PMID- 32498303
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 11
DP  - 2020 Jun 2
TI  - Characterization of miRNAs in Cultured Atlantic Salmon Head Kidney Monocyte-Like 
      and Macrophage-Like Cells.
LID - 10.3390/ijms21113989 [doi]
LID - 3989
AB  - Macrophages are among the first cells to respond to infection and disease. While 
      microRNAs (miRNAs) are involved in the process of monocyte-to-macrophage 
      differentiation in mammals, less is known in teleost fish. Here, Atlantic salmon 
      head kidney leukocytes (HKLs) were used to study the expression of miRNAs in 
      response to in vitro culture. The morphological analysis of cultures showed 
      predominantly monocyte-like cells on Day 1 and macrophage-like cells on Day 5, 
      suggesting that the HKLs had differentiated from monocytes to macrophages. Day 5 
      HKLs also contained a higher percentage of phagocytic cells. Small RNA sequencing 
      and qPCR analysis were applied to examine the miRNA diversity and expression. 
      There were 370 known mature Atlantic salmon miRNAs in HKLs. Twenty-two miRNAs (15 
      families) were downregulated while 44 miRNAs (25 families) were upregulated on 
      Day 5 vs. Day 1. Mammalian orthologs of many of the differentially expressed (DE) 
      miRNAs are known to regulate macrophage activation and differentiation, while the 
      teleost-specific miR-2188, miR-462 and miR-731 were also DE and are associated 
      with immune responses in fish. In silico predictions identified several putative 
      target genes of qPCR-validated miRNAs associated with vertebrate macrophage 
      differentiation. This study identified Atlantic salmon miRNAs likely to influence 
      macrophage differentiation, providing important knowledge for future functional 
      studies.
FAU - Smith, Nicole C
AU  - Smith NC
AD  - Department of Ocean Sciences, Memorial University of Newfoundland, 0 Marine Lab 
      Road, St. John's, NL A1C 5S7, Canada.
FAU - Christian, Sherri L
AU  - Christian SL
AUID- ORCID: 0000-0002-4289-4386
AD  - Department of Biochemistry, Memorial University of Newfoundland, 232 Elizabeth 
      Ave, St. John's, NL A1B 3X9, Canada.
FAU - Woldemariam, Nardos T
AU  - Woldemariam NT
AD  - Department of Life Sciences and Health, Faculty of Health Sciences, OsloMet-Oslo 
      Metropolitan University, N-0130 Oslo, Norway.
FAU - Clow, Kathy A
AU  - Clow KA
AD  - Department of Ocean Sciences, Memorial University of Newfoundland, 0 Marine Lab 
      Road, St. John's, NL A1C 5S7, Canada.
FAU - Rise, Matthew L
AU  - Rise ML
AD  - Department of Ocean Sciences, Memorial University of Newfoundland, 0 Marine Lab 
      Road, St. John's, NL A1C 5S7, Canada.
FAU - Andreassen, Rune
AU  - Andreassen R
AUID- ORCID: 0000-0002-8773-1273
AD  - Department of Life Sciences and Health, Faculty of Health Sciences, OsloMet-Oslo 
      Metropolitan University, N-0130 Oslo, Norway.
LA  - eng
GR  - 280839/E40/Norwegian Research Council grant to R.A./
GR  - HNP-2015/10026/High North Programme of Norway grant to R.A. and M.L.R/
GR  - Discovery Grant to M.L.R. (341304-2012)/Natural Sciences and Engineering Research 
      Council of Canada/
GR  - Discovery Grant to S.L.C. (2017-04630)/Natural Sciences and Engineering Research 
      Council of Canada/
GR  - PGS D fellowship to N.C.S./Natural Sciences and Engineering Research Council of 
      Canada/
GR  - GAPP project numbers 6604 and 6607 to M.L.R./Genome Canada/
PT  - Journal Article
DEP - 20200602
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Untranslated)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cluster Analysis
MH  - Gene Expression Profiling
MH  - Head Kidney/*cytology
MH  - Macrophages/*cytology
MH  - MicroRNAs/*genetics
MH  - Monocytes/*cytology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - RNA, Small Untranslated/genetics
MH  - Respiratory Burst
MH  - Salmo salar/*genetics
MH  - Software
MH  - Up-Regulation
PMC - PMC7312525
OTO - NOTNLM
OT  - Atlantic salmon
OT  - cell differentiation
OT  - head kidney culture
OT  - macrophages
OT  - miRNA
OT  - sequencing
COIS- The authors declare no conflict of interest.
EDAT- 2020/06/06 06:00
MHDA- 2021/03/31 06:00
PMCR- 2020/06/01
CRDT- 2020/06/06 06:00
PHST- 2020/04/29 00:00 [received]
PHST- 2020/05/26 00:00 [revised]
PHST- 2020/05/30 00:00 [accepted]
PHST- 2020/06/06 06:00 [entrez]
PHST- 2020/06/06 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - ijms21113989 [pii]
AID - ijms-21-03989 [pii]
AID - 10.3390/ijms21113989 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Jun 2;21(11):3989. doi: 10.3390/ijms21113989.