PMID- 32501408 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220414 IS - 1876-3820 (Print) IS - 1876-3839 (Electronic) IS - 1876-3820 (Linking) VI - 37 DP - 2020 Aug TI - Can network pharmacology identify the anti-virus and anti- inflammatory activities of Shuanghuanglian oral liquid used in Chinese medicine for respiratory tract infection? PG - 101139 LID - 10.1016/j.eujim.2020.101139 [doi] AB - INTRODUCTION: Shuanghuanglian (SHL) oral liquid is a well-known traditional Chinese medicine preparation administered for respiratory tract infections in China. However, the underlying pharmacological mechanisms remain unclear. The present study aims to determine the potential pharmacological mechanisms of SHL oral liquid based on network pharmacology. METHODS: Network pharmacology-based strategy including collection and analysis of putative compounds and target genes, network construction, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Gene Ontology (GO) enrichment, identification of key compounds and target genes, and molecule docking was performed in this study. RESULTS: A total of 82 bioactive compounds and 226 putative target genes of SHL oral liquid were collected. Of note, 28 hub target genes including 4 major hub target genes: estrogen receptor 1 (ESR1), nuclear receptor coactivator 2 (NCOA2), nuclear receptor coactivator 1 (NCOA1), androgen receptor (AR) and 5 key compounds (quercetin, luteolin, baicalein, kaempferol and wogonin) were identified based on network analysis. The hub target genes mainly enriched in pathways including PI3K-Akt signaling pathway, human cytomegalovirus infection, and human papillomavirus infection, which could be the underlying pharmacological mechanisms of SHL oral liquid for treating diseases. Moreover, the key compounds had great molecule docking binding affinity with the major hub target genes. CONCLUSION: Using network pharmacology analysis, SHL oral liquid was found to contain anti-virus, anti-inflammatory, and "multi-compounds and multi-targets" with therapeutic actions. These findings may provide a valuable direction for further clinical application and research. CI - (c) 2020 Elsevier GmbH. All rights reserved. FAU - Zhuang, Zhenjie AU - Zhuang Z AD - Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Wen, Junmao AU - Wen J AD - Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Zhang, Lu AU - Zhang L AD - Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Zhang, Mingjia AU - Zhang M AD - Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Zhong, Xiaoying AU - Zhong X AD - Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Chen, Huiqi AU - Chen H AD - Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Luo, Chuanjin AU - Luo C AD - The First Affiliated Hospital of Guangdong University of Chinese Medicine, No.12, Airport Road, Baiyun District, Guangzhou 510405, China. LA - eng PT - Journal Article DEP - 20200526 PL - Netherlands TA - Eur J Integr Med JT - European journal of integrative medicine JID - 101518381 PMC - PMC7255237 OTO - NOTNLM OT - AM, alveolar macrophages OT - AR, androgen receptor OT - CAS, Chemical abstracts service number OT - CFDA, The China Food and Drug Administration OT - COX, cyclooxygenases OT - COX-2, cyclooxygenase OT - DL, drug-likeness OT - ESR1, estrogen receptor 1 OT - Flos Lonicerae OT - Fructus Forsythiae OT - GO, Gene Ontology OT - HCMV, Human cytomegalovirus OT - HCV, human cytomegalovirus OT - HPV, Human papillomavirus OT - HQ, Huangqin, Radix Scutellariae OT - JYH, Jinyinhua, Flos Lonicerae OT - KEGG, Kyoto Encyclopedia of Genes and Genomes OT - LQ, Lianqiao, Fructus Forsythiae OT - MCP, monocyte chemoattractant protein OT - NCOA1, nuclear receptor coactivator 1 OT - NCOA2, nuclear receptor coactivator 2 OT - NO, nitric oxide OT - Network pharmacology OT - OB, oral bioavailability OT - PG, prostaglandin OT - Pharmacological mechanism OT - ROS, reactive oxygen species OT - RSV, respiratory syncytial virus OT - Radix Scutellariae OT - Respiratory tract infection OT - SARS-CoV, severe acute respiratory syndrome coronavirus OT - SHL oral liquid, Shuanghuanglian oral liquid OT - SMILES, Simplified molecular input line entry specification OT - Shuanghuanglian oral liquid OT - TCM, traditional Chinese medicine OT - TCMSP, Traditional Chinese Medicine Systems Pharmacology database COIS- The authors have no conflicts of interest to declare. EDAT- 2020/06/06 06:00 MHDA- 2020/06/06 06:01 PMCR- 2020/05/26 CRDT- 2020/06/06 06:00 PHST- 2020/04/08 00:00 [received] PHST- 2020/05/09 00:00 [revised] PHST- 2020/05/09 00:00 [accepted] PHST- 2020/06/06 06:00 [entrez] PHST- 2020/06/06 06:00 [pubmed] PHST- 2020/06/06 06:01 [medline] PHST- 2020/05/26 00:00 [pmc-release] AID - S1876-3820(20)30584-9 [pii] AID - 101139 [pii] AID - 10.1016/j.eujim.2020.101139 [doi] PST - ppublish SO - Eur J Integr Med. 2020 Aug;37:101139. doi: 10.1016/j.eujim.2020.101139. Epub 2020 May 26.