PMID- 32494274
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 1682-024X (Print)
IS  - 1681-715X (Electronic)
IS  - 1681-715X (Linking)
VI  - 36
IP  - 4
DP  - 2020 May-Jun
TI  - Dysregulated epidermal growth factor and tumor growth factor-beta receptor 
      signaling through GFAP-ACTA2 protein interaction in liver fibrosis.
PG  - 782-787
LID - 10.12669/pjms.36.4.1845 [doi]
AB  - OBJECTIVE: Viral hepatitis is associated with high morbidity and mortality. 
      Identification of biological pathways involved in hepatic fibrosis resulting from 
      chronic hepatitis C are essential for better management of patients. Constructing 
      the HCV-human protein interaction network through bioinformatics may enable us to 
      discover diagnostic biological pathways. We investigated to identify dysregulated 
      pathways and gene enrichment based on actin alpha 2 (ACTA2) and glial fibrillar 
      acidic protein (GFAP) interaction network analysis in hepatic fibrosis. METHODS: 
      This is an in-silico study conducted at Ziauddin University from March,2019 to 
      September 2019. Enrichment and protein-protein interaction (PPI) network analysis 
      of the identified proteins: GFAP and ACTA2 along with their mapped gene data sets 
      was performed using FunRich version 3.1.3. RESULTS: Biological pathway grouping 
      showed enrichment of proteins (85.7%) in signalling pathway by epidermal growth 
      factor receptor (EGFR) and Tumor growth factor (TGF)-beta Receptor followed by 
      signaling by PDGF, FGFR and NGF (71.4%) (p < 0.001). SRC, PRKACA, PRKCA and PRKCD 
      were enriched in both EGFR and TGF-beta Signalling pathways. CONCLUSION: EGFR and 
      TGF-beta signalling pathways were enriched in liver fibrosis. SRC, PRKACA, PRKCA 
      and PRKCD were enriched and differentially expressed in both EGFR and TGF-beta 
      signalling pathways.
CI  - Copyright: (c) Pakistan Journal of Medical Sciences.
FAU - Hassan, Sobia
AU  - Hassan S
AD  - Dr. Sobia Hassan, MBBS, M. Phil. Altamash Institute of Dental Medicine, Karachi, 
      Pakistan.
FAU - Zil-E-Rubab
AU  - Zil-E-Rubab
AD  - Dr. Zil-e-Rubab, MBBS, M. Phil, PhD. Ziauddin University Clifton Campus, Karachi, 
      Pakistan.
FAU - Shah, Hussain
AU  - Shah H
AD  - Mr. Hussain Shah, Department of Chemical & Biomolecular Engineering, University 
      of Melbourne, Australia.
FAU - Shawana, Summayya
AU  - Shawana S
AD  - Dr. Summayya Shawana, MBBs, M. Phil. Bahria University Medical & Dental College, 
      Karachi, Pakistan.
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Med Sci
JT  - Pakistan journal of medical sciences
JID - 100913117
PMC - PMC7260937
OTO - NOTNLM
OT  - ACTA2
OT  - GFAP
OT  - Liver fibrosis
OT  - Signalling by EGFR
OT  - TGF-beta receptor Signalling
COIS- Conflicts of interest: The authors declare no conflict of interest with regard to 
      this work
EDAT- 2020/06/05 06:00
MHDA- 2020/06/05 06:01
PMCR- 2020/05/01
CRDT- 2020/06/05 06:00
PHST- 2020/06/05 06:00 [entrez]
PHST- 2020/06/05 06:00 [pubmed]
PHST- 2020/06/05 06:01 [medline]
PHST- 2020/05/01 00:00 [pmc-release]
AID - PJMS-36-782 [pii]
AID - 10.12669/pjms.36.4.1845 [doi]
PST - ppublish
SO  - Pak J Med Sci. 2020 May-Jun;36(4):782-787. doi: 10.12669/pjms.36.4.1845.