PMID- 32492957
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 9
IP  - 6
DP  - 2020 Jun 1
TI  - AOP1, a New Live Cell Assay for the Direct and Quantitative Measure of 
      Intracellular Antioxidant Effects.
LID - 10.3390/antiox9060471 [doi]
LID - 471
AB  - Taking advantage of Light Up Cell System (LUCS) technology, which allows for fine 
      monitoring of reactive oxygen species (ROS) production inside live cells, a new 
      assay called Anti Oxidant Power 1 (AOP1) was developed to specifically measure 
      ROS and/or free-radical scavenging effects inside living cells. This method is 
      quantitative and EC(50)s obtained from AOP1 dose-response experiments were 
      determined in order to classify the intracellular antioxidant efficacy of 15 well 
      known antioxidant compounds with different hydrophilic properties. Six of them 
      (epigallocatechin gallate, quercetin, butylated hydroxyanisole (BHA), butylated 
      hydroxytoluene (BHT), ethoxyquin, resveratrol) gave EC(50)s in the range of 7-64 
      muM, four (Trolox, catechin, epicatechin, EUK134) in the range of 0.14 to 1 mM, 
      and 5 (sulforaphane, astaxanthin, alpha- and gamma-tocopherols, vitamin E acetate) showed 
      only partial or no effect. Interestingly, effects with measurable EC(50)s were 
      observed for compounds with hydrophilic properties (LogP </= 5.3), while all 
      antioxidants known to act at the plasma membrane level (LogP >/= 10.3) had partial 
      or no effect. Sulforaphane, a hydrophilic but strict Keap1/Nrf2 pathway enhancer, 
      did not show any effect either. Importantly, AOP1 assay captures both antioxidant 
      and prooxidant effects. Taken together, these results led us to the conclusion 
      that AOP1 assay measures antioxidant effect of compounds that selectively enter 
      the cell, and act as free radical scavengers in the cytosol and/or nucleus level.
FAU - Gironde, Camille
AU  - Gironde C
AD  - AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 31031 Toulouse, France.
FAU - Rigal, Mylene
AU  - Rigal M
AD  - AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 31031 Toulouse, France.
FAU - Dufour, Cecile
AU  - Dufour C
AD  - AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 31031 Toulouse, France.
FAU - Furger, Christophe
AU  - Furger C
AD  - AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 31031 Toulouse, France.
LA  - eng
PT  - Journal Article
DEP - 20200601
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC7346189
OTO - NOTNLM
OT  - antioxidant assay
OT  - cellular antioxidant effect
OT  - free radicals
OT  - live cell assay
OT  - radical scavenging
OT  - reactive oxygen species (ROS)
COIS- Dr Christophe Furger declares competing financial interest due to patent 
      ownership on LUCS method. Authors are employees of Anti Oxidant Power - AOP, the 
      start-up company which operates the patent.
EDAT- 2020/06/05 06:00
MHDA- 2020/06/05 06:01
PMCR- 2020/06/01
CRDT- 2020/06/05 06:00
PHST- 2020/04/20 00:00 [received]
PHST- 2020/05/20 00:00 [revised]
PHST- 2020/05/27 00:00 [accepted]
PHST- 2020/06/05 06:00 [entrez]
PHST- 2020/06/05 06:00 [pubmed]
PHST- 2020/06/05 06:01 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - antiox9060471 [pii]
AID - antioxidants-09-00471 [pii]
AID - 10.3390/antiox9060471 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2020 Jun 1;9(6):471. doi: 10.3390/antiox9060471.