PMID- 32493389 OWN - NLM STAT- MEDLINE DCOM- 20210217 LR - 20210217 IS - 1476-4598 (Electronic) IS - 1476-4598 (Linking) VI - 19 IP - 1 DP - 2020 Jun 3 TI - Circular RNA circSATB2 promotes progression of non-small cell lung cancer cells. PG - 101 LID - 10.1186/s12943-020-01221-6 [doi] LID - 101 AB - BACKGROUND: Lung cancer has high morbidity and mortality worldwide with non-small cell lung cancer (NSCLC) accounting for 85% of the cases. Therapies for lung cancer have relatively poor outcomes and further improvements are required. Circular RNAs have been reported to participate in the occurrence and progression of cancer. Information on the functions and mechanism of circRNAs in lung cancer is limited and needs more exploration. METHODS: We detected expression of genes and proteins by qPCR and western blot. Function of circSATB2 was investigated using RNA interference and overexpression assays. Location of circSATB2 was assessed by fluorescence in situ hybridization (FISH). Interaction of circSATB2, miR-326 and FSCN1 was confirmed by dual-luciferase reporter assay. RESULTS: Data from the investigation showed that circSATB2 was highly expressed in NSCLC cells and tissues. circSATB2 positively regulated fascin homolog 1, actin-bundling protein 1 (FSCN1) expression via miR-326 in lung cancer cells. Furthermore, circSATB2 can be transferred by exosomes and promote the proliferation, migration and invasion of NSCLC cells, as well as induce abnormal proliferation in normal human bronchial epithelial cells. Also, circSATB2 was highly expressed in serumal exosomes from lung cancer patients with high sensitivity and specificity for clinical detection and was related to lung cancer metastasis. CONCLUSIONS: circSATB2 participated in the progression of NSCLC and was differentially expressed in lung cancer tissue and serumal exosomes. circSATB2 may be potential biomarker for the diagnosis of NSCLC. FAU - Zhang, Nan AU - Zhang N AD - State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Nan, Aruo AU - Nan A AD - State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Chen, Lijian AU - Chen L AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Li, Xin AU - Li X AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Jia, Yangyang AU - Jia Y AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Qiu, Miaoyun AU - Qiu M AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Dai, Xin AU - Dai X AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Zhou, Hanyu AU - Zhou H AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Zhu, Jialu AU - Zhu J AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Zhang, Han AU - Zhang H AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. FAU - Jiang, Yiguo AU - Jiang Y AUID- ORCID: 0000-0002-9499-2594 AD - State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. jiangyiguo@vip.163.com. AD - Institute for Chemical Carcinogenesis, Guangzhou Medical University, Xinzao, Panyu District, Guangzhou, 511436, People's Republic of China. jiangyiguo@vip.163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200603 PL - England TA - Mol Cancer JT - Molecular cancer JID - 101147698 RN - 0 (Biomarkers, Tumor) RN - 0 (Carrier Proteins) RN - 0 (FSCN1 protein, human) RN - 0 (MIRN326 microRNA, human) RN - 0 (Matrix Attachment Region Binding Proteins) RN - 0 (MicroRNAs) RN - 0 (Microfilament Proteins) RN - 0 (RNA, Circular) RN - 0 (SATB2 protein, human) RN - 0 (Transcription Factors) SB - IM MH - Apoptosis MH - Biomarkers, Tumor/genetics/*metabolism MH - Carcinoma, Non-Small-Cell Lung/genetics/metabolism/*pathology MH - Carrier Proteins/genetics/*metabolism MH - Cell Movement MH - Cell Proliferation MH - Disease Progression MH - Gene Expression Regulation, Neoplastic MH - Humans MH - Lung Neoplasms/genetics/metabolism/*pathology MH - Matrix Attachment Region Binding Proteins/*genetics MH - MicroRNAs/*genetics MH - Microfilament Proteins/genetics/*metabolism MH - Prognosis MH - RNA, Circular/*genetics MH - Transcription Factors/*genetics MH - Tumor Cells, Cultured PMC - PMC7268724 OTO - NOTNLM OT - Exosome OT - Lung cancer OT - Progression OT - circRNA OT - miRNA COIS- The authors have no conflicts of interest to declare. EDAT- 2020/06/05 06:00 MHDA- 2021/02/18 06:00 PMCR- 2020/06/03 CRDT- 2020/06/05 06:00 PHST- 2019/08/26 00:00 [received] PHST- 2020/05/21 00:00 [accepted] PHST- 2020/06/05 06:00 [entrez] PHST- 2020/06/05 06:00 [pubmed] PHST- 2021/02/18 06:00 [medline] PHST- 2020/06/03 00:00 [pmc-release] AID - 10.1186/s12943-020-01221-6 [pii] AID - 1221 [pii] AID - 10.1186/s12943-020-01221-6 [doi] PST - epublish SO - Mol Cancer. 2020 Jun 3;19(1):101. doi: 10.1186/s12943-020-01221-6.