug/mL), limit of quantification (3.1-13.5 mug/mL), 
      precision [intra-day relative standard deviations (RSDs) < 4.3% and inter-day 
      RSDs < 5.5%], and accuracy (84.0-110.3%). This is the first report on the 
      quantification of 2, 6, 11, 21 and 24 in T. govanianum. CONCLUSION: The present 
      study provides an efficient analytical method for the identification and 
      quantification of steroidal saponins and will be helpful for the quality 
      evaluation of T. govanianum.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Singh, Prithvi Pal
AU  - Singh PP
AD  - Natural Product Chemistry and Process Development Division and AcSIR, 
      CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 
      India.
FAU - Bora, Prateek Singh
AU  - Bora PS
AD  - Natural Product Chemistry and Process Development Division and AcSIR, 
      CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 
      India.
FAU - Suresh, Patil Shivprasad
AU  - Suresh PS
AD  - Natural Product Chemistry and Process Development Division and AcSIR, 
      CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 
      India.
FAU - Bhatt, Vinod
AU  - Bhatt V
AD  - Natural Product Chemistry and Process Development Division and AcSIR, 
      CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 
      India.
FAU - Sharma, Upendra
AU  - Sharma U
AUID- ORCID: 0000-0002-7693-8690
AD  - Natural Product Chemistry and Process Development Division and AcSIR, 
      CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20200603
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Saponins)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Rhizome
MH  - *Saponins
MH  - Tandem Mass Spectrometry
MH  - *Trillium
OTO - NOTNLM
OT  - Trillium govanianum
OT  - UHPLC-ELSD
OT  - UHPLC-QTOF-MS/MS
OT  - steroidal saponin
EDAT- 2020/06/04 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/06/04 06:00
PHST- 2020/02/05 00:00 [received]
PHST- 2020/04/30 00:00 [revised]
PHST- 2020/05/04 00:00 [accepted]
PHST- 2020/06/04 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/06/04 06:00 [entrez]
AID - 10.1002/pca.2951 [doi]
PST - ppublish
SO  - Phytochem Anal. 2020 Nov;31(6):861-873. doi: 10.1002/pca.2951. Epub 2020 Jun 3.