PMID- 32494901
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20221207
IS  - 1559-0100 (Electronic)
IS  - 1355-008X (Linking)
VI  - 69
IP  - 3
DP  - 2020 Sep
TI  - Nitric oxide and TNF-alpha are correlates of diabetic retinopathy independent of 
      hs-CRP and HbA1c.
PG  - 536-541
LID - 10.1007/s12020-020-02353-x [doi]
AB  - PURPOSE: Regarding the role of inflammation in progression of diabetes this study 
      was conducted to investigate the association between inflammatory biomarkers such 
      as nitric oxide (NO), tumor necrosis factor alpha (TNF-alpha), and high-sensitivity 
      C-reactive protein (hs-CRP) with the chance of existence of diabetic retinopathy 
      and its progression in patients with diabetes. METHODS: A total of 83 patients 
      with T2DM (Type 2 diabetes mellitus) were divided into three groups of patients 
      with proliferative diabetic retinopathy (PDR), patients with non-proliferative 
      diabetic retinopathy (NPDR) and patients without diabetic retinopathy (NDR) based 
      on ophthalmologic funduscopic examination. Twenty six healthy controls were also 
      enrolled. Blood samples were taken after 12 h of overnight fasting, NO, TNF-alpha, 
      and hs-CRP were measured. Association of the level of these biomarkers with 
      retinopathy was analyzed. RESULTS: The levels of TNF-alpha, NO and hs-CRP were higher 
      among patients with diabetic retinopathy. Multinomial Logistic Regression model 
      showed that TNF-alpha and NO could predict the presence of retinopathy among patients 
      with diabetes when adjusted for hs-CRP, HbA1c, FBS, gender, total cholesterol, 
      triglyceride, HDL, LDL, BMI, and age (respectively OR = 1.76, CI 95% = 1.01-3.02, 
      p = 0.046 and OR = 1.12, CI 95% = 1.05-1.18, p < 0.001); however they could not 
      predict the severity of retinopathy. In ROC analysis AUC for TNFalpha was 0.849 
      (p < 0.001) and for NO was 0.907 (p < 0.001). Serum TNF-alpha level of 7.10 pmol/L 
      could be suggestive of the presence of retinopathy (sensitivity = 92.2% and 
      specificity = 66.0%), also serum NO level of 45.96 mumol/L could be suggestive of 
      the presence of retinopathy (sensitivity = 96.1% and specificity = 86%). 
      CONCLUSIONS: Our results suggest elevated levels of NO and TNF-alpha can be 
      suggestive of diabetic retinopathy.
FAU - Khaloo, Pegah
AU  - Khaloo P
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Qahremani, Reihane
AU  - Qahremani R
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Rabizadeh, Soghra
AU  - Rabizadeh S
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Omidi, Mohammad
AU  - Omidi M
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Rajab, Armin
AU  - Rajab A
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Heidari, Firouzeh
AU  - Heidari F
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Farahmand, Ghasem
AU  - Farahmand G
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Bitaraf, Masoume
AU  - Bitaraf M
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Mirmiranpour, Hossein
AU  - Mirmiranpour H
AD  - Department of Clinical Biochemistry, School of Medicine, Tehran University of 
      Medical Sciences, Tehran, Iran.
FAU - Esteghamati, Alireza
AU  - Esteghamati A
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Nakhjavani, Manouchehr
AU  - Nakhjavani M
AD  - Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran 
      University of Medical Sciences, Tehran, Iran. nakhjavanim@tums.ac.ir.
LA  - eng
GR  - 963276/National Institute for Medical Research Development/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200603
PL  - United States
TA  - Endocrine
JT  - Endocrine
JID - 9434444
RN  - 0 (Biomarkers)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Biomarkers
MH  - C-Reactive Protein
MH  - *Diabetes Mellitus, Type 2/complications
MH  - *Diabetic Retinopathy
MH  - Glycated Hemoglobin
MH  - Humans
MH  - Nitric Oxide
MH  - Tumor Necrosis Factor-alpha
OTO - NOTNLM
OT  - Diabetic retinopathy
OT  - High-sensitivity C-reactive protein
OT  - Inflammation
OT  - Nitric oxide
OT  - Tumor necrosis factor alpha
OT  - Type 2 diabetes
EDAT- 2020/06/05 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/06/05 06:00
PHST- 2020/01/14 00:00 [received]
PHST- 2020/05/15 00:00 [accepted]
PHST- 2020/06/05 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/06/05 06:00 [entrez]
AID - 10.1007/s12020-020-02353-x [pii]
AID - 10.1007/s12020-020-02353-x [doi]
PST - ppublish
SO  - Endocrine. 2020 Sep;69(3):536-541. doi: 10.1007/s12020-020-02353-x. Epub 2020 Jun 
      3.