PMID- 32495882
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 10
DP  - 2020 May
TI  - CRKL promotes proliferation, migration, invasion of laryngeal squamous cell 
      carcinoma.
PG  - 5473-5480
LID - 21332 [pii]
LID - 10.26355/eurrev_202005_21332 [doi]
AB  - OBJECTIVE: This study aimed to investigate the expression and role of CT10 
      regulated kinase like (CRKL) in human laryngeal squamous cell carcinoma (LSCC) 
      progression. PATIENTS AND METHODS: Seventy-four laryngeal cancer cases were 
      detected by the immunohistochemistry S-P method and the results were analyzed. 
      RNA interference was used to downregulate the expression of CRKL in Hep-2 cells. 
      The silencing efficiency was detected by real-time PCR and Western blotting. The 
      cell proliferation, migration, and invasion after transfection were detected by 
      MTT, wound healing assay, transwell invasion assay, and apoptosis assay. Western 
      blot was conducted to determine the function of CRKL/epithelial-mesenchymal 
      transition (EMT) signaling pathway. RESULTS: The expression of CRKL was higher in 
      LSCC tissues. Patients with higher CRKL expression were correlated with lymph 
      node metastasis and postoperative survival rates. CRKL promoted proliferation, 
      migration, and invasion of Hep-2 cells in vitro. CONCLUSIONS: These findings 
      suggested that CRKL gene silencing significantly inhibited the proliferation, 
      migration, invasion, and EMT signaling pathway of Hep-2 cells. CRKL is considered 
      to be a new target for the treatment of LSCC.
FAU - Yu, M
AU  - Yu M
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing, China. yuming7272@163.com.
FAU - Gong, Z-P
AU  - Gong ZP
FAU - Ma, Z-L
AU  - Ma ZL
FAU - Yao, Z-F
AU  - Yao ZF
FAU - Liu, C
AU  - Liu C
FAU - Wang, J
AU  - Wang J
FAU - Li, Y-S
AU  - Li YS
FAU - Zhang, Z-X
AU  - Zhang ZX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CRKL protein)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism/*pathology
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/*pathology
MH  - Neoplasm Invasiveness
MH  - Tumor Cells, Cultured
EDAT- 2020/06/05 06:00
MHDA- 2021/03/31 06:00
CRDT- 2020/06/05 06:00
PHST- 2020/06/05 06:00 [entrez]
PHST- 2020/06/05 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
AID - 21332 [pii]
AID - 10.26355/eurrev_202005_21332 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 May;24(10):5473-5480. doi: 
      10.26355/eurrev_202005_21332.